Effects of Dietary Supplementation with Fish Scales-Derived Collagen Peptides on Skin Parameters and Condition: A Randomized, Placebo-Controlled, Double-Blind Study

  • Seiko Koizumi
  • Naoki Inoue
  • Maiko Shimizu
  • Chang-ju Kwon
  • Hwa-young Kim
  • Kyoung Sik ParkEmail author


Fish scales-derived collagen peptides (CPs) are characterized by their specific amino acid composition with a high concentration of glycine, proline and hydroxyproline. These amino acids have been known to exert beneficial effects on human skin. The aim of the present study was to examine the effects of collagen peptides obtained from fish scales on changes in periorbital wrinkles, facial skin hydration, and skin elasticity in healthy women aged 30–60 years. In the present randomized, placebo-controlled, double-blind trial, 71 subjects consumed a 20 mL beverage containing 3000 mg of CPs or placebo once per day over 12 weeks. Significant decreases in periorbital wrinkles (p < 0.05) were observed in the treatment group after 12 weeks of CPs ingestion compared to the control group. This study also demonstrated a consistent trend of enhanced facial skin moisture (p < 0.001) and skin elasticity (p < 0.001) by dietary intake of CPs without any side effects or adverse events. These findings indicate that fish-derived CPs hold great promise as a natural supplement with cutaneous anti-aging properties.


Collagen hydrolysate Periorbital wrinkle Skin hydration Skin elasticity 



Alanine aminotransferase


Aspartate aminotransferase


Collagen peptides








Institutional review board


Mean corpuscular hemoglobin


Mean corpuscular hemoglobin concentration


Mean corpuscular volume






Red blood cell


Transepidermal water loss


White blood cell



We thank Dr. Tae Kee Moon from the clinical trial center of Ellead for conducting this clinical trial.


This study was funded by Ju Yeong NS Co. LTD. and Nitta Gelatin Inc. The sponsor had no access to the data, and data collection, study design, interpretation, and analyses were carried out independently by the clinical trial center of Ellead.

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee (IRB Number: EL-150113018A002) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Seiko Koizumi
    • 1
  • Naoki Inoue
    • 1
  • Maiko Shimizu
    • 1
  • Chang-ju Kwon
    • 2
  • Hwa-young Kim
    • 2
  • Kyoung Sik Park
    • 3
    Email author
  1. 1.Nitta Gelatin Inc.OsakaJapan
  2. 2.Ju Yeong NS Co. LTD.SeoulRepublic of Korea
  3. 3.Department of Biomedical Science, College of Natural Science and EngineeringCheongju University, CheongjuChungbukRepublic of Korea

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