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Myosin Mg-ATPase of molluscan muscles is slightly activated by F-actin under catch state in vitro

  • Akira Yamada
  • Maki Yoshio
  • Kazuhiro Oiwa
Original Paper

Abstract

Molluscan muscle twitchin, a titin/connectin-related giant protein, regulates interactions between actin and myosin filaments at low Ca2+ concentrations. When it is dephosphorylated, actin filaments tightly bind to myosin filaments, resulting in the catch state known as the state of high passive tension with very low energy consumption. Yet when twitchin is phosphorylated actin filaments detach from the myosin filaments, resulting in relaxation of the catch. Here, steady-state Mg-ATPase activities of purified myosin were measured under various conditions: without twitchin, with dephosphorylated twitchin, or with phosphorylated twitchin; with or without phalloidin-stabilized F-actin; and at various Ca2+ concentrations. At low Ca2+ concentration, Mg-ATPase was activated by F-actin only in the presence of dephosphorylated twitchin (catch state). The activation was about two orders lower than that fully activated by Ca2+ and F-actin. In the absence of F-actin, twitchin and its phosphorylation state did not affect Mg-ATPase activities in any of the conditions we tested. Based on these results, we propose a molecular mechanism for the catch, where twitchin alone does not interact with the myosin catalytic motor domain but its complex with F-actin does, forming the bridge between actin and myosin filaments and the myosin slowly hydrolyzes Mg-ATP in the catch state.

Keywords

Catch muscle Catch state Myosin Mg-ATPase Twitchin Phosphorylation 

Notes

Acknowledgments

This work was supported by Japan Society for the Promotion of Science KAKENHI Grant No. 22570167 to A.Y.

Supplementary material

10974_2013_9339_MOESM1_ESM.pdf (192 kb)
Supplementary material 1 (PDF 192 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Advanced ICT Research Institute, National Institute of Information and Communications TechnologyKobeJapan

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