The E. coli single protein production system for production and structural analysis of membrane proteins
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At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.
KeywordsSPP Membrane protein NMR
- 1.Mao L, Tang Y, Vaiphei ST, Shimazu T, Kim S, Mani R, Fakhoury E, White E, Montelione GT, Inouye M (2009) Production of membrane proteins for NMR Studies using the condensed single protein production (cSPP) system. J Struct Funct Genomics (in press)Google Scholar
- 3.Schneider WM, Tang Y, Vaiphei ST, Mao L, Shen Y, Raman S, Baker D, Inouye M, Roth MJ, Montelione GT (2009) Efficient production of perdeuterated soluble and membrane proteins for NMR studies. Nat Methods (Submitted)Google Scholar