Journal of Sol-Gel Science and Technology

, Volume 87, Issue 3, pp 600–607 | Cite as

Eggshell derived Se-doped HA nanorods for enhanced antitumor effect and curcumin delivery

  • Yanhua Wang
  • Wencong He
  • Hang Hao
  • Jianxiong Wu
  • Na Qin
Original Paper: Nano-structured materials (particles, fibers, colloids, composites, etc.)


Using natural resources to prepare biomedical materials becomes the focus of current researches. This work presented the Se-doped HA nanorods, which were synthesized using waste eggshell by co-precipitation and hydrothermal route, aiming at the application of the therapy of bone tumor postoperatively. The synthetic products exhibited dispersive nanorods morphology with a length of about 50 nm and a width of 6 nm. And the physicochemical characterization indicated these nanorods with main phase of hydroxyapatite, and they exhibited excellent loading effect for curcumin and slowly stable controlled release fashion in the physiological buffers. 1.38% of the curcumin initially loaded into the nanorods was released into PBS solution over 159 h. Additionally, they exhibited good blood compatibility because of no hemolytic response and cytotoxicity with the healthy whole blood. Together, the Se-doped HA nanorods, not only possess the synthetic advantages such as simple-operation, resource-saving, pollution-free, but also serve as a promising nano-carrier for antitumor drug delivery applied in the onco-therapy of bone tumor and bone regenerative medicine.

Greener synthesis of the HASe nanorods derived from raw eggshell under hydrothermal process. The raw eggshell were hydrothermally treated to fabricate Se-doped HA (HASe) nanorods. The structure and biological properties were detected by physicochemical characterization. Results indicated that the HASe nanorods might form under this hydrothermal condition. The resulting HASe nanorods presented high loading capacity and slow steady release fashion for curcumin, low cellular toxicity and good blood compatibility, and strong anticancer effect on osteosarcoma.


  • A greener synthesis route was developed to fabricate the Se-doped HA nanorods.

  • Se doped HA nanorods showed steadily slow release fashion for curcumin.

  • Se doping into HA nanorods presented better blood compatibility and stronger antitumor effect on osteosarcoma.


Greener synthesis Eggshell Hydroxyapatite Selenium Curcumin Delivery 



We sincerely thank for the help from Dr. Huiyao Xiang from the first college of clinical medical science of China Three Gorges University for the constructive suggestion and data analysis about this study. This work was supported by National Natural Science Foundation of China (grant no. 81602559) and by Youth Science Fund Program of China Three Gorges University (grant no. 1115064).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10971_2018_4765_MOESM1_ESM.doc (2.7 mb)
Supplementary Material


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yanhua Wang
    • 1
  • Wencong He
    • 2
  • Hang Hao
    • 3
  • Jianxiong Wu
    • 1
  • Na Qin
    • 1
  1. 1.Department of MorphologyMedical Science College of China Three Gorges UniversityYichangChina
  2. 2.The First College of Clinical Medical ScienceChina Three Gorges UniversityYichangChina
  3. 3.Advanced Biomaterials and Tissue Engineering CenterHuazhong University of Science and TechnologyWuhanChina

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