The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins
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The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.
KeywordsMembrane proteins Structural genomics High throughput NMR X-ray
We thank past and present colleagues who have participated in the development of the Protein Production Facility: Brandon Hillerich, Brian Kloss, Renato Bruni, Arianne Morrison, Patricia Rodriguez, Amanda Meyer, Jeff Bonanno, Zsolt Zolnai, Michael Weiner, Reinhard Grisshammer, and Gunnar von Heijne. This work was supported in part by a Cooperative Agreement from the NIGMS Protein Structure Initiative, U54GM075026.
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