Eggshell derived Se-doped HA nanorods for enhanced antitumor effect and curcumin delivery
- 70 Downloads
Using natural resources to prepare biomedical materials becomes the focus of current researches. This work presented the Se-doped HA nanorods, which were synthesized using waste eggshell by co-precipitation and hydrothermal route, aiming at the application of the therapy of bone tumor postoperatively. The synthetic products exhibited dispersive nanorods morphology with a length of about 50 nm and a width of 6 nm. And the physicochemical characterization indicated these nanorods with main phase of hydroxyapatite, and they exhibited excellent loading effect for curcumin and slowly stable controlled release fashion in the physiological buffers. 1.38% of the curcumin initially loaded into the nanorods was released into PBS solution over 159 h. Additionally, they exhibited good blood compatibility because of no hemolytic response and cytotoxicity with the healthy whole blood. Together, the Se-doped HA nanorods, not only possess the synthetic advantages such as simple-operation, resource-saving, pollution-free, but also serve as a promising nano-carrier for antitumor drug delivery applied in the onco-therapy of bone tumor and bone regenerative medicine.
A greener synthesis route was developed to fabricate the Se-doped HA nanorods.
Se doped HA nanorods showed steadily slow release fashion for curcumin.
Se doping into HA nanorods presented better blood compatibility and stronger antitumor effect on osteosarcoma.
KeywordsGreener synthesis Eggshell Hydroxyapatite Selenium Curcumin Delivery
We sincerely thank for the help from Dr. Huiyao Xiang from the first college of clinical medical science of China Three Gorges University for the constructive suggestion and data analysis about this study. This work was supported by National Natural Science Foundation of China (grant no. 81602559) and by Youth Science Fund Program of China Three Gorges University (grant no. 1115064).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 2.Behjati S, Tarpey PS, Haase K, Ye H, Young MD, Alexandrov LB, Farndon SJ, Collord G, Wedge DC, Martincorena I, Cooke SL, Davies H, Mifsud W, Lidgren M, Martin S, Latimer C, Maddison M, Butler AP, Teague JW, Pillay N, Shlien A, McDermott U, Futreal PA, Baumhoer D, Zaikova O, Bjerkehagen B, Myklebost O, Amary MF, Tirabosco R, Van Loo P, Stratton MR, Flanagan AM, Campbell PJ (2017) Recurrent mutation of IGF signalling genes and distinct patterns of genomic rearrangement in osteosarcoma. Nat Commun 8:15936–15943CrossRefGoogle Scholar
- 8.Thomas SC, Sharma H, Rawat P, Verma AK, Leekha A, Kumar V, Tyagi A, Gurjar BS, Iqbal Z, Talegaonkar S (2016) Synergistic anticancer efficacy of bendamustine hydrochloride loaded bioactive Hydroxyapatite nanoparticles: in-vitro, ex-vivo, and in-vivo evaluation. Colloids Surf B Biointerfaces 146:852–860CrossRefGoogle Scholar
- 15.Zhang ZH, Wen L, Wu QY, Chen C, Zheng R, Liu Q, Ni JZ, Song GL (2017) Long-term dietary supplementation with selenium-enriched yeast improves cognitive impairment, reverses synaptic deficits and mitigates tau pathology in a triple transgenic mouse model of Alzheimer’s disease. J Agric Food Chem 65(24):4970–4979CrossRefGoogle Scholar
- 21.Kattimani VS, Chakravarthi PS, Kanumuru NR, Subbarao VV, Sidharthan A, Kumar TS, Prasad LK (2014) Eggshell derived hydroxyapatite as bone graft substitute in the healing of maxillary cystic bone defects: a preliminary report. J Int Oral Health 6(3):15–19Google Scholar