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Journal of Thermal Analysis and Calorimetry

, Volume 139, Issue 2, pp 1141–1149 | Cite as

Biothermokinetic characterization and evaluation on the quality of Colla corii asini

  • Jing Liu
  • Li Ma
  • Yang Li
  • Xiaobin Zuo
  • Yuan Gao
  • Tao Wang
  • Yan Zhang
  • Haibin Liu
  • Jiabo Wang
  • Ming NiuEmail author
  • Shijing LiuEmail author
  • Xiaohe XiaoEmail author
Article
  • 24 Downloads

Abstract

Colla corii asini (CCA) is a widely used animal-derived traditional medicine and nutritional supplement in China that has a variety of bioactive effects, especially anti-anaemic effects. To date, the effective substances in CCA are still unclear, thus limiting the evaluation of the quality and consistency of CCA. In this study, a microcalorimetry-based bioassay method was established to assess the quality of CCA by investigating its effects on the growth and metabolism of Escherichia coli, and ten biothermodynamic parameters were employed to characterize the biothermal changes. A principal component analysis (PCA) of these parameters showed that CCA significantly promotes the heat production of E. coli compared to that in the control group, and CCA showed an obvious dose–effect relationship from 0.625 to 20 mg mL−1. Then, the effects of 12 batches of CCA from three different manufacturers were studied at a concentration of 5 mg mL−1, which had a good effect and showed good intraday and daytime reproducibility. The results revealed distinctive characteristics among different manufacturers and good consistency within the same manufacturer according to the scores of the first principal component. In summary, the method based on microcalorimetric has good prospects in the evaluation of the quality of CCA.

Keywords

Colla corii asini Escherichia coli Microcalorimetry Biological assay Consistency 

Notes

Acknowledgements

This work was supported by the National Key R&D Program of China (No. 2018YFC1707000) and the National Standardization Construction Project of Traditional Chinese Medicine (Nos. ZYBZH-Y-SD-31, ZYBZH-Y-TJ-43, ZYBZH-Y-BJ-07, ZYBZH-C-HUN-21) and the National TCM Industry Science Technology Program (No. 201507002).

