Electrophoresis of Derivatized Polyethylene Glycols: A Useful Method for Monitoring of Reactions on Soluble Polymeric Carrier

  • Jaroslav Šebestík
  • Petr Niederhafner
  • Martin Šafařík
  • Jan Hlaváček
Article

Abstract

A useful method for monitoring of peptide synthesis on polyethylene glycol (PEG) was developed and is described in this paper. The course of reactions such as loading, acylation, detachment and others where a charge of reactant and product differ, can be easily determined. The loading of the first amino acid is quantified with errors fitting well to those from other known analytical methods.

Key words:

PEG mPEG cobalt staining electrophoresis colorimetry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bayer, E., Mutter, M. 1972Nature237512513CrossRefPubMedGoogle Scholar
  2. Bayer, E., Mutter, M., Uhmann, R., Polster, J., Mauser, H. 1974J. Am. Chem. Soc.9673337336CrossRefPubMedGoogle Scholar
  3. Bayer, E. 1991Angew. Chem. Int. Ed. Engl.30113129CrossRefGoogle Scholar
  4. Benaglia, M., Annunziata, R., Cinquini, M., Cozzi, F., Ressel, S. 1998J. Org. Chem.6386288629CrossRefGoogle Scholar
  5. Brown, E. G., Hayes, T. J. 1955Analyst80755767CrossRefGoogle Scholar
  6. Chen, S., Janda, K. D. 1997J. Am. Chem. Soc.11987248725Google Scholar
  7. Durrum, E. L. 1950J. Am. Chem. Soc.7229432948CrossRefGoogle Scholar
  8. Fischer, P. M., Zheleva, D. I. 2002J. Pept. Sci.8529542PubMedGoogle Scholar
  9. Gnamm, H. 1950Die Losungs und Weichmachungsmittel, Wiessenschaftliche Verlagsgesellschaft6StuttgartGermanyGoogle Scholar
  10. Gravert, D. J., Janda, K. D. 1997Chem. Rev.97489510CrossRefPubMedGoogle Scholar
  11. Han, H., Wolfe, M. M., Brenner, S., Janda, K. D. 1995Proc. Natl. Acad. Sci. USA9264196423PubMedGoogle Scholar
  12. Milwidsky, B. M. 1969Analyst94377386CrossRefGoogle Scholar
  13. Mutter, M., Hagenmaier, H., Bayer, E. 1971Angew. Chem.83883884Google Scholar
  14. Mutter, M., Uhmann, R., and Bayer E.: 1975, Liebigs Ann. Chem. 901–915.Google Scholar
  15. Mutter, M., Bayer, E. 1980

    The liquid-phase method for peptide synthesis

    Gross, E.Meienhofer, J. eds. The peptides, Vol. 2. Special methods in peptide synthesis, part AAcademic PressNew York285332
    Google Scholar
  16. Pillai, V. N. R., Mutter, M. 1981Acc. Chem. Res.14122130CrossRefGoogle Scholar
  17. Ribiere, E., Enjalbal, C., Aubagnac, J.-L., Yadav-Bhatnagar, N., Martinez, J., Lamaty, F. 2004J. Comb. Chem.6464467CrossRefPubMedGoogle Scholar
  18. Shannon, S. K., Barany, G. 2004J. Comb. Chem.6165170CrossRefPubMedGoogle Scholar
  19. Šebestík J.: 2003, in jsebestik@seznam.cz.Google Scholar
  20. Hoeve, J. A. 1948Rec. Trans. Chim. Pays-Bas67649Google Scholar
  21. Wentworth, P. Jr. and Janda, K. D.: 1999, Chem. Commun. 1917–1924.Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Jaroslav Šebestík
    • 1
  • Petr Niederhafner
    • 1
  • Martin Šafařík
    • 1
  • Jan Hlaváček
    • 1
  1. 1.Institute of Organic Chemistry and BiochemistryCzech Academy of SciencesPrague 6Czech Republic

Personalised recommendations