The E. coli single protein production system for production and structural analysis of membrane proteins
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At present, only 0.9% of PDB-deposited structures are of membrane proteins in spite of the fact that membrane proteins constitute approximately 30% of total proteins in most genomes from bacteria to humans. Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks.
KeywordsSPP Membrane protein NMR
This work was supported by the National Institutes of Health Grants U54 GM074958 (G.T.M. and M. I.), U54 GM75026 (G.T.M. and M. I.), and 1R01 GM085449 (M.I.), and partially by a research fund from Takara-Bio., Inc.
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