Urease adsorption and activity on magnetite nanoparticles functionalized with monofunctional and bifunctional surface layers
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The surface of magnetite nanoparticles was coated with functional polysiloxane layers using reaction of hydrolytic copolycondensation of tetraethoxysilane and 3-aminopropyltriethoxysilane (or N-[3-trimethoxysilylpropyl]ethylendiamine), and also that of tetraethoxysilane, 3-aminopropyltriethoxysilane and methyltriethoxysilane (or n-propyltriethoxysilane). It was shown that these functionalized magnetically controllable particles (about 60–150 nm in size as aggregates), as opposed to magnetite, adsorb urease well from aqueous solutions (up to 1 g/g), and that the level of residual activity of adsorbed layers is up to 84 % in the case of a bifunctional sample. It was established that the activity of immobilized urease is normally gradually reduced during storage of the samples, but in the case of ethylenediamine functional group is not decreased for 45 days. The synthesized samples are promising for use as magnetically directed biocatalysts.
KeywordsHydrolytic polycondensation reaction Magnetite Aminogroups surface layer Urease Adsorption Activity
R. P. P., I. V. M. and Yu. L. Z. would like to thank the State Target Scientific and Technical Program “Nanotechnologies and Nanomaterials” (project 22.214.171.124) and TCPFR “Fundamental Problems of Nanostructural Systems, Nanomaterials, and Nanotechnologies” (project no. 57/12-H) of NAS of Ukraine. V. G. K. and G. A. S. express their gratitude to the Swedish Research Council for support of the project “Molecular Precursors and Molecular Models of Nanoporous Materials” and to the EU FP7 program for support of the EuRARE project.
- 1.Eltekova NA, Eltekov AY (2000) Sieve effect at the adsorption of albumin and gamma globulin. Russ J Phys Chem 74:1075–1079 (in Russian)Google Scholar
- 7.Brinker CJ, Sheerer GW (1990) Sol–gel science. The physics and chemistry of sol–gel processing. Academic Press, San DiegoGoogle Scholar
- 9.Iler RK (1979) The chemistry of silica: solubility, polymerization, colloid and surface properties, and biochemistry. Wiley, New YorkGoogle Scholar
- 12.Pogorilyi RP, Goncharyk VP, Yurchenko GR et al (2005) Immobilization of urease on functionalized polisyloxane xerogels and hydrogels obtained by sol–gel method. Quest Chem Chem Technol 6:103–106 (in Ukraininan)Google Scholar
- 20.Lurie YY (1984) Analytical chemistry of industrial wastewater. Chemistry, Moscow (in Russian)Google Scholar
- 22.Finn L, Slinyakova I (1975) The structure and thermal decomposition of the organopolysiloxane xerogels shown by IR spectroscopy. Colloid J 37:723–729 (in Russian)Google Scholar
- 23.Lin-Vien D, Colthup NB, Fateley WG, Grasselli JG (1991) The handbook of infrared and Raman characteristic frequencies of organic molecules. Academic Press, LondonGoogle Scholar
- 26.Pogorilyi RP, Goncharyk VP, Kozhara LI, Zub YL (2009) The influence on the activity of the adsorbed urease of structural and adsorption characteristics of polisyloxane matrices containing surface layer with 3-aminopropyl groups. Surface 1(16):35–45 (in Ukrainian)Google Scholar
- 28.Pogorilyi RP, Honcharyk VP, Melnyk IV, Zub YuL (2008) In: Innocenzi P, Zub Yu, Kessler V (eds) Sol–gel methods for materials processing, XII. Springer, DordrechtGoogle Scholar