Solid Phase Synthesis of Dual Labeled Peptides: Development of Cell Permeable Calpain Specific Substrates
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A step-by-step evaluation of dual-labeled FRET substrates for the protease calpain is reported. The study led to cell permeable selections, with optimized specificity and effectiveness for the target enzyme, and improved stability to non-specific degrading enzymes.
KeywordsBlack Hole Quencher CAL Fluor dye calpain substrates cell-based assay(s) FRET protease assay solid phase peptide synthesis
The authors gratefully acknowledge Ronald M. Cook for his support, and Mark Reddington for helpful discussions and development of many of the CAL Fluor dye spacer variations.
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