Electrophoresis of Derivatized Polyethylene Glycols: A Useful Method for Monitoring of Reactions on Soluble Polymeric Carrier

  • Jaroslav Šebestík
  • Petr Niederhafner
  • Martin Šafařík
  • Jan Hlaváček


A useful method for monitoring of peptide synthesis on polyethylene glycol (PEG) was developed and is described in this paper. The course of reactions such as loading, acylation, detachment and others where a charge of reactant and product differ, can be easily determined. The loading of the first amino acid is quantified with errors fitting well to those from other known analytical methods.

Key words:

PEG mPEG cobalt staining electrophoresis colorimetry 


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Jaroslav Šebestík
    • 1
  • Petr Niederhafner
    • 1
  • Martin Šafařík
    • 1
  • Jan Hlaváček
    • 1
  1. 1.Institute of Organic Chemistry and BiochemistryCzech Academy of SciencesPrague 6Czech Republic

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