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Journal of Sol-Gel Science and Technology

, Volume 45, Issue 2, pp 157–163 | Cite as

Structural characteristics and cyclic voltammetric behaviour of Sonogel-Carbon tyrosinase biosensors. A detailed comparative study of three immobilization matrixes

  • Mohammed ElKaoutit
  • Ignacio Naranjo-Rodríguez
  • Khalid Riffi Temsamani
  • Manuel Domínguez
  • José Luis Hidalgo-Hidalgo-de-Cisneros
Original Paper

Abstract

Structural characteristics an cyclic voltammetry of three amperommetric biosensors based on immobilization of tyrosinase on a Sonogel-Carbon electrode for detection of phenols are described. Cyclic voltammetry was applied to study the electrochemical behaviour of the electrode and the electrochemical reaction on the electrode surface. Scanning electron microscopy, X-ray energy dispersive spectroscopy and atomic force microscopy were used for the structure characterization of the electrode surface, enzyme film and polymers coatings. The influence of additive-protective polymers, such as polyethylene glycol and perfluorinated-Nafion ion-exchanger on the surface of the biosensor were explored.

Keywords

Biosensor Sonogel-Carbon Tyrosinase Catechol Cyclic voltammetry Scanning electron microscopy Atomic force microscopy X-ray energy dispersive spectroscopy 

Notes

Acknowledgements

The authors are grateful to FEDER (Fondo Europeo de Desarrollo Económico y Regional) and the Ministerio de Educación y Ciencia of Spain (Project CTQ2004-03708/BQU) and to the Junta de Andalucía for financial support, and Agencia Española de Cooperación Internacional (AECI) through a grant to Mohammed ElKaoutit.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Mohammed ElKaoutit
    • 1
  • Ignacio Naranjo-Rodríguez
    • 1
  • Khalid Riffi Temsamani
    • 2
  • Manuel Domínguez
    • 3
  • José Luis Hidalgo-Hidalgo-de-Cisneros
    • 1
  1. 1.Departamento de Química Analítica, Facultad de CienciasUniversidad de CádizPuerto RealSpain
  2. 2.Departement de Chimie, Equipe de Recherche Electrochimie et Systèmes Interfaciaux, Faculté des Siences de TétouanUniversité Abdelmalek EssaâdiM’Hannech IIMorocco
  3. 3.Departamento de Física de la Materia Condensada, Facultad de CienciasUniversidad de CádizPuerto RealSpain

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