Journal of Sol-Gel Science and Technology

, Volume 50, Issue 1, pp 111–118

Effect of organic and inorganic acids on chitosan/glycerophosphate thermosensitive hydrogel

  • Qing Sheng Zhao
  • Xiao Jie Cheng
  • Qiu Xia Ji
  • Chuan Zhen Kang
  • Xi Guang Chen
Original Paper

DOI: 10.1007/s10971-008-1891-0

Cite this article as:
Zhao, Q.S., Cheng, X.J., Ji, Q.X. et al. J Sol-Gel Sci Technol (2009) 50: 111. doi:10.1007/s10971-008-1891-0

Abstract

The influence of organic and inorganic acids on chitosan/glycerophosphate (CS/GP) hydrogel has been investigated by dissolving chitosan in different acids. The results of gelation showed that all of the chitosan dissolved in monovalent acid solutions (i.e., formic, acetic, propionic, butyric, isobutyric, lactic, nitric, hydrochloric, and chloroacetic acid), when neutralized by GP solution, could transform into hydrogel after 2–5 min at 37 °C, while those dissolved in multivalent acids failed in gel formation. The inner structures of CS/GP hydrogels prepared with monovalent acids depended on the ionic strength and chain length of acids. Morphological examination by scanning electron microphotography demonstrated that large pores occurred during the gel-forming process, and the aperture size was also related to different acids. The cytotoxicity studies indicated that CS/GP systems prepared by dissolving chitosan in tested acids except chloroacetic acid were nontoxic to mouse embryonic fibroblasts and Hela cells.

Keywords

Chitosan Glycerophosphate Hydrogel Cytotoxicity 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Qing Sheng Zhao
    • 1
  • Xiao Jie Cheng
    • 1
  • Qiu Xia Ji
    • 2
  • Chuan Zhen Kang
    • 1
  • Xi Guang Chen
    • 1
  1. 1.College of Marine Life ScienceOcean University of ChinaQingdaoPeople’s Republic of China
  2. 2.The Affiliated Hospital of Medical College Qingdao UniversityQingdaoPeople’s Republic of China

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