Oxytocin Sustained Release Using Natural Rubber Latex Membranes
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The demand for biomaterials with properties that provide sustained release of substances with pharmacological interest is constant. One candidate for applications in this area is the Natural Rubber Latex (NRL) extracted from the rubber tree Hevea brasiliensis. Recent studies indicate the NRL as a matrix for sustained release, showing promising results for biomedical applications such as: can stimulate natural angiogenesis and is capable of adhering cells on its surface, promoting the replacement and regeneration of tissue. So, the NRL is an excellent candidate to propitiate the sustained release of peptides of pharmacological interest such as oxytocin, a hormonal peptide which has the function to promote uterine muscle contractions and reduce bleeding during childbirth, and stimulate the release of breast milk. Results demonstrated that 90 μg mL−1 (45 %) of the incorporated peptide in Natural Rubber Latex Biomedical (NRLb) functionalized membranes was released at 10 h in phosphate-buffered saline (PBS) solution. Swelling kinetics assay showed that the NRLb membranes are able to absorb over a period of 16 h up to 1.08 grams of water per grams of membrane. Scanning electron microscopy showed that the peptide was adsorbed on the surface and within NRLb membrane. Fourier transform infrared and Derivative Thermogravimetric analysis indicated that oxytocin did not interacted chemically with the membrane. Furthermore, hemolysis of erythrocytes, quantified spectrophotometrically using materials (Oxytocin, NRLb, and NRLb + Oxytocin) showed no hemolytic effects up to 100 μg mL−1 (compounds and mixtures), indicating no detectable disturbance of the red blood cell membranes. Based on these results it was possible to conclude that the NRLb has shown effectiveness as a model in the release of peptides with pharmacological interest.
KeywordsOxytocin Natural rubber latex Peptide Sustained release Biomaterial
This work was supported by CAPES, CNPq and FAPESP (Processes 2014/17526-8, 2015/02343-8).
Compliance with Ethical Standards
Conflicts of Interest
Author Natan Roberto de Barros declares that he has no conflict of interest. Author Matheus Carlos Romeiro Miranda declares that he has no conflict of interest. Author Felipe Azevedo Borges declares that he has no conflict of interest. Author Ricardo José de Mendonça declares that he has no conflict of interest. Author Eduardo Maffud Cilli declares that he has no conflict of interest. Author Rondinelli Donizetti Herculano declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Alves COM (2003) Teste da angiogênese estimulada por membranas de látex natural. University of São PauloGoogle Scholar
- Bozkurt O, Bayari SH, Severcan M, Krafft C, Popp J, Severcan F (2012) Structural alterations in rat liver proteins due to streptozotocin-induced diabetes and the recovery effect of selenium: Fourier transform infrared microspectroscopy and neural network study. J Biomed Opt. doi: 10.1117/1.JBO.17.7.076023 PubMedGoogle Scholar
- D’Auzac J, Jacob JL, Chrestin H (1989) Physiology of rubber tree: the composition of latex from Hevea brasiliensis as a laticiferous cytoplasm. CRC Press, Boca RatonGoogle Scholar
- Frade MAC, Cursi IB, Andrade FF, Coutinho-Netto J, Barbetta FM, Foss NT (2004) Management of diabetic skin wounds with a natural latex biomembrane. Med Cutan Ibero Lat Am 32(4):157–162Google Scholar
- Hasma H, Subramauian A (1986) Composition of lipids in latex of Hevea brasiliensis clone RRIM 501. J Nat Rubb Res. 1(1):30–40Google Scholar
- Miranda MCR (2014) Obtenção e estudos físico-químicos de biomembranas conjugadas de látex e peptídeos bioativos. Dissertation, UNESP - Univ Estadual PaulistaGoogle Scholar
- Salamon BF (2011) Controle de qualidade de cápsulas de cloridrato de venlafaxina manipuladas em farmácias magistrais de Porto Alegre – RS. Dissertation. UFRGS - Univ Fed Rio Grande do SulGoogle Scholar
- Steingräber T, Schtoltz T, Rodrigues PO (2008) Avaliação da influência de adjuvantes não-poliméricos solúveis na liberação do nimodipino a partir de formulações matriciais de liberação prolongada. Rev Colomb Ciencias Quim Farm 37(2):122–132Google Scholar
- World Health Organization (2015) WHO model list of essential medicines. http://www.who.int/medicines/publications/essentialmedicines/en. Accessed 12 January 2015