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Journal of Muscle Research and Cell Motility

, Volume 40, Issue 3–4, pp 373–378 | Cite as

Effects of adrenaline on contractility and endurance of isolated mammalian soleus with different calcium concentrations

  • Mudassir Haider RizviEmail author
  • Muhammad Abdul Azeem
  • Arifa Savanur
Original Article
  • 72 Downloads

Abstract

The β-adrenergic receptor stimulation improves endurance in fast twitch muscles and these effects are sensitive to extracellular Ca2+ influx. Present study is aimed to determine the effects of adrenaline, with different concentrations of extracellular Ca2+\(\left( {{\text{Ca}}_{\text{ECF}}^{ 2+ } } \right)\), on the contractility and endurance of slow twitch muscles during high frequency stimulations (HFS). Isolated soleus of rabbit was electrically stimulated (strength; 50 Hz, duration; 0.5 ms) in the presence (Test) of adrenaline (1 × 10−7 mM) or without adrenaline (CTL). Fatigue was induced with HFS (80 Hz) for the duration of 20 s. Contractions were recorded through isometric transducer connected with Powerlab. Kreb’s buffer was used with three compositions: standard with 2.5 mM Ca2+ (Ca-S), Ca2+ free buffer (Ca-F) and buffer with raised Ca2+ i.e., 10 mM (Ca-R). Muscles endurance was assessed by measuring the decline in tetanic tension in the terms of percentage (%Pmax) and rate of decline in tetanic tension (dP/dt). During 20 s, %Pmax showed reduction of only 10% in Ca-S. This decline was enhanced in Ca-F (50%) and reduced in Ca-R (6%). Effect of adrenaline was observed only in Ca-F where %Pmax was about 20% greater in Test than CTL. These effects were not observed in both Ca-S and Ca-R during 20 s. However, when duration of stimulation was increased to 120 or 150 s in Ca-S and Ca-R respectively, decline in %Pmax was less in Test as compared to CTL. Thus, \({\text{Ca}}_{\text{ECF}}^{ 2+ }\) plays protective role against fatigue during continuous HFS in slow twitch muscles. In addition, adrenaline improves the muscles endurance during fatiguing contraction but these effects are not mediated through \({\text{Ca}}_{\text{ECF}}^{ 2+ }\) influx.

Keywords

Adrenaline Slow twitch muscles Calcium transients High frequency stimulation 

Notes

Acknowledgements

This study was funded by Higher Education Commission Pakistan.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of PhysiologyUniversity of KarachiKarachiPakistan
  2. 2.Department of PhysiologyUnited Medical CollegeKarachiPakistan

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