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Journal of Thermal Analysis and Calorimetry

, Volume 108, Issue 1, pp 141–148 | Cite as

Thermal studies on protein isolates of white lupin seeds (Lupinus albus)

  • Gustavo Guadagnucci FontanariEmail author
  • José Manuel Martins
  • Marcelo Kobelnik
  • Iêda Aparecida Pastre
  • José Alfredo Gomes Arêas
  • José Paschoal Batistuti
  • Fernando Luis Fertonani
Article

Abstract

This study used TG, DSC, and SDS-PAGE techniques to study protein isolates (PIs) in the powder form obtained from lupin seeds flour Lupinus albus. Different methods of preparing PIs were tested, resulting in final products that were different only in relation to the yield and protein content. The results of the protein analysis by SDS-PAGE showed that the same protein fractions were present in the lupin seeds and in the obtained PIs. This result shows that the process of extraction was not damaging to the composition of the original protein. On the other hand, the results of the thermal analysis (DSC and TG–DTG curves) obtained for the different PIs, led to the detection of changes in the protein conformation through the ΔH values, which in general decreased with increasing values of pH and ionic strength in the experimental conditions of extraction.

Keywords

Lupinus albus TG DSC Protein isolate SDS-PAGE 

Notes

Acknowledgements

The authors wish to thank Predilecta Foods (São Lourenço do Turvo, SP, Brazil), CAPES Foundation (Brazil) for financial support, Ms Ana Paula Ferreira for help with lupin seed protein isolate extraction and Ms Maraiza Aparecida Silva for her technical assistance. The authors and this Foundation have no conflict of interest in regards to this manuscript.

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2011

Authors and Affiliations

  • Gustavo Guadagnucci Fontanari
    • 1
    • 2
    Email author
  • José Manuel Martins
    • 3
  • Marcelo Kobelnik
    • 4
  • Iêda Aparecida Pastre
    • 5
  • José Alfredo Gomes Arêas
    • 6
  • José Paschoal Batistuti
    • 1
  • Fernando Luis Fertonani
    • 5
  1. 1.Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição, UNESPAraraquaraBrazil
  2. 2.Faculdade de Saúde PúblicaDepartamento de Nutrição, USPSão PauloBrazil
  3. 3.Departamento de Zootecnia/ICAAMUniversidade de ÉvoraÉvoraPortugal
  4. 4.Centro Universitário do Norte Paulista—UNORPSão José do Rio PretoBrazil
  5. 5.IBILCE, UNESPSão José do Rio PretoBrazil
  6. 6.Faculdade de Saúde Pública, Departamento de Nutrição, USPSão PauloBrazil

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