The E. coli single protein production system for production and structural analysis of membrane proteins

  • Lili Mao
  • S. Thangminlal Vaiphei
  • Tsutomu Shimazu
  • William M. Schneider
  • Yuefeng Tang
  • Rajeswari Mani
  • Monica J. Roth
  • Gaetano T. Montelione
  • Masayori Inouye
Article

Abstract

At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.

Keywords

SPP Membrane protein NMR 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Lili Mao
    • 1
    • 2
    • 3
  • S. Thangminlal Vaiphei
    • 1
    • 2
    • 3
  • Tsutomu Shimazu
    • 1
    • 2
    • 3
  • William M. Schneider
    • 4
  • Yuefeng Tang
    • 2
    • 3
    • 5
    • 6
  • Rajeswari Mani
    • 2
    • 3
    • 5
    • 6
  • Monica J. Roth
    • 4
  • Gaetano T. Montelione
    • 2
    • 3
    • 4
    • 5
    • 6
  • Masayori Inouye
    • 1
    • 2
    • 3
  1. 1.Center for Advanced Technology and MedicineUMDNJ-Robert Wood Johnson Medical SchoolPiscatawayUSA
  2. 2.New York Center on Membrane Protein StructureRutgers, The State University of New JerseyPiscatawayUSA
  3. 3.Northeast Structural Genomics ConsortiumRutgers, The State University of New JerseyPiscatawayUSA
  4. 4.Department of BiochemistryUMDNJ-Robert Wood Johnson Medical SchoolPiscatawayUSA
  5. 5.Center for Advanced Biotechnology and MedicineRutgers, The State University of New JerseyPiscatawayUSA
  6. 6.Department of Molecular Biology and BiochemistryRutgers, The State University of New JerseyPiscatawayUSA

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