Journal of Thermal Analysis and Calorimetry

, Volume 96, Issue 1, pp 301–305 | Cite as

Photochemical properties of simvastatin and lovastatin induced by radiation

  • Jadwiga Mielcarek
  • M. Naskrent
  • P. Grobelny
Regular Papers Green/Bio


HPLC and UV spectrophotometry were developed and validated for quantitative determination of two antihiperlipoproteinemia drugs, lovastatin and simvastatin.

Analytical performance parameters such as linearity, precision, specificity, limit of detection (LOD) and limit of quantification (LOQ) were determined according to the ICH Q1B guidelines. Chromatography was carried out by isocratic technique on a reversed-phase C-18 column. The UV spectroscopy determinations were performed at 238 nm. The linearity of the calibration curves in the desired concentration range was good (r(2)>0.999) for both HPLC and UV methods. The relative standard deviation (RSD) for these methods was <5%. Moreover, the precision obtained with HPLC correlated well with the UV results. The methods proposed are highly sensitive and precise. Other methods used for assessment of the photostability of the substances studied were electron paramagnetic resonance (EPR) and differential scanning calorimetry (DSC).


DSC EPR HMG-CoA HPLC lovastatin photodegradation simvastatin statins 


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  1. 1.
    R. Paoletti, A. Corsini and S. Bellosta, Atherosclerosis Supplements, 3 (2002) 35.CrossRefGoogle Scholar
  2. 2.
    A. S. Wierzbicki, R. Poston and A. Ferro, Pharmacology and Therapeutics, 99 (2003) 95.CrossRefGoogle Scholar
  3. 3.
    G. Schmitz and T. Langmann, Vascular Pharmacology, 44 (2006) 75.CrossRefGoogle Scholar
  4. 4.
    G. Beck, K. Kesseler, E. Baader, W. Bartmann, A. Bergmann, E. Granzer, H. Jendralla, B. Von Kerekjarto, R. Krause, G. Beck, K. Kesseler, E. Baader, W. Bartmann, A. Bergmann, E. Granzer, H. Jendralla, B. Von Kerekjarto and R. Krause, J. Med. Chem., 33 (1990) 52.CrossRefGoogle Scholar
  5. 5.
    M. Takemoto and J. K. Liao, Arterioscler. Thromb. Vasc. Biol., 21 (2001) 1712.CrossRefGoogle Scholar
  6. 6.
    E. Istvan, Atheroscler. Suppl., 4 (2003) 3.CrossRefGoogle Scholar
  7. 7.
    A. Endo, Atheroscler. Suppl., 5 (2004) 67.CrossRefGoogle Scholar
  8. 8.
    T. Wajima, S. Makita and K. Oshima, Clinical and Experimental Pharmacology and Physiology, 30 (2003) 958.CrossRefGoogle Scholar
  9. 9.
    W. Aman, W. Aman and K. Thoma, Pharmazie, 58 (2003) 877.Google Scholar
  10. 10.
    B. Glass, Cs. Novák and M. Brown, J. Therm. Anal. Cal., 77 (2004) 1013.CrossRefGoogle Scholar
  11. 11.
    T. R. Hurley, C. E. Colson, S. A. Clipper, S. E. Uhlendorf and M. D. Reily, Tetrahedron, 49 (1993) 1979.CrossRefGoogle Scholar
  12. 12.
    M. W. Lam and S. A. Mabury, Aquatic Sciences — Research Across Boundaries, 67 (2005) 177.Google Scholar
  13. 13.
    J. Mielcarek, M. Kula, R. Zych and P. Grobelny, Reaction Kinetics and Catalysis Letters, 86 (2005) 119.CrossRefGoogle Scholar
  14. 14.
    G. Miolo, S. Caffieri, L. Levorato, M. Imbesi, P. Giusti, T. Uz, R. Manev and H. Manev, Eur. J. Pharmacol., 450 (2002) 223.CrossRefGoogle Scholar
  15. 15.
    B. Marciniec, M. Stawny, M. Kozak and M. Naskrent, J. Therm. Anal. Cal., 84 (2006) 741.CrossRefGoogle Scholar
  16. 16.
    B. Marciniec, M. Kozak, M. Naskrent, K. Dettlaff, M. Ogrodowczyk, M. Stawny and L. Wachowski, J. Therm. Anal. Cal., 88 (2007) 337.CrossRefGoogle Scholar
  17. 17.
    M. Naskrent and J. Mielcarek, Spectrochim. Acta, Part A: Molecular and Biomolecular Spectroscopy, 67 (2007) 251.CrossRefGoogle Scholar
  18. 18.
    B. Marciniec, M. Stawny, M. Kozak and M. Naskrent, Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy, 69 (2008) 865.CrossRefGoogle Scholar
  19. 19.
    K. Dettlaff, B. Teżyk, B. Marciniec, R. Wachowiak, M. Naskrent and B. Bednarek, Chemical Analysis (Warsaw), 53 (2008) 171.Google Scholar
  20. 20.
    B. Marciniec, M. Stawny, M. Hoffmann and M. Naskrent, J. Therm. Anal. Cal., 93 (2008) 733.CrossRefGoogle Scholar
  21. 21.
    H. B. Ambroż, E. M. Kornacka, B. Marciniec, M. Ogrodowczyk and G. K. Przybytniak, Rad. Phys. Chem., 58 (2000) 357.CrossRefGoogle Scholar
  22. 22.
    R. Köseoğlu, E. Köseoğlu and F. Köksal, Appl. Rad. Isotopes, 58 (2003) 63.CrossRefGoogle Scholar
  23. 23.
    M. Gibella, A. S. Crucq, B. Tilquin, P. Stocker, G. Lesgards and J. Raffi, Rad. Phys. Chem., 58 (2000) 69.CrossRefGoogle Scholar
  24. 24.
    A. S. Crucq, Chim. Nouv., 12 (1994) 1356.Google Scholar
  25. 25.
    L. Varshney and K. M. Patel, Rad. Phys. Chem., 43 (1994) 471.CrossRefGoogle Scholar
  26. 26.
    N. G. S. Gopal, K. M. Patel, G. Sharma, H. L. Bhalla, P. A. Wills and N. Hilmy, Rad. Phys. Chem., 32 (1988) 619.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2009

Authors and Affiliations

  1. 1.Department of Inorganic and Analytical ChemistryPoznań University of Medical SciencesPoznańPoland
  2. 2.Department of Medical Physics, Faculty of PhysicsAdam Mickiewicz UniversityPoznańPoland

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