Author contributions

XHX, SJL, MN and JL participated in the research design; JL, LM and YL conducted the experiments; YZ and HBL contributed the samples; JL, MN, and XBZ provide analytical tools and revised the manuscript; JL, MN and JBW performed the data analysis; and YG and TW wrote or contributed to the writing of the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Commission C. Pharmacopoeia of the People’s Republic of China (part1). Beijing: China Medical Science Press; 2015. p. 189.Google Scholar
  2. 2.
    Tian JS, Zhang X, Liu H, Xiang H, Xing J, Zhang LZ, Qin XM. The hematinic effect of Colla corii asini (Ejiao) using 1H-NMR metabolomics coupled with correlation analysis in APH-induced anemic rats. RSC Adv. 2017;7:8952–62.CrossRefGoogle Scholar
  3. 3.
    Wu HZ, Yang F, Cui SY, Qin YF, Liu JW, Zhang YX. Hematopoietic effect of fractions from the enzyme-digested Colla corii asini on mice with 5-fluorouracil induced anemia. Am J Chin Med. 2007;35:853–66.CrossRefGoogle Scholar
  4. 4.
    Li YF, He H, Yang LL, Li XY, Li DC, Luo SP. Therapeutic effect of Colla corii asini on improving anemia and hemoglobin compositions in pregnant women with thalassemia. Int J Hematol. 2016;104:559–65.CrossRefGoogle Scholar
  5. 5.
    Yi N, Yang H, Wu Y, Xu FX. Research advances of pharmacology and effect of donkey hide gelatin. World Latest Med Inf. 2017;54:12–5.Google Scholar
  6. 6.
    Chen HH, Feng MJ, Zhu HF, Zhang L, Jiang RY, Zhang Y. Progress in pharmacological research of Asini Corii Colla. Chin J Drug Eval. 2014;31:23–6.Google Scholar
  7. 7.
    Xu XB, Guo SW, Hao XH, Ma H, Bai YP, Huang YQ. Improving antioxidant and antiproliferative activities of Colla corii asini hydrolysates using ginkgo biloba extracts. Food Sci Nutr. 2018;6:765–72.CrossRefGoogle Scholar
  8. 8.
    Li X, Shi F, Gong LP, Hang BJ, Li DY, Chi LL. Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach. Int J Nanomed. 2017;12:4443–54.CrossRefGoogle Scholar
  9. 9.
    Wang DL, Ru WW, Xu YP, Zhang JL, He XX, Fan GF, Mao BB, Zhou XS, Qin YF. Chemical constituents and bioactivities of Colla corii asini. Drug Discov Ther. 2014;8:201–7.CrossRefGoogle Scholar
  10. 10.
    Wu HZ, Ren CY, Yang F, Qin YF, Zhang YX, Liu JW. Extraction and identification of collagen-derived peptides with hematopoietic activity from Colla corii asini. J Ethnopharmacol. 2016;182:129–36.CrossRefGoogle Scholar
  11. 11.
    Li HB, Wu SH, Niu M, Wang JB, Xiao XH. History and development of biological assay for quality of chinese materia medica. Chin Tradit Herbal Drugs. 2017;48(14):2809–16.Google Scholar
  12. 12.
    U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. 2016. Botanical drug development guidance for industry. Pharmaceutical Quality/CMC.Google Scholar
  13. 13.
    Qin Y, Wang JB, Zhao YL, Shan LM, Li BC, Fang F, Jin C, Xiao XH. Establishment of a bioassay for the toxicity evaluation and quality control of Aconitum herbs. J Hazard Mater. 2012;199:350–7.CrossRefGoogle Scholar
  14. 14.
    Zhang DK, Li RS, Han X, Li CY, Zhao ZH, Zhang HZ, Yang M, Wang JB, Xiao XH. Toxic constituents index: a toxicity-calibrated quantitative evaluation approach for the precise toxicity prediction of the hypertoxic phytomedicine—aconite. Front Pharmacol. 2016;7:164.PubMedPubMedCentralGoogle Scholar
  15. 15.
    Yan D, Li JX, Xiong Y, Zhang CE, Luo JY, Han YM, Wang RL, Jin C, Qian H, Li JY, Qiu LL, Peng C, Lin YL, Song XA, Xiao XH. Promotion of quality standard of herbal medicine by constituent removing and adding. Sci Rep. 2014;4:3668.CrossRefGoogle Scholar
  16. 16.
    Han X, Zhang DK, Guo YM, Feng WW, Dong Q, Zhang CE, Zhou YF, Liu Y, Wang J, Zhao YL, Xiao XH, Yang M. Screening and evaluation of commonly-used anti-influenza Chinese herbal medicines based on anti-neuraminidase activity. Chin J Nat Med. 2016;14:794–800.PubMedGoogle Scholar
  17. 17.
    Zhang CE, Niu M, Li RY, Feng WW, Ma X, Dong Q, Ma ZJ, Li GQ, Meng YK, Wang Y, Yin P, He LZ, Li YM, Tan P, Zhao YL, Wang JB, Dong XP, Xiao XH. Untargeted metabolomics reveals dose-response characteristics for effect of rhubarb in a rat model of cholestasis. Front Pharmacol. 2016;7:85.PubMedPubMedCentralGoogle Scholar
  18. 18.
    Wang JB, Jin C, Li HF, Li HB, Xiao XH, Qu Y. Essential issues of bioassay methods for quality control of laxative traditional Chinese medicines. Acta Pharm Sin. 2009;5:500–5.Google Scholar
  19. 19.
    Yan D, Jin C, Xiao XH, Dong XP. Antimicrobial properties of berberines alkaloids in Coptis chinensis Franch by microcalorimetry. J Biochem Biophys Methods. 2008;70:845–9.CrossRefGoogle Scholar
  20. 20.
