Functional insights from structural genomics

  • Farhad Forouhar
  • Alexandre Kuzin
  • Jayaraman Seetharaman
  • Insun Lee
  • Weihong Zhou
  • Mariam Abashidze
  • Yang Chen
  • Wei Yong
  • Haleema Janjua
  • Yingyi Fang
  • Dongyan Wang
  • Kellie Cunningham
  • Rong Xiao
  • Thomas B. Acton
  • Eran Pichersky
  • Daniel F. Klessig
  • Carl W. Porter
  • Gaetano T. Montelione
  • Liang Tong
Original Paper

Abstract

Structural genomics efforts have produced structural information, either directly or by modeling, for thousands of proteins over the past few years. While many of these proteins have known functions, a large percentage of them have not been characterized at the functional level. The structural information has provided valuable functional insights on some of these proteins, through careful structural analyses, serendipity, and structure-guided functional screening. Some of the success stories based on structures solved at the Northeast Structural Genomics Consortium (NESG) are reported here. These include a novel methyl salicylate esterase with important role in plant innate immunity, a novel RNA methyltransferase (H. influenzae yggJ (HI0303)), a novel spermidine/spermine N-acetyltransferase (B. subtilis PaiA), a novel methyltransferase or AdoMet binding protein (A. fulgidus AF_0241), an ATP:cob(I)alamin adenosyltransferase (B. subtilis YvqK), a novel carboxysome pore (E. coli EutN), a proline racemase homolog with a disrupted active site (B. melitensis BME11586), an FMN-dependent enzyme (S. pneumoniae SP_1951), and a 12-stranded β-barrel with a novel fold (V. parahaemolyticus VPA1032).

Keywords

Methyltransferase Spermine Acetyltransferase 

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Farhad Forouhar
    • 1
  • Alexandre Kuzin
    • 1
  • Jayaraman Seetharaman
    • 1
  • Insun Lee
    • 1
  • Weihong Zhou
    • 1
  • Mariam Abashidze
    • 1
  • Yang Chen
    • 1
  • Wei Yong
    • 1
  • Haleema Janjua
    • 2
  • Yingyi Fang
    • 2
  • Dongyan Wang
    • 2
  • Kellie Cunningham
    • 2
  • Rong Xiao
    • 2
  • Thomas B. Acton
    • 2
  • Eran Pichersky
    • 3
  • Daniel F. Klessig
    • 4
  • Carl W. Porter
    • 5
  • Gaetano T. Montelione
    • 2
  • Liang Tong
    • 1
  1. 1.Department of Biological Sciences, Northeast Structural Genomics ConsortiumColumbia UniversityNew YorkUSA
  2. 2.Center for Advanced Biotechnology and Medicine, Northeast Structural Genomics ConsortiumRutgers UniversityPiscatawayUSA
  3. 3.Department of Molecular, Cellular and Developmental BiologyUniversity of MichiganAnn ArborUSA
  4. 4.Boyce Thompson Institute for Plant ResearchIthacaUSA
  5. 5.Department of Pharmacology and TherapeuticsRoswell Park Cancer InstituteBuffaloUSA

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