Improving human skeletal muscle myosin heavy chain fiber typing efficiency

  • Kevin A. Murach
  • James R. Bagley
  • Kathryn A. McLeland
  • Jose A. Arevalo
  • Anthony B. Ciccone
  • Kylie K. Malyszek
  • Yuan Wen
  • Andrew J. Galpin
Rapid Communication

Abstract

Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscle biopsies were obtained from the vastus lateralis (VL) of nine resistance-trained males (25 ± 1 year, height = 179 ± 5 cm, mass = 82 ± 8 kg). Single fiber MHC composition was determined via SDS-PAGE. VL fiber type distribution [percent MHC I, I/IIa, IIa, IIa/IIx, and total “hybrids” (i.e. I/IIa + IIa/IIx)] was evaluated according to number of fibers analyzed per person (25 vs. 125). VL fiber type distribution did not differ according to number of fibers analyzed (P > 0.05). VL biopsy fiber type distribution of nine subjects is represented by analyzing 25 fibers per person. These data may help minimize cost, personnel-time, and materials associated with this technique, thereby improving fiber typing efficiency in humans.

Keywords

SDS-PAGE Muscle biopsy Hybrid fibers Vastus lateralis 

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Kevin A. Murach
    • 1
  • James R. Bagley
    • 2
  • Kathryn A. McLeland
    • 3
  • Jose A. Arevalo
    • 3
  • Anthony B. Ciccone
    • 3
  • Kylie K. Malyszek
    • 3
  • Yuan Wen
    • 4
  • Andrew J. Galpin
    • 3
  1. 1.Department of Rehabilitation Sciences, Center for Muscle Biology, College of Health SciencesUniversity of Kentucky, MS-508 Chandler Medical CenterLexingtonUSA
  2. 2.Muscle Physiology Laboratory, Department of KinesiologySan Francisco State UniversitySan FranciscoUSA
  3. 3.Biochemistry and Molecular Exercise Physiology Laboratory, Department of Kinesiology, Center for Sport PerformanceCalifornia State University, FullertonFullertonUSA
  4. 4.Department of PhysiologyUniversity of KentuckyLexingtonUSA

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