Journal of Structural and Functional Genomics

, Volume 15, Issue 4, pp 209–214 | Cite as

Solution NMR structures of immunoglobulin-like domains 7 and 12 from obscurin-like protein 1 contribute to the structural coverage of the human cancer protein interaction network

  • Surya V. S. R. K. Pulavarti
  • Yuanpeng J. Huang
  • Kari Pederson
  • Thomas B. Acton
  • Rong Xiao
  • John K. Everett
  • James H. Prestegard
  • Gaetano T. Montelione
  • Thomas Szyperski


High-quality solution NMR structures of immunoglobulin-like domains 7 and 12 from human obscurin-like protein 1 were solved. The two domains share 30 % sequence identity and their structures are, as expected, rather similar. The new structures contribute to structural coverage of human cancer associated proteins. Mutations of Arg 812 in domain 7 cause the rare 3-M syndrome, and this site is located in a surface area predicted to be involved in protein–protein interactions.


PF07679 Obscurin-like protein 1 Cytoskeletal adapter Ig-like domain Structural genomics 





Coiled-coil domain containing protein 8


4,4-Dimethyl-4-silapentane-1-sulfonate sodium salt




Fbox and WD repeat containing protein 8


Human Cancer Pathway Interaction Network




2-(N-morpholino)ethanesulfonic acid


Mitogen-activated protein kinase


Northeast Structural Genomics Consortium


Nuclear Overhauser enhancement spectroscopy


Obscurin-like protein 1


Protein Data Bank


Residual dipolar coupling


Root mean square deviation


Toll-like receptor





This work was supported by the National Institutes of Health, grant number: U54 GM094597 (T.S., J.H.P. and G.T.M.).

Supplementary material

10969_2014_9185_MOESM1_ESM.pdf (710 kb)
Supplementary material 1 (PDF 710 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Surya V. S. R. K. Pulavarti
    • 1
  • Yuanpeng J. Huang
    • 3
  • Kari Pederson
    • 2
  • Thomas B. Acton
    • 3
  • Rong Xiao
    • 3
  • John K. Everett
    • 3
  • James H. Prestegard
    • 2
  • Gaetano T. Montelione
    • 3
    • 4
  • Thomas Szyperski
    • 1
  1. 1.Department of ChemistryThe State University of New York at Buffalo and Northeast Structural Genomics ConsortiumBuffaloUSA
  2. 2.Complex Carbohydrate Research Center and Northeast Structural Genomics ConsortiumUniversity of GeorgiaAthensUSA
  3. 3.Center of Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, RutgersThe State University of New Jersey and Northeast Structural Genomics ConsortiumPiscatawayUSA
  4. 4.Department of Biochemistry and Molecular BiologyRobert Wood Johnson Medical School, UMDNJPiscatawayUSA

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