Antioxidant Defense of Betaine Against Oxidative Stress Induced by Ethanol in the Rat Testes
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Oxidative stress is one of the factors associated with decline in fertility and betaine has been shown to bear antioxidant and methyl donor properties in our recent studies. Thus, we designed the present study to examine antioxidant and methyl donor abilities of betaine in oxidative stress induced by ethanol in the rat testes. The adult male Sprague-Dawley rats were divided into four experimental groups and treated daily for 2 months as follows: control, ethanol (4 g/kg, orally), betaine (1.5 % of total diet, orally), and betaine plus ethanol (betaine, 1.5 % of total diet and after 120 min, ethanol 4 g/kg). Sperm motility and concentration significantly increased in betaine group when compared to the ethanol–treated rats. The main antioxidant enzyme (GPx) activity significantly increased (in order compensatory) in ethanol-treated rats when compared to betaine group while, antiperoxidative enzyme (CAT) activity significantly increased in betaine plus ethanol group as compared to ethanol-treated rats. Total homocysteine (tHcy) and TBARS concentration (as a lipid peroxidation marker) also significantly decreased in betaine and betaine plus ethanol groups as compared to ethanol-treated rats. Overall, methyl donor and antioxidant properties of betaine are promising and reduce the elevated tHcy and TBARS concentrations in betaine plus ethanol group. Therefore, betaine might be used as a potential therapy in hyperhomocysteinemia and oxidative stress induced by ethanol in alcoholism.
KeywordsBetaine Testes Antioxidant status Ethanol Rat
This research was financially supported by School of Veterinary Medicine-Shiraz University, Shiraz, Iran. We are most grateful to Saeedeh Ahmadi for the kind technical assistance; also like to thank M. Shoaei and R. Shirazi (the member and manager of Aryadalman Company, Tehran, Iran) for providing betaine (Betafine®).
- Alirezaei M, Saeb M, Javidnia K, Nazifi S, Khalighyan N, Saeb S (2010) Betaine reduction of hyperhomocysteinemia and enhancement of 5-hydroxyindoleacetic acid in ethanol-induced hyperhomocysteinemia in rabbits. Afr J Biochem Res 4(11):246–254Google Scholar
- Alirezaei M, Saeb M, Javidnia K, Nazifi S, Saeb S (2011b) Hyperhomocysteinemia reduction in ethanol-fed rabbits by oral betaine. Comp Clin Pathol. doi: 10.1007/s00580-010-1110-6
- Alirezaei M, Kheradmand A, Heydari R, Tanideh N, Neamati S, Rashidipour M (2012) Oleuropein protects against ethanol-induced oxidative stress and modulates sperm quality in the rat testis. Mediterr J Nutr Metab :1–7. doi: 10.1007/s12349-011-0079-2
- Barak AJ, Beckenhauer HC, Tuma DJ (1985) Ethanol feeding inhibits the activity of hepatic N5-methyltetrahydrofolate: homocysteine methyltransferase in the rat. IRCS Med Sci 13:760–761Google Scholar
- Claiborne A (1985) Catalase activity. In: Greenwald RA (ed) CRC handbook of methods for oxygen radical research, Vol 1. CRC Press, Boca Raton, Florida, USA, pp 283–284Google Scholar
- Cravo ML, Gloria LM, Selhub J, Nadeau MR, Camilo ME, Resende MP, Cardoso JN, Leitao CN, Mira FC (1996) Hyperhomocysteinemia in chronic alcoholism: correlation with folate, vitamin B-12, and vitamin B-6 status. Am Soc Nutr 63:220–224Google Scholar
- Doreswamy K, Shrilatha B, Rajeshkumar T (2004) Nickel-induced oxidative stress in testis of mice: evidence of DNA damage and genotoxic effects. J Androl 25(6):103–996Google Scholar
- Halsted CH, Villanueva JA, Devlin AM, Niemela O, Parkkila S, Garrow TA, Wallock LM, Shigenaga MK, Melnyk S, James SJ (2002) Folate deficiency disturbs hepatic methionine metabolism and promotes liver injury in the ethanol-fed micropig. Proc Natl Acad Sci USA 99(15):10072–10077PubMedCrossRefGoogle Scholar
- Srikanth V, Malini T, Arunakaran J, Govindarajulu P, Balasubramanian K (1999) Effects of ethanol treatment on epididymal secretory products and sperm maturation in albino rats. J Pharmacol Exp Therap 288(2):509–515Google Scholar