Journal of Structural and Functional Genomics

, Volume 13, Issue 3, pp 163–170 | Cite as

Three structural representatives of the PF06855 protein domain family from Staphyloccocus aureus and Bacillus subtilis have SAM domain-like folds and different functions

  • G. V. T. Swapna
  • Paolo Rossi
  • Alexander F. Montelione
  • Jordi Benach
  • Bomina Yu
  • Mariam Abashidze
  • Jayaraman Seetharaman
  • Rong Xiao
  • Thomas B. Acton
  • Liang Tong
  • Gaetano T. Montelione
Article
  • 97 Downloads

Abstract

Protein domain family PF06855 (DUF1250) is a family of small domains of unknown function found only in bacteria, and mostly in the order Bacillales and Lactobacillales. Here we describe the solution NMR or X-ray crystal structures of three representatives of this domain family, MW0776 and MW1311 from Staphyloccocus aureus and yozE from Bacillus subtilis. All three proteins adopt a four-helix motif similar to sterile alpha motif (SAM) domains. Phylogenetic analysis classifies MW1311 and yozE as functionally equivalent proteins of the UPF0346 family of unknown function, but excludes MW0776, which likely has a different biological function. Our structural characterization of the three domains supports this separation of function. The structures of MW0776, MW1311, and yozE constitute the first structural representatives from this protein domain family.

Keywords

yozE MW0776 MW1311 UPF0345 DUF1250 PF06855 SAM domain X-ray crystal structure Solution NMR Structural genomics 

Abbreviations

NESG

Northeast Structural Genomics Consortium

PDB

Protein Data Bank

rmsd

Root-mean-square-deviation

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • G. V. T. Swapna
    • 1
    • 2
    • 3
  • Paolo Rossi
    • 1
    • 2
    • 3
  • Alexander F. Montelione
    • 1
    • 2
    • 3
  • Jordi Benach
    • 5
    • 6
  • Bomina Yu
    • 5
    • 6
  • Mariam Abashidze
    • 5
    • 6
  • Jayaraman Seetharaman
    • 5
    • 6
  • Rong Xiao
    • 1
    • 2
    • 3
  • Thomas B. Acton
    • 1
    • 2
    • 3
  • Liang Tong
    • 5
    • 6
  • Gaetano T. Montelione
    • 1
    • 2
    • 3
    • 4
  1. 1.Center for Advanced Biotechnology and MedicineThe State University of New JerseyPiscatawayUSA
  2. 2.Department of Molecular Biology and BiochemistryThe State University of New JerseyPiscatawayUSA
  3. 3.Northeast Structural Genomics Consortium, RutgersThe State University of New JerseyPiscatawayUSA
  4. 4.Robert Wood Johnson Medical School, UMDNJPiscatawayUSA
  5. 5.Department of Biological SciencesColumbia UniversityNew YorkUSA
  6. 6.Northeast Structural Genomics ConsortiumColumbia UniversityNew YorkUSA

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