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Eggshell derived Se-doped HA nanorods for enhanced antitumor effect and curcumin delivery

  • Yanhua Wang
  • Wencong He
  • Hang Hao
  • Jianxiong Wu
  • Na Qin
Original Paper: Nano-structured materials (particles, fibers, colloids, composites, etc.)
  • 17 Downloads

Abstract

Using natural resources to prepare biomedical materials becomes the focus of current researches. This work presented the Se-doped HA nanorods, which were synthesized using waste eggshell by co-precipitation and hydrothermal route, aiming at the application of the therapy of bone tumor postoperatively. The synthetic products exhibited dispersive nanorods morphology with a length of about 50 nm and a width of 6 nm. And the physicochemical characterization indicated these nanorods with main phase of hydroxyapatite, and they exhibited excellent loading effect for curcumin and slowly stable controlled release fashion in the physiological buffers. 1.38% of the curcumin initially loaded into the nanorods was released into PBS solution over 159 h. Additionally, they exhibited good blood compatibility because of no hemolytic response and cytotoxicity with the healthy whole blood. Together, the Se-doped HA nanorods, not only possess the synthetic advantages such as simple-operation, resource-saving, pollution-free, but also serve as a promising nano-carrier for antitumor drug delivery applied in the onco-therapy of bone tumor and bone regenerative medicine.

Greener synthesis of the HASe nanorods derived from raw eggshell under hydrothermal process. The raw eggshell were hydrothermally treated to fabricate Se-doped HA (HASe) nanorods. The structure and biological properties were detected by physicochemical characterization. Results indicated that the HASe nanorods might form under this hydrothermal condition. The resulting HASe nanorods presented high loading capacity and slow steady release fashion for curcumin, low cellular toxicity and good blood compatibility, and strong anticancer effect on osteosarcoma.

Highlights

  • A greener synthesis route was developed to fabricate the Se-doped HA nanorods.

  • Se doped HA nanorods showed steadily slow release fashion for curcumin.

  • Se doping into HA nanorods presented better blood compatibility and stronger antitumor effect on osteosarcoma.

Keywords

Greener synthesis Eggshell Hydroxyapatite Selenium Curcumin Delivery 

Notes

Acknowledgements

We sincerely thank for the help from Dr. Huiyao Xiang from the first college of clinical medical science of China Three Gorges University for the constructive suggestion and data analysis about this study. This work was supported by National Natural Science Foundation of China (grant no. 81602559) and by Youth Science Fund Program of China Three Gorges University (grant no. 1115064).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10971_2018_4765_MOESM1_ESM.doc (2.7 mb)
Supplementary Material

