T-Cell Antigen Receptor-alpha Chain Transmembrane Peptides: Correlation between Structure and Function

  • Marina Ali
  • Noeris K. Salam
  • Michael Amon
  • Veronika Bender
  • David E. Hibbs
  • Nicholas Manolios
Article

DOI: 10.1007/s10989-006-9025-4

Cite this article as:
Ali, M., Salam, N.K., Amon, M. et al. Int J Pept Res Ther (2006) 12: 261. doi:10.1007/s10989-006-9025-4

Core peptide (CP) is a unique peptide derived from the transmembrane sequence of T cell antigen receptor (TCR)-alpha chain that is capable of inhibiting the immune response both invitro and in animal models of T cell mediated inflammation. CP contains two basic amino acids (lysine and arginine) in its sequence. The presence of these charged residues interspersed between hydrophobic amino acids is important for function. Here in an attempt to understand CP’s biophysical properties leading to activity we have synthesized a number of CP analogues and correlated their model structure with their biological activity. It became apparent that it is not only the charge of the amino acids but also the nature of the polar amino acids themselves and the topography and spacing between them by hydrophobic amino acids, creating a hydrophobic face, that are critical for CP function.

Keywords

Molecular modelling peptides T-cell antigen receptor membranes 

Abbreviations

CD

circular dichroism

CP

core peptide

DMSO

dimethyl sulphoxide

DMPC

1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine

DMPG

1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol

HPLC

high performance liquid chromatography

RU

response units

SPR

surface plasmon resonance

TCR

T cell antigen receptor

TNCG

truncated newton conjugate gradient

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Marina Ali
    • 1
  • Noeris K. Salam
    • 2
  • Michael Amon
    • 1
  • Veronika Bender
    • 1
  • David E. Hibbs
    • 2
  • Nicholas Manolios
    • 1
  1. 1.Department of RheumatologyWestmead Hospital, WestmeadSydneyAustralia
  2. 2.Faculty of PharmacyUniversity of SydneySydneyAustralia

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