Crystal structures of Mycobacterial MeaB and MMAA-like GTPases

  • Thomas E. Edwards
  • Loren Baugh
  • Jameson Bullen
  • Ruth O. Baydo
  • Pam Witte
  • Kaitlin Thompkins
  • Isabelle Q. H. Phan
  • Jan Abendroth
  • Matthew C. Clifton
  • Banumathi Sankaran
  • Wesley C. Van Voorhis
  • Peter J. Myler
  • Bart L. Staker
  • Christoph Grundner
  • Donald D. Lorimer
Article

Abstract

The methylmalonyl Co-A mutase-associated GTPase MeaB from Methylobacterium extorquens is involved in glyoxylate regulation and required for growth. In humans, mutations in the homolog methylmalonic aciduria associated protein (MMAA) cause methylmalonic aciduria, which is often fatal. The central role of MeaB from bacteria to humans suggests that MeaB is also important in other, pathogenic bacteria such as Mycobacteriumtuberculosis. However, the identity of the mycobacterial MeaB homolog is presently unclear. Here, we identify the M. tuberculosis protein Rv1496 and its homologs in M. smegmatis and M. thermoresistibile as MeaB. The crystal structures of all three homologs are highly similar to MeaB and MMAA structures and reveal a characteristic three-domain homodimer with GDP bound in the G domain active site. A structure of Rv1496 obtained from a crystal grown in the presence of GTP exhibited electron density for GDP, suggesting GTPase activity. These structures identify the mycobacterial MeaB and provide a structural framework for therapeutic targeting of M. tuberculosis MeaB.

Keywords

MeaB Metallochaperone Methylmalonic aciduria associated protein MMAA Mycobacterium Ras-like GTPase Structural genomics Tuberculosis 

Abbreviations

ALS

Advanced Light Source

APF

Atomic property force

ASU

Asymmetric unit

GTP

Guanosine 5′ triphosphate

IMAC

Immobilized metal ion affinity chromatography

MCM

Methylmalonyl-CoA-mutase

MMAA

Methylmalonyl aciduria associated protein A

PDB

Protein Data Bank

SBM

Sleeping beauty mutase

SSGCID

Seattle Structural Genomics Center for Infectious Disease

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Thomas E. Edwards
    • 1
    • 2
  • Loren Baugh
    • 3
  • Jameson Bullen
    • 1
    • 2
  • Ruth O. Baydo
    • 1
    • 2
  • Pam Witte
    • 1
    • 2
  • Kaitlin Thompkins
    • 3
  • Isabelle Q. H. Phan
    • 3
  • Jan Abendroth
    • 1
    • 2
  • Matthew C. Clifton
    • 1
    • 2
  • Banumathi Sankaran
    • 4
  • Wesley C. Van Voorhis
    • 5
  • Peter J. Myler
    • 3
    • 6
    • 7
  • Bart L. Staker
    • 3
  • Christoph Grundner
    • 3
  • Donald D. Lorimer
    • 1
    • 2
  1. 1.Beryllium, Seattle Structural Genomics Center for Infectious Disease (SSGCID)Bainbridge IslandUSA
  2. 2.Beryllium, Seattle Structural Genomics Center for Infectious Disease (SSGCID)BedfordUSA
  3. 3.Seattle Biomedical Research InstituteSeattle Structural Genomics Center for Infectious Disease (SSGCID)SeattleUSA
  4. 4.Berkeley Center for Structural BiologyErnest Orlando Lawrence Berkeley National LaboratoryBerkeleyUSA
  5. 5.Departments of Medicine, Microbiology, and Global HealthUniversity of Washington, Seattle Structural Genomics Center for Infectious Disease (SSGCID)SeattleUSA
  6. 6.Department of Medical Education and Biomedical InformationUniversity of WashingtonSeattleUSA
  7. 7.Department of Global HealthUniversity of WashingtonSeattleUSA

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