References

  1. 1.
    Yang BW, Yin JH, Chen Y, Pan SS, Yao HL, Gao YS, Shi JL (2018) 2D-black-phosphorus-reinforced 3D-printed scaffolds: a stepwise countermeasure for osteosarcoma. Adv Mater 30(10):1705611–1705622CrossRefGoogle Scholar
  2. 2.
    Behjati S, Tarpey PS, Haase K, Ye H, Young MD, Alexandrov LB, Farndon SJ, Collord G, Wedge DC, Martincorena I, Cooke SL, Davies H, Mifsud W, Lidgren M, Martin S, Latimer C, Maddison M, Butler AP, Teague JW, Pillay N, Shlien A, McDermott U, Futreal PA, Baumhoer D, Zaikova O, Bjerkehagen B, Myklebost O, Amary MF, Tirabosco R, Van Loo P, Stratton MR, Flanagan AM, Campbell PJ (2017) Recurrent mutation of IGF signalling genes and distinct patterns of genomic rearrangement in osteosarcoma. Nat Commun 8:15936–15943CrossRefGoogle Scholar
  3. 3.
    Kager L, Tamamyan G, Bielack S (2017) Novel insights and therapeutic interventions for pediatric osteosarcoma. Future Oncol 13(4):357–368CrossRefGoogle Scholar
  4. 4.
    Zhou ZF, Sun TW, Chen F, Zuo DQ, Wang HS, Hua YQ, Cai ZD, Tan J (2017) Calcium phosphate-phosphorylated adenosine hybrid microspheres for anti-osteosarcoma drug delivery and osteogenic differentiation. Biomaterials 121:1–14CrossRefGoogle Scholar
  5. 5.
    Zhang Y, Zhang L, Ban Q, Li J, Li CH, Guan YQ (2018) Preparation and characterization of hydroxyapatite nanoparticles carrying insulin and gallic acid for insulin oral delivery. Nanomedicine 14(2):353–364CrossRefGoogle Scholar
  6. 6.
    Khajuria DK, Kumar VB, Gigi D, Gedanken A, Karasik D (2018) Accelerated bone regeneration by nitrogen-doped carbon dots functionalized with hydroxyapatite nanoparticles. ACS Appl Mater Interfaces 10(23):19373–19385CrossRefGoogle Scholar
  7. 7.
    Mancuso E, Bretcanu O, Marshall M, Dalgarno KW (2017) Sensitivity of novel silicate and borate-based glass structures on in vitro bioactivity and degradation behaviour. Ceram Int 43(15):12651–12657CrossRefGoogle Scholar
  8. 8.
    Thomas SC, Sharma H, Rawat P, Verma AK, Leekha A, Kumar V, Tyagi A, Gurjar BS, Iqbal Z, Talegaonkar S (2016) Synergistic anticancer efficacy of bendamustine hydrochloride loaded bioactive Hydroxyapatite nanoparticles: in-vitro, ex-vivo, and in-vivo evaluation. Colloids Surf B Biointerfaces 146:852–860CrossRefGoogle Scholar
  9. 9.
    Bauer IW, Li SP, Han YC, Yuan L, Yin MZ (2008) Internalization of hydroxyapatite nanoparticles in liver cancer cells. J Mater Sci Mater Med 19:1091–1095CrossRefGoogle Scholar
  10. 10.
    Yuan Y, Liu C, Qian J, Wang J, Zhang Y (2010) Size-mediated cytotoxicity and apoptosis of hydroxyapatite nanoparticles in human hepatoma HepG2 cells. Biomaterials 31:730–740CrossRefGoogle Scholar
  11. 11.
    Laurencin D, Almora-Barrios N, de Leeuw NH, Gervais C, Bonhomme C, Mauri F, Chrzanowski W, Knowles JC, Newport RJ, Wong A, Gan Z, Smith ME (2011) Magnesium incorporation into hydroxyapatite. Biomaterials 32:1826–1837CrossRefGoogle Scholar
  12. 12.
    Li Y, Li Q, Zhu S, Luo E, Li J, Feng G, Liao Y, Hu J (2010) The effect of strontium-substituted hydroxyapatite coating on implant fixation in ovariectomized rats. Biomaterials 31:9006–9014CrossRefGoogle Scholar
  13. 13.
    Thian ES, Huang J, Best SM, Barber ZH, Brooks RA, Rushton N, Bonfield W (2006) The response of osteoblasts to nanocrystalline silicon-substituted hydroxyapatite thin films. Biomaterials 27:2692–2698CrossRefGoogle Scholar
  14. 14.
    Gomes S, Nedelec JM, Renaudin G (2012) On the effect of temperature on the insertion of zinc into hydroxyapatite. Acta Biomater 8:1180–1189CrossRefGoogle Scholar
  15. 15.
    Zhang ZH, Wen L, Wu QY, Chen C, Zheng R, Liu Q, Ni JZ, Song GL (2017) Long-term dietary supplementation with selenium-enriched yeast improves cognitive impairment, reverses synaptic deficits and mitigates tau pathology in a triple transgenic mouse model of Alzheimer’s disease. J Agric Food Chem 65(24):4970–4979CrossRefGoogle Scholar
  16. 16.
    Ma J, Wang Y, Zhou L, Zhang S (2013) Preparation and characterization of selenite substituted hydroxyapatite. Mater Sci Eng C Mater Biol Appl 33(1):440–445CrossRefGoogle Scholar
