Journal of Structural and Functional Genomics

, Volume 13, Issue 3, pp 171–176 | Cite as

Solution NMR structure of Alr2454 from Nostoc sp. PCC 7120, the first structural representative of Pfam domain family PF11267

  • James M. Aramini
  • Donald Petrey
  • Dong Yup Lee
  • Haleema Janjua
  • Rong Xiao
  • Thomas B. Acton
  • John K. Everett
  • Gaetano T. Montelione
Article

Abstract

Protein domain family PF11267 (DUF3067) is a family of proteins of unknown function found in both bacteria and eukaryotes. Here we present the solution NMR structure of the 102-residue Alr2454 protein from Nostoc sp. PCC 7120, which constitutes the first structural representative from this conserved protein domain family. The structure of Nostoc sp. Alr2454 adopts a novel protein fold.

Keywords

Alr2454 protein DUF3067 PF11267 Protein Structure Initiative Solution NMR structure Structural genomics 

Abbreviations

DSS

2,2-Dimethyl-2-silapentane-5-sulfonic acid

DTT

Dithiothreitol

HSQC

Heteronuclear single quantum coherence

IPTG

Isopropyl β-D-1-thiogalactopyranoside

NOE

Nuclear Overhauser effect

NESG

Northeast Structural Genomics Consortium

PDB

Protein Data Bank

RMSD

Root-mean-square-deviation

Supplementary material

10969_2012_9135_MOESM1_ESM.pdf (928 kb)
Supplementary material 1 (PDF 927 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • James M. Aramini
    • 1
  • Donald Petrey
    • 2
  • Dong Yup Lee
    • 1
  • Haleema Janjua
    • 1
  • Rong Xiao
    • 1
  • Thomas B. Acton
    • 1
  • John K. Everett
    • 1
  • Gaetano T. Montelione
    • 1
    • 3
  1. 1.Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, and the Northeast Structural Genomics ConsortiumRutgers, The State University of New JerseyPiscatawayUSA
  2. 2.Department of Biochemistry and Medical Biophysics, Center for Computational Biology and Bioinformatics, and the Northeast Structural Genomics ConsortiumColumbia UniversityNew YorkUSA
  3. 3.Department of Biochemistry, Robert Wood Johnson Medical SchoolUniversity of Medicine and Dentistry of New JerseyPiscatawayUSA

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