Improving human skeletal muscle myosin heavy chain fiber typing efficiency
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Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscle biopsies were obtained from the vastus lateralis (VL) of nine resistance-trained males (25 ± 1 year, height = 179 ± 5 cm, mass = 82 ± 8 kg). Single fiber MHC composition was determined via SDS-PAGE. VL fiber type distribution [percent MHC I, I/IIa, IIa, IIa/IIx, and total “hybrids” (i.e. I/IIa + IIa/IIx)] was evaluated according to number of fibers analyzed per person (25 vs. 125). VL fiber type distribution did not differ according to number of fibers analyzed (P > 0.05). VL biopsy fiber type distribution of nine subjects is represented by analyzing 25 fibers per person. These data may help minimize cost, personnel-time, and materials associated with this technique, thereby improving fiber typing efficiency in humans.
KeywordsSDS-PAGE Muscle biopsy Hybrid fibers Vastus lateralis
Thanks to Drs. Scott Trappe, Todd Trappe, Ulrika Raue, and Holmes Finch for their thoughts and feedback on the topic. This work was funded by a California State University, Fullerton Junior Faculty Grant (CSUF03569) to A. J. Galpin and publication costs were defrayed by the Department of Kinesiology, College of Health and Social Sciences, San Francisco State University.
Compliance with ethical standards
Conflict of interest
The authors have no conflicts to declare.
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