  17. 17.
    Wang Y, Wang J, Hao H, Cai M, Wang S, Ma J, Li Y, Mao C, Zhang S (2016) In vitro and in vivo mechanism of bone tumor inhibition by selenium-doped bone mineral nanoparticles. ACS Nano 10(11):9927–9937CrossRefGoogle Scholar
  18. 18.
    Wang Y, Hao H, Zhang S (2016) Lysozyme loading and release from Se-doped hydroxyapatite nanoparticles. Mater Sci Eng C Mater Biol Appl 61:545–552CrossRefGoogle Scholar
  19. 19.
    Wang Y, Ma J, Zhou L, Chen J, Liu Y, Qiu Z, Zhang S (2012) Dual functional selenium-substituted hydroxyapatite. Interface Focus 2(3):378–386CrossRefGoogle Scholar
  20. 20.
    Wang Y, Hao H, Liu H, Wang Y, Li Y, Yang G, Ma J, Mao C, Zhang S (2015) Selenite-releasing bone mineral nanoparticles retard bone tumor growth and improve healthy tissue functions in vivo. Adv Healthc Mater 4(12):1813–1818CrossRefGoogle Scholar
  21. 21.
    Kattimani VS, Chakravarthi PS, Kanumuru NR, Subbarao VV, Sidharthan A, Kumar TS, Prasad LK (2014) Eggshell derived hydroxyapatite as bone graft substitute in the healing of maxillary cystic bone defects: a preliminary report. J Int Oral Health 6(3):15–19Google Scholar
  22. 22.
    Siva Rama Krishna D, Siddharthan A, Seshadri SK, Sampath Kumar TS (2007) A novel route for synthesis of nanocrystalline hydroxyapatite from eggshell waste. J Mater Sci Mater Med 18(9):1735–1743CrossRefGoogle Scholar
  23. 23.
    Lee SJ, Yoon YS, Lee MH, Oh NS (2007) Nanosized hydroxyapatite powder synthesized from eggshell and phosphoric acid. J Nanosci Nanotechnol 7(11):4061–4064CrossRefGoogle Scholar
  24. 24.
    Prabakaran K, Rajeswari S (2009) Spectroscopic investigations on the synthesis of nano-hydroxyapatite from calcined eggshell by hydrothermal method using cationic surfactant as template. Spectrochim Acta A Mol Biomol Spectrosc 74(5):1127–1134CrossRefGoogle Scholar
  25. 25.
    Kattimani V, Lingamaneni KP, Chakravarthi PS, Kumar TS, Siddharthan A (2016) Eggshell-derived hydroxyapatite: a new era in bone regeneration. J Craniofac Surg 27(1):112–117CrossRefGoogle Scholar
  26. 26.
    Lai W, Chen C, Ren X, Lee IS, Jiang G, Kong X (2016) Hydrothermal fabrication of porous hollow hydroxyapatite microspheres for a drug delivery system. Mater Sci Eng C Mater Biol Appl 62:166–172CrossRefGoogle Scholar
  27. 27.
    Sun J, Zheng X, Li H, Fan D, Song Z, Ma H, Hua X, Hui J (2017) Monodisperse selenium-substituted hydroxyapatite: controllable synthesis and biocompatibility. Mater Sci Eng C Mater Biol Appl 73:596–602CrossRefGoogle Scholar
  28. 28.
    Uskokovic V, Iyer MA, Wu VM (2017) One ion to rule them all: combined antibacterial, osteoinductive and anticancer properties of selenite-incorporated hydroxyapatite. J Mater Chem B 5(7):1430–1445CrossRefGoogle Scholar
  29. 29.
    Kannan S, Rocha JH, Agathopoulos S, Ferreira JM (2007) Fluorine-substituted hydroxyapatite scaffolds hydrothermally grown from aragonitic cuttlefish bones. Acta Biomater 3(2):243–249CrossRefGoogle Scholar
  30. 30.
    Wang X, Li X, Ito A, Watanabe Y, Tsuji NM (2016) Rod-shaped and fluorine-substituted hydroxyapatite free of molecular immunopotentiators stimulates anti-cancer immunity in vivo. Chem Commun 52(44):7078–7081CrossRefGoogle Scholar
  31. 31.
    Szymusiak M, Hu X, Leon Plata PA, Ciupinski P, Wang ZJ, Liu Y (2016) Bioavailability of curcumin and curcumin glucuronide in the central nervous system of mice after oral delivery of nano-curcumin. Int J Pharm 511(1):415–423CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yanhua Wang
    • 1
  • Wencong He
    • 2
  • Hang Hao
    • 3
  • Jianxiong Wu
    • 1
  • Na Qin
    • 1
  1. 1.Department of MorphologyMedical Science College of China Three Gorges UniversityYichangChina
  2. 2.The First College of Clinical Medical ScienceChina Three Gorges UniversityYichangChina
  3. 3.Advanced Biomaterials and Tissue Engineering CenterHuazhong University of Science and TechnologyWuhanChina

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