    Zhang HZ, Tan P, Liu ZJ, Wang J, Wang JB, Xiao XH. Activating blood biological potency assay and chemical fingerprintchromatogram applied to quality evaluation of rhubarb. Acta Pharm Sin. 2017;52:436–42.Google Scholar
  21. 21.
    Li HB, Yan D, Wang JB, Wang JY, Bei ZC, Wei L, Xiao XH. Biological evaluation of Radix Isatidis based on neuraminidase activity assay. Acta Pharm Sin. 2009;44:162–6.Google Scholar
  22. 22.
    Shi ZL, Liu ZJ, Liu CS, Wu MQ, Su HB, Ma X, Zang YM, Wang JB, Zhao YL, Xiao XH. Spectrum-effect relationships between chemical fingerprints and antibacterial effects of Lonicerae Japonicae Flos and Lonicerae Flos Base on UPLC and microcalorimetry. Front Pharmacol. 2016;7:12.CrossRefGoogle Scholar
  23. 23.
    Liu ZJ, Shi ZL, Tu C, Zhang HZ, Gao D, Li CY, He Q, Li RS, Guo YM, Niu M, Zhang CE, Ren YS, Zhen HS, Wang JB, Xiao XH. An activity-calibrated chemical standardization approach for quality evaluation of Salvia miltiorrhiza Bge. RSC Adv. 2016;7:5331–9.CrossRefGoogle Scholar
  24. 24.
    Kabanova N, Stulova I, Vilu R. Microcalorimetric study of the growth of bacterial colonies of Lactococcus lactis IL1403 in agar gels. Food Microbiol. 2012;29:67–79.CrossRefGoogle Scholar
  25. 25.
    Jiang DJ, Zhang L, Cao YD, Yao WF. Application of gut microbiota in research of Chinese medicines. China J Chin Materia Med. 2016;41:3218–25.Google Scholar
  26. 26.
    Zhou DL, Yan D, Li BC, Wu YS, Xiao XH. Investigation of metabolic action of Cordyceps sinensis and its cultured mycelia on Escherichia coli by microcalorimetry. Acta Pharm Sin. 2009;6:640–4.Google Scholar
  27. 27.
    Lee J, Lee E, Kim D, Lee J, Yoo H, Koh B. Studies on absorption, distribution and metabolism of ginseng in humans after oral administration. J Ethnopharmacol. 2009;122:143–8.CrossRefGoogle Scholar
  28. 28.
    Guo SH. The Colla Corii Asini suppository for the treatment of chronic ulcerative colitis. Chin J Integr Tradit Western Med. 1989;3:178–178.Google Scholar
  29. 29.
    Zhang HZ. Method for ass-skin-glue colon administration preparation and use thereof. CN1850114A [P]. 2006.Google Scholar
  30. 30.
    Zhang HZ. Enteric ass-hide gelatin prepn and its prepn process. CN1771994 [P]. 2006.Google Scholar
  31. 31.
    Yan D, Wei L, Xiao XH, Zhou DL, Han YM. Microcalorimetric investigation of effect of berberine alkaloids from Coptis chinensis Franch on intestinal diagnostic flora growth. Chin Sci Bull. 2009;54:369–73.Google Scholar
  32. 32.
    Ministry of Health, Labour & Welfare. Japanese pharmacopeia. 14th ed. Tokyo: Ministry of Health, Labour & Welfare; 2001. p. 54–9.Google Scholar
  33. 33.
    USP 30-NF25. The United States pharmacopoeia-The National Formulary. Rockville, MD. The United States Pharmacopoeial Convention, Inc., 2007. p 103.Google Scholar
  34. 34.
    Fuertes G, Sánchez-Muñoz OL, Pedrueza E, Abderrafi K, Salgado J, Jiménez E. Switchable effects from novel silica-coated silver nanoparticles bactericidal mediated by light irradiation. Langmuir. 2011;27(6):2826–33.CrossRefGoogle Scholar
  35. 35.
    Yan D, Han YM, Luo JY, Zhang P, Tang HY, Peng C, Xiao XH. Biological assay of animal cornu medicin quality based on microcalorimetry and chemometrics. Chin Sci Bull. 2010;55:3193–8.CrossRefGoogle Scholar
  36. 36.
    Chen DX, Zhang K, Li JK, Zeng C, Huang QX, Nie YZ, Zhu JY, Gan M. Microcalorimetric investigation of the ATPase activity and the refolding activity of GroEL system. J Therm Anal Calorim. 2019;135(2):2411–8.CrossRefGoogle Scholar
  37. 37.
    Wang T, Zhou XL, Zou WJ, Zhang P, Wang JP, Li HT, Wei SZ, Li L, Gao JY, Li YZ, Zhao YL. Synergistic effects of Ginseng C.A. Mey and Astragalus membranaceus (Fisch.) Bunge on activating mice splenic lymphocytes detected by microcalorimetry and the underlying mechanisms predicted by in silico network analysis. J Therm Anal Calorim. 2018;132(3):1933–42.CrossRefGoogle Scholar
  38. 38.
    Editor Group. Is Colla corii asini just boiled donkey skin? Tradit Med Res. 2018;3:3.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  1. 1.Chengde Medical CollegeChengdeChina
  2. 2.Department of China Military Institute of Chinese Materia, the Fifth Medical CentreChinese PLA (People’s Liberation Army) General HospitalBeijingChina
  3. 3.School of Traditional Chinese MedicineCapital Medical UniversityBeijingChina
  4. 4.National Engineering Research Center for Gelatin-Based Traditional Chinese MedicineShandongChina
  5. 5.Department of Integrative Medical Center, the Fifth Medical CentreChinese PLA (People’s Liberation Army) General HospitalBeijingChina

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