Journal of Sol-Gel Science and Technology

, Volume 68, Issue 3, pp 447–454 | Cite as

Urease adsorption and activity on magnetite nanoparticles functionalized with monofunctional and bifunctional surface layers

  • Roman P. Pogorilyi
  • Inna V. MelnykEmail author
  • Yuriy L. ZubEmail author
  • Guliam A. Seisenbaeva
  • Vadim G. Kessler
  • Mykola M. Shcherbatyik
  • Aljoša Košak
  • Aleksandra Lobnik
Original Paper


The surface of magnetite nanoparticles was coated with functional polysiloxane layers using reaction of hydrolytic copolycondensation of tetraethoxysilane and 3-aminopropyltriethoxysilane (or N-[3-trimethoxysilylpropyl]ethylendiamine), and also that of tetraethoxysilane, 3-aminopropyltriethoxysilane and methyltriethoxysilane (or n-propyltriethoxysilane). It was shown that these functionalized magnetically controllable particles (about 60–150 nm in size as aggregates), as opposed to magnetite, adsorb urease well from aqueous solutions (up to 1 g/g), and that the level of residual activity of adsorbed layers is up to 84 % in the case of a bifunctional sample. It was established that the activity of immobilized urease is normally gradually reduced during storage of the samples, but in the case of ethylenediamine functional group is not decreased for 45 days. The synthesized samples are promising for use as magnetically directed biocatalysts.


Hydrolytic polycondensation reaction Magnetite Aminogroups surface layer Urease Adsorption Activity 



R. P. P., I. V. M. and Yu. L. Z. would like to thank the State Target Scientific and Technical Program “Nanotechnologies and Nanomaterials” (project and TCPFR “Fundamental Problems of Nanostructural Systems, Nanomaterials, and Nanotechnologies” (project no. 57/12-H) of NAS of Ukraine. V. G. K. and G. A. S. express their gratitude to the Swedish Research Council for support of the project “Molecular Precursors and Molecular Models of Nanoporous Materials” and to the EU FP7 program for support of the EuRARE project.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Roman P. Pogorilyi
    • 1
  • Inna V. Melnyk
    • 1
    Email author
  • Yuriy L. Zub
    • 1
    Email author
  • Guliam A. Seisenbaeva
    • 2
  • Vadim G. Kessler
    • 2
  • Mykola M. Shcherbatyik
    • 3
  • Aljoša Košak
    • 4
  • Aleksandra Lobnik
    • 4
  1. 1.Chuiko Institute of Surface ChemistryNational Academy of Sciences of UkraineKyivUkraine
  2. 2.Deparment of ChemistrySwedish University of Agricultural SciencesUppsalaSweden
  3. 3.Kholodnyi Institute of BotanyNational Academy of Sciences of UkraineKyivUkraine
  4. 4.Faculty of Mechanical Engineering, Centre for Sensor TechnologyUniversity of MariborMariborSlovenia

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