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Journal of Thermal Analysis and Calorimetry

, Volume 130, Issue 3, pp 1707–1715 | Cite as

Microcalorimetric investigation of six alkaloids from Radix Aconite Lateralis Preparata (Fuzi) on the metabolic activity of mitochondria isolated from rat liver

  • Ding-kun Zhang
  • Zhi-rui Yang
  • Xue Han
  • Jun-zhi Lin
  • Chun-yu Li
  • Ming Yang
  • Jia-bo WangEmail author
  • Yan-ling ZhaoEmail author
  • Xiao-he XiaoEmail author
Article
  • 135 Downloads

Abstract

Using the TAM air isothermal microcalorimeter, the thermogenic power–time (HP–time) curves of isolated mitochondrial energy metabolism were measured, and the effects of six alkaloids from Radix Aconite Lateralis Preparata (Fuzi) including mesaconitine, aconitine, hypaconitine, benzoylmesaconine, benzoylaconine, and benzoylhypaconine on mitochondrial energy metabolism were investigated. Some quantitative information, such as k, P max, T max, Q, P av, and T lag, was obtained from the HP–time curves. The results revealed that mesaconitine, aconitine, and hypaconitine had inhibitory effect on rat-isolated mitochondrial energy metabolism, and their IC50 value was 1.82, 2.22, and 2.48 μg mL−1, respectively, while benzoylmesaconine, benzoylaconine, and benzoylhypaconine promoted mitochondrial energy metabolism, and their EC50 value was 30.95, 14.36, and more than 30 μg mL−1, respectively. Further structure–activity relationship analysis showed that the ester bond in C(8) position, hydroxyl in C(3) position, and ethyl in N atom could significantly influence the energy metabolism. These results provide scientific basis for the treatment of mitochondrial abnormalities and energy dysfunction diseases using Fuzi, and it will be of great interest to doctors and pharmacists.

Keywords

Radix Aconite Lateralis Preparata (Fuzi) Mitochondria Microcalorimetry Energy metabolism Diester diterpenoid alkaloids Monoester diterpenoid alkaloids 

Notes

Acknowledgements

We are grateful to the support of National Nature Science Fund (Nos. 81274026, 81403115, 81573631). We thank the reviewers for their critical comments on the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declared no conflict of interest.

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2017

Authors and Affiliations

  1. 1.College of PharmacyChengdu University of Traditional Chinese MedicineChengduPeople’s Republic of China
  2. 2.China Military Institute of Chinese MedicineBeijingPeople’s Republic of China
  3. 3.Key Laboratory of Modern Preparation of TCMJiangXi University of Traditional Chinese MedicineNanchangPeople’s Republic of China
  4. 4.Pharmacy DepartmentBeijingPeople’s Republic of China
  5. 5.Integrative Medical CenterBeijingPeople’s Republic of China
  6. 6.Sichuan Good Doctor Panxi Pharmaceutical Co., LTDXichangPeople’s Republic of China

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