Journal of Structural and Functional Genomics

, Volume 11, Issue 3, pp 191–199 | Cite as

The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins

  • James Love
  • Filippo Mancia
  • Lawrence Shapiro
  • Marco Punta
  • Burkhard Rost
  • Mark Girvin
  • Da-Neng Wang
  • Ming Zhou
  • John F. Hunt
  • Thomas Szyperski
  • Eric Gouaux
  • Roderick MacKinnon
  • Ann McDermott
  • Barry Honig
  • Masayori Inouye
  • Gaetano Montelione
  • Wayne A. Hendrickson
Article

Abstract

The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.

Keywords

Membrane proteins Structural genomics High throughput NMR X-ray 

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • James Love
    • 1
  • Filippo Mancia
    • 2
  • Lawrence Shapiro
    • 3
  • Marco Punta
    • 3
  • Burkhard Rost
    • 3
  • Mark Girvin
    • 4
  • Da-Neng Wang
    • 5
  • Ming Zhou
    • 2
  • John F. Hunt
    • 6
  • Thomas Szyperski
    • 7
  • Eric Gouaux
    • 8
  • Roderick MacKinnon
    • 9
  • Ann McDermott
    • 10
  • Barry Honig
    • 11
  • Masayori Inouye
    • 12
  • Gaetano Montelione
    • 13
  • Wayne A. Hendrickson
    • 11
  1. 1.NYCOMPS Core LaboratoryNew York Structural Biology CenterNew YorkUSA
  2. 2.Department of Physiology and Cellular BiophysicsColumbia UniversityNew YorkUSA
  3. 3.Department of Biochemistry and Molecular BiophysicsColumbia UniversityNew YorkUSA
  4. 4.Department of BiochemistryAlbert Einstein College of MedicineBronx, New YorkUSA
  5. 5.Skirball Institute of Biomolecular MedicineNew York University Medical CenterNew YorkUSA
  6. 6.Department of Biological SciencesColumbia UniversityNew YorkUSA
  7. 7.Department of ChemistryUniversity of BuffaloBuffaloUSA
  8. 8.Howard Hughes Medical Institute, The Vollum InstituteOregon Health & Science UniversityPortlandUSA
  9. 9.Howard Hughes Medical InstituteThe Rockefeller UniversityNew YorkUSA
  10. 10.Department of ChemistryColumbia UniversityNew YorkUSA
  11. 11.Howard Hughes Medical Institute, Department of Biochemistry and Molecular BiophysicsColumbia UniversityNew YorkUSA
  12. 12.Department of Molecular Biology and BiochemistryRutgers, The State University of New JerseyPiscatawayUSA
  13. 13.Department of BiochemistryUMDNJPiscatawayUSA

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