Thermal profile of 4,4′-dinitrocarbanilide determined by thermogravimetry–differential scanning calorimetry–mass spectrometry (TG–DSC–MS) and pyrolysis–gas chromatography–mass spectrometry (Py–GC–MS)
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Nicarbazin is an anticoccidial used in poultry production. Its residue of concern left in chicken meat is 4,4′-dinitrocarbanilide (DNC) whose content can be reduced through meat thermal processing. Thermal analysis techniques can be considered to elucidate how DNC degradation proceeds. This study aimed to investigate the thermal behavior of DNC using TG–DSC–MS and Py–GC–MS. TG–DSC–MS data showed that DNC decomposition occurs in a single step (252–280 °C) as an endothermic event (ΔH of 670 J g−1). Fragments detected at m/z 43, 44 and 65 indicated the release of isocyanic acid, carbon dioxide and 4-nitroaniline as breakdown products. In addition, Py–GC–MS analysis confirmed that DNC is thermally degraded above 250 °C generating 4-nitrophenyl isocyanate and 4-nitroaniline as the major pyrolysis products. Findings obtained from thermal analysis herein are useful to better comprehend the DNC disappearance after submitting chicken meat to heat processing.
KeywordsThermal analysis Residue degradation Nicarbazin Anticoccidial Chicken meat Chemical residue
This work was supported by the Brazilian Agricultural Research Corporation [Embrapa, Grant No. 03.16.05.004.00.00], National Council for Scientific and Technological Development [CNPq, Grant No. 306930/2016-1] and the Coordination for the Improvement of Higher Education Personnel [CAPES, Grant No. 1533827 to D.M.B.].
- 1.EC. Commission Regulation (EU) No 875/2010 of 5 October 2010 concerning the authorisation for 10 years of an additive in feedingstuffs. Off J Eur Union. 2010;263:4–6.Google Scholar
- 2.USA. Title 21—Food and Drugs. Chapter I—Food and Drug Administration, Department of Health and Human services. Subchapter E—animal drugs, feeds, and related products part 556—tolerances for residues of new animal drugs in food. Gov. Publ. Off. 2012.Google Scholar
- 3.Brasil. Tabela de aditivos antimicrobianos, anticoccidianos e agonistas com uso autorizado na alimentação animal. [Table of antimicrobial additives, anticoccidials and agonists with authorized use in animal feed]. BRAZIL—Ministry of Agriculture, Livestock and Food Supply. 2015. p. 9.Google Scholar
- 5.EFSA. Opinion of the scientific panel on additives and products or substances used in animal feed on a request from the Commission on the efficacy and safety of the coccidiostat Koffogran. EFSA J. 2003;16:1–40.Google Scholar
- 7.BRASIL. Decreto No 9013 de 29 de março de 2017. Regulamenta a Lei no 1.283, de 18 de dezembro de 1950, e a Lei no 7.889, de 23 de novembro de 1989, que dispõem sobre a inspeção industrial e sanitária de produtos de origem animal. Brasília: MAPA; 2017. Available from: http://www.planalto.gov.br/ccivil_03/_Ato2015-2018/2017/Decreto/D9013.htm#art541. Accessed 17 Dec 2018.
- 8.FAO/WHO. Maximum residue limits (MRLs) and risk management recommendations (RMRs) for residues of veterinary drugs in foods. CAC/MRL 2-2017. Updated as at the 40th Session of the Codex Alimentarius Commission (July 2017). Rome; 2017.Google Scholar
- 16.Tian L. Thermal degradation of antibiotic residues: amphenicols as a case study. Montreal: McGill University; 2016.Google Scholar