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Journal of Thermal Analysis and Calorimetry

, Volume 79, Issue 3, pp 645–648 | Cite as

Study on the nucleotide mutation in -35 box sequence of gene promoter by means of microcalorimetric method

Article

Summary

The important thermochemistry research method-microcalorimetry was successfully applied to study the effect of different nucleotide mutations in -35 box sequence on the gene promoter activity in <Emphasis Type=”Italic”>Escherichia coli</Emphasis>. The metabolic thermogenic curves of different <Emphasis Type=”Italic”>Escherichia coli</Emphasis> strains were obtained by microcalorimetric measurement using the TAM air Isothermal Microcalorimeter. By analyzing the data such as metabolic rate constant (<Emphasis Type=”Italic”>k</Emphasis><Subscript>m</Subscript>), inhibitory ratio (<Emphasis Type=”Italic”>I</Emphasis>) and half-inhibitory concentration of antibiotic (<Emphasis Type=”Italic”>IC</Emphasis><Subscript>50</Subscript>) obtained by the microcalorimetric method, it was found that the structure of -35 box sequence was very important for the gene promoter activity in <Emphasis Type=”Italic”>Escherichia coli</Emphasis>. Different nucleotide mutations in -35 box sequence had different effect on the gene promoter activity.

microcalorimetric method nucleotide mutation promoter activity 

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Copyright information

© Springer-Verlag/Akad&#233;miai Kiad&#243; 2005

Authors and Affiliations

  • P. Shen
    • 1
  1. 1.College of Life Sciences, Wuhan University

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