Journal of Structural and Functional Genomics

, Volume 13, Issue 3, pp 171–176 | Cite as

Solution NMR structure of Alr2454 from Nostoc sp. PCC 7120, the first structural representative of Pfam domain family PF11267

  • James M. Aramini
  • Donald Petrey
  • Dong Yup Lee
  • Haleema Janjua
  • Rong Xiao
  • Thomas B. Acton
  • John K. Everett
  • Gaetano T. Montelione


Protein domain family PF11267 (DUF3067) is a family of proteins of unknown function found in both bacteria and eukaryotes. Here we present the solution NMR structure of the 102-residue Alr2454 protein from Nostoc sp. PCC 7120, which constitutes the first structural representative from this conserved protein domain family. The structure of Nostoc sp. Alr2454 adopts a novel protein fold.


Alr2454 protein DUF3067 PF11267 Protein Structure Initiative Solution NMR structure Structural genomics 



2,2-Dimethyl-2-silapentane-5-sulfonic acid




Heteronuclear single quantum coherence


Isopropyl β-D-1-thiogalactopyranoside


Nuclear Overhauser effect


Northeast Structural Genomics Consortium


Protein Data Bank





The authors thank Roberto Tejero, Barry Honig, Bomina Yu, Colleen Ciccosanti, and G.V.T. Swapna for helpful discussions. This research was supported by National Institute of General Medical Sciences Protein Structure Initiative (PSI-Biology) program grants U54-GM074958 and U54-GM094597.

Supplementary material

10969_2012_9135_MOESM1_ESM.pdf (928 kb)
Supplementary material 1 (PDF 927 kb)


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • James M. Aramini
    • 1
  • Donald Petrey
    • 2
  • Dong Yup Lee
    • 1
  • Haleema Janjua
    • 1
  • Rong Xiao
    • 1
  • Thomas B. Acton
    • 1
  • John K. Everett
    • 1
  • Gaetano T. Montelione
    • 1
    • 3
  1. 1.Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, and the Northeast Structural Genomics ConsortiumRutgers, The State University of New JerseyPiscatawayUSA
  2. 2.Department of Biochemistry and Medical Biophysics, Center for Computational Biology and Bioinformatics, and the Northeast Structural Genomics ConsortiumColumbia UniversityNew YorkUSA
  3. 3.Department of Biochemistry, Robert Wood Johnson Medical SchoolUniversity of Medicine and Dentistry of New JerseyPiscatawayUSA

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