Biothermokinetic characterization and evaluation on the quality of Colla corii asini
- 24 Downloads
Colla corii asini (CCA) is a widely used animal-derived traditional medicine and nutritional supplement in China that has a variety of bioactive effects, especially anti-anaemic effects. To date, the effective substances in CCA are still unclear, thus limiting the evaluation of the quality and consistency of CCA. In this study, a microcalorimetry-based bioassay method was established to assess the quality of CCA by investigating its effects on the growth and metabolism of Escherichia coli, and ten biothermodynamic parameters were employed to characterize the biothermal changes. A principal component analysis (PCA) of these parameters showed that CCA significantly promotes the heat production of E. coli compared to that in the control group, and CCA showed an obvious dose–effect relationship from 0.625 to 20 mg mL−1. Then, the effects of 12 batches of CCA from three different manufacturers were studied at a concentration of 5 mg mL−1, which had a good effect and showed good intraday and daytime reproducibility. The results revealed distinctive characteristics among different manufacturers and good consistency within the same manufacturer according to the scores of the first principal component. In summary, the method based on microcalorimetric has good prospects in the evaluation of the quality of CCA.
KeywordsColla corii asini Escherichia coli Microcalorimetry Biological assay Consistency
This work was supported by the National Key R&D Program of China (No. 2018YFC1707000) and the National Standardization Construction Project of Traditional Chinese Medicine (Nos. ZYBZH-Y-SD-31, ZYBZH-Y-TJ-43, ZYBZH-Y-BJ-07, ZYBZH-C-HUN-21) and the National TCM Industry Science Technology Program (No. 201507002).
XHX, SJL, MN and JL participated in the research design; JL, LM and YL conducted the experiments; YZ and HBL contributed the samples; JL, MN, and XBZ provide analytical tools and revised the manuscript; JL, MN and JBW performed the data analysis; and YG and TW wrote or contributed to the writing of the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Commission C. Pharmacopoeia of the People’s Republic of China (part1). Beijing: China Medical Science Press; 2015. p. 189.Google Scholar
- 5.Yi N, Yang H, Wu Y, Xu FX. Research advances of pharmacology and effect of donkey hide gelatin. World Latest Med Inf. 2017;54:12–5.Google Scholar
- 6.Chen HH, Feng MJ, Zhu HF, Zhang L, Jiang RY, Zhang Y. Progress in pharmacological research of Asini Corii Colla. Chin J Drug Eval. 2014;31:23–6.Google Scholar
- 11.Li HB, Wu SH, Niu M, Wang JB, Xiao XH. History and development of biological assay for quality of chinese materia medica. Chin Tradit Herbal Drugs. 2017;48(14):2809–16.Google Scholar
- 12.U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. 2016. Botanical drug development guidance for industry. Pharmaceutical Quality/CMC.Google Scholar
- 17.Zhang CE, Niu M, Li RY, Feng WW, Ma X, Dong Q, Ma ZJ, Li GQ, Meng YK, Wang Y, Yin P, He LZ, Li YM, Tan P, Zhao YL, Wang JB, Dong XP, Xiao XH. Untargeted metabolomics reveals dose-response characteristics for effect of rhubarb in a rat model of cholestasis. Front Pharmacol. 2016;7:85.PubMedPubMedCentralGoogle Scholar
- 18.Wang JB, Jin C, Li HF, Li HB, Xiao XH, Qu Y. Essential issues of bioassay methods for quality control of laxative traditional Chinese medicines. Acta Pharm Sin. 2009;5:500–5.Google Scholar
- 20.Zhang HZ, Tan P, Liu ZJ, Wang J, Wang JB, Xiao XH. Activating blood biological potency assay and chemical fingerprintchromatogram applied to quality evaluation of rhubarb. Acta Pharm Sin. 2017;52:436–42.Google Scholar
- 21.Li HB, Yan D, Wang JB, Wang JY, Bei ZC, Wei L, Xiao XH. Biological evaluation of Radix Isatidis based on neuraminidase activity assay. Acta Pharm Sin. 2009;44:162–6.Google Scholar
- 25.Jiang DJ, Zhang L, Cao YD, Yao WF. Application of gut microbiota in research of Chinese medicines. China J Chin Materia Med. 2016;41:3218–25.Google Scholar
- 26.Zhou DL, Yan D, Li BC, Wu YS, Xiao XH. Investigation of metabolic action of Cordyceps sinensis and its cultured mycelia on Escherichia coli by microcalorimetry. Acta Pharm Sin. 2009;6:640–4.Google Scholar
- 28.Guo SH. The Colla Corii Asini suppository for the treatment of chronic ulcerative colitis. Chin J Integr Tradit Western Med. 1989;3:178–178.Google Scholar
- 29.Zhang HZ. Method for ass-skin-glue colon administration preparation and use thereof. CN1850114A [P]. 2006.Google Scholar
- 30.Zhang HZ. Enteric ass-hide gelatin prepn and its prepn process. CN1771994 [P]. 2006.Google Scholar
- 31.Yan D, Wei L, Xiao XH, Zhou DL, Han YM. Microcalorimetric investigation of effect of berberine alkaloids from Coptis chinensis Franch on intestinal diagnostic flora growth. Chin Sci Bull. 2009;54:369–73.Google Scholar
- 32.Ministry of Health, Labour & Welfare. Japanese pharmacopeia. 14th ed. Tokyo: Ministry of Health, Labour & Welfare; 2001. p. 54–9.Google Scholar
- 33.USP 30-NF25. The United States pharmacopoeia-The National Formulary. Rockville, MD. The United States Pharmacopoeial Convention, Inc., 2007. p 103.Google Scholar
- 37.Wang T, Zhou XL, Zou WJ, Zhang P, Wang JP, Li HT, Wei SZ, Li L, Gao JY, Li YZ, Zhao YL. Synergistic effects of Ginseng C.A. Mey and Astragalus membranaceus (Fisch.) Bunge on activating mice splenic lymphocytes detected by microcalorimetry and the underlying mechanisms predicted by in silico network analysis. J Therm Anal Calorim. 2018;132(3):1933–42.CrossRefGoogle Scholar
- 38.Editor Group. Is Colla corii asini just boiled donkey skin? Tradit Med Res. 2018;3:3.Google Scholar