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Journal of Muscle Research and Cell Motility

, Volume 35, Issue 2, pp 179–189 | Cite as

Three calpain isoforms are autolyzed in rat fast-twitch muscle after eccentric contractions

  • Keita Kanzaki
  • Mai Kuratani
  • Satoshi Matsunaga
  • Noriyuki Yanaka
  • Masanobu WadaEmail author
Original Paper

Abstract

The present study investigated changes in autolysis of three calpain isoforms in skeletal muscles undergoing eccentric contractions (ECC), leading to prolonged force deficits. Rat extensor digitorum longus and tibialis anterior muscles were exposed to 200-repeated ECC in situ, excised immediately after or 3 or 6 days after cessation of ECC, and used for measures of force output and for biochemical analyses. Full restoration of tetanic force in ECC-treated muscles was not attained until 6 days of recovery. Maximal calpain activity determined by a fluorogenic substrate was unaltered immediately after ECC, but increased to 313 and 450 % after 3 and 6 days, respectively. Increases in the amount of autolyzed calpain-3 were apparent immediately and developed progressively with recovery time, whereas elevations of autolyzed μ- and m-calpain occurred after 3 and 6 days, respectively. The protein content was augmented only in m-calpain. It is suggested that the three calpain isoforms may be involved in the dismantling, repair, remodeling and/or regeneration processes in ECC-treated muscles.

Keywords

Autolysis Proteolysis Stretched muscle contraction Muscle damage Isoform 

Notes

Acknowledgments

Financial support for this study was received from Grants-Aid for Scientific Research of Japan (No. 24500788; Wada).

Conflict of interest

We do not have any conflict of interest.

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Keita Kanzaki
    • 1
  • Mai Kuratani
    • 2
  • Satoshi Matsunaga
    • 3
  • Noriyuki Yanaka
    • 4
  • Masanobu Wada
    • 2
    Email author
  1. 1.Faculty of Food CultureKurashiki Sakuyo UniversityOkayamaJapan
  2. 2.Graduate School of Integrated Arts and SciencesHiroshima UniversityHiroshimaJapan
  3. 3.Faculty of Education and CultureMiyazaki UniversityMiyazakiJapan
  4. 4.Graduate School of Biosphere ScienceHiroshima UniversityHiroshimaJapan

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