Journal of Thermal Analysis and Calorimetry

, Volume 95, Issue 3, pp 709–712 | Cite as

Effect of phalloidin on the skeletal muscle ADP-actin filaments

  • Réka Dudás
  • Tünde Kupi
  • Andrea Vig
  • J. Orbán
  • D. Lőrinczy
Biochemical and Pharmaceutical Aspects

Abstract

The effect of phalloidin on the thermal stability of skeletal actin filaments polymerized from ADP-binding monomers was investigated with the method of differential scanning calorimetry. Phalloidin shifted the melting temperature of the ADP-F-actin from 59.1±1.0 to 80.0±1.2°C. The stabilizing effect of phalloidin propagated cooperatively along the filament. The cooperativity factor according to the applied model was 1.07±0.11. With these measurements it was possible to demonstrate that the binding of phalloidin has lower influence on the adjacent protomers in ADP- (k=1) than in ATP-actin filaments (k=3).

Keywords

ADP-actin filament calorimetry phalloidin stability thermodynamics 

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2009

Authors and Affiliations

  • Réka Dudás
    • 1
  • Tünde Kupi
    • 1
  • Andrea Vig
    • 1
  • J. Orbán
    • 1
  • D. Lőrinczy
    • 1
  1. 1.Department of BiophysicsUniversity of Pécs, Medical SchoolPécsHungary

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