Journal of Thermal Analysis and Calorimetry

, Volume 139, Issue 2, pp 1141–1149 | Cite as

Biothermokinetic characterization and evaluation on the quality of Colla corii asini

  • Jing Liu
  • Li Ma
  • Yang Li
  • Xiaobin Zuo
  • Yuan Gao
  • Tao Wang
  • Yan Zhang
  • Haibin Liu
  • Jiabo Wang
  • Ming NiuEmail author
  • Shijing LiuEmail author
  • Xiaohe XiaoEmail author


Colla corii asini (CCA) is a widely used animal-derived traditional medicine and nutritional supplement in China that has a variety of bioactive effects, especially anti-anaemic effects. To date, the effective substances in CCA are still unclear, thus limiting the evaluation of the quality and consistency of CCA. In this study, a microcalorimetry-based bioassay method was established to assess the quality of CCA by investigating its effects on the growth and metabolism of Escherichia coli, and ten biothermodynamic parameters were employed to characterize the biothermal changes. A principal component analysis (PCA) of these parameters showed that CCA significantly promotes the heat production of E. coli compared to that in the control group, and CCA showed an obvious dose–effect relationship from 0.625 to 20 mg mL−1. Then, the effects of 12 batches of CCA from three different manufacturers were studied at a concentration of 5 mg mL−1, which had a good effect and showed good intraday and daytime reproducibility. The results revealed distinctive characteristics among different manufacturers and good consistency within the same manufacturer according to the scores of the first principal component. In summary, the method based on microcalorimetric has good prospects in the evaluation of the quality of CCA.


Colla corii asini Escherichia coli Microcalorimetry Biological assay Consistency 



This work was supported by the National Key R&D Program of China (No. 2018YFC1707000) and the National Standardization Construction Project of Traditional Chinese Medicine (Nos. ZYBZH-Y-SD-31, ZYBZH-Y-TJ-43, ZYBZH-Y-BJ-07, ZYBZH-C-HUN-21) and the National TCM Industry Science Technology Program (No. 201507002).

Author contributions

XHX, SJL, MN and JL participated in the research design; JL, LM and YL conducted the experiments; YZ and HBL contributed the samples; JL, MN, and XBZ provide analytical tools and revised the manuscript; JL, MN and JBW performed the data analysis; and YG and TW wrote or contributed to the writing of the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  1. 1.Chengde Medical CollegeChengdeChina
  2. 2.Department of China Military Institute of Chinese Materia, the Fifth Medical CentreChinese PLA (People’s Liberation Army) General HospitalBeijingChina
  3. 3.School of Traditional Chinese MedicineCapital Medical UniversityBeijingChina
  4. 4.National Engineering Research Center for Gelatin-Based Traditional Chinese MedicineShandongChina
  5. 5.Department of Integrative Medical Center, the Fifth Medical CentreChinese PLA (People’s Liberation Army) General HospitalBeijingChina

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