Journal of Thermal Analysis and Calorimetry

, Volume 79, Issue 1, pp 45–50 | Cite as

Microcalorimetric study of virus infection; The effects of hyperthermia and 1b recombinant homo interferon on the infection process of BHK-21 cells by foot and mouth disease virus

  • Z. Heng
  • Z. Congyi
  • W. Cunxin
  • W. Jibin
  • G. Chaojiang
  • L. Jie
  • L. Yuwen


The metabolic process of BHK-21 cell line infected by foot and mouth disease virus (FMDV) was determined by using LKB-2277 Bioactivity Monitor. The aim of the present study is to investigate the metabolic thermal power of the virus infection process of BHK-21 cells, the effects of combinational treatments of hyperthermia and 1b recombinant homo interferon on this process. In contrast to the metabolic thermal power of uninfected BHK-21 cells, the thermogenetic curves show that the energy metabolism mechanisms of BHK-21 cells were significantly changed by the virus infection process. The maximum thermal power decreased and the time needed to reach the maximal thermal power increased with the increasing concentration of interferon. The results also show that the infection process was thermosensitive. But no apparent synergetic effect of the combinational treatments of hyperthermia and interferon was observed. The present microcalorimetric results are in accordance with the cytomorphology observations.

metabolism microcalorimetry BHK-21 cell line FMD virus hyperthermia interferon 


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  1. 1.
    D. Esteban, B. Eric, E. Cristina and S. Francisco, Comp. Immunol. Microb., 25 (2002) 297.Google Scholar
  2. 2.
    E. H. Battley, J. Therm. Anal. Cal., 74 (2003) 709.CrossRefGoogle Scholar
  3. 3.
    R. B. Kemp, Handbook of Thermal Analysis and Calorimetry, Vol. 4, From Macromolecules to Man, Elsevier, Amsterdam 1999.Google Scholar
  4. 4.
    X.Y. Xie, C.X. Wang and Z.Y. Wang, J. Therm. Anal. Cal., 76 (2004) 275.CrossRefGoogle Scholar
  5. 5.
    H. Aki, J. Therm. Anal. Cal., 68 (2002) 553.CrossRefGoogle Scholar
  6. 6.
    B. Chardin, P. Gallice, J. C. Sari and M. Bruschi, J. Therm. Anal. Cal., 70 (2002) 475.CrossRefGoogle Scholar
  7. 7.
    R. B. Kemp, Thermochim. Acta, 193 (1991) 253.CrossRefGoogle Scholar
  8. 8.
    J. Nittinger and P. Fürst, Thermochim. Acta, 251 (1995) 155.CrossRefGoogle Scholar
  9. 9.
    R. B. Kemp and Y. Guan, Thermochim. Acta, 332 (1999) 203.CrossRefGoogle Scholar
  10. 10.
    Y. Xie, H. K. Joseph, W. Depierre and L. Nassberger, J. Pharmacol. Toxicol. Methods, 40 (1998) 137.CrossRefGoogle Scholar
  11. 11.
    A. M. Tan, B. Xu, S. Q. Huang and S. S. Qu, Thermochim. Acta, 333 (1999) 99.CrossRefGoogle Scholar
  12. 12.
    K. Ijungholm, I. Wadsö and L. Kjellen, Acta Path. Microbial. Scand. Sect. B, 86 (1978) 121.Google Scholar
  13. 13.
    A. M. Tan and J. H. Lu, J. Biochem. Biophys. Methods, 38 (1999) 225.CrossRefGoogle Scholar
  14. 14.
    J. Suurkuusk and I. Wadsö, Chem. Scr., 20 (1982) 155.Google Scholar
  15. 15.
    P. Backman, T. Kimura, A. Schon and I. Wadsö, J. Cell Physiol., 150 (1992) 99.Google Scholar
  16. 16.
    E. K. Wagner and M. J. Hewlett, Basic Virology, Blackwell Science Inc., Berlin 1999.Google Scholar
  17. 17.
    A. Isaacs and J. Lindenmann, Proc. R. Soc. Lond. B., 147 (1957) 258.Google Scholar
  18. 18.
    I. R. Tizard, The biology of veterinary cytokines interferons. In: M. J. Myers and M. P. Murtablgh (Eds), Cytokines in Animal Health and Disease. Marcel Dekker Inc, New York 1995.Google Scholar
  19. 19.
    G. J. Michael and G. K. Michael, Pharmacol. Therapeut., 78 (1998) 29.CrossRefGoogle Scholar
  20. 20.
    J. P. Mather and P. E. Roberts, Introduction to Cell and Tissue Culture: Theory and Technique, Plenum Press, New York 1998.Google Scholar
  21. 21.
    P. Lönnbro and A. Schön, Thermochim. Acta, 172 (1990) 75.CrossRefGoogle Scholar
  22. 22.
    Y. Feng, Z. F. Luo, S. S. Qu, C. Y. Zheng and H. Xu, Thermochim. Acta, 303 (1997) 203.CrossRefGoogle Scholar
  23. 23.
    L. S. Geoffrey, A. S. Julian and A. Antonio, Sem. Virol., 8 (1998) 409.CrossRefGoogle Scholar
  24. 24.
    H. Robins, W. L. I. Longo, R. A. Steeves, Lagoni, K. Rhonda, A. J. H. Anders, M. Ch. B. Neville, S. O’Keefe, W. Giese and C. L. Schmitt, Int. J. Radiation Oncology Biol. Phys., 15 (1988) 427.CrossRefGoogle Scholar
  25. 25.
    J. van der Zee, D. G. Gonzalez, G. C. van Rhoon, J. D. P. van Dijk, W. L. J. van Putten and A. A. M. Hart, The Lancet, 355 (2000) 1119.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2005

Authors and Affiliations

  • Z. Heng
    • 1
  • Z. Congyi
    • 2
  • W. Cunxin
    • 1
  • W. Jibin
    • 2
  • G. Chaojiang
    • 2
  • L. Jie
    • 1
  • L. Yuwen
    • 1
    • 2
  1. 1.College of Chemistry and Molecular ScienceWuhan University WuhanHubeiP. R. China
  2. 2.College of Life ScienceWuhan University WuhanHubeiP. R. China

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