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The solution structure of the core of mesoderm development (MESD), a chaperone for members of the LDLR-family

  • Christian Köhler
  • Olav M. Andersen
  • Annette Diehl
  • Gerd Krause
  • Peter Schmieder
  • Hartmut Oschkinat
Original Paper

Abstract

Mesoderm development (MESD) is a 224 amino acid mouse protein that acts as a molecular chaperone for receptors of the low-density lipoprotein receptor (LDLR) family. By recording 15N-HSQC-NMR spectra of six different MESD constructs, we could determine a highly structured core region corresponding to residues 104-177. Here we firstly present the solution structure of this highly conserved core of MESD. It shows a four-stranded anti-parallel β-sheet and two α-helices situated on one side of the sheet. Although described in the literature as structurally homologues to ferredoxins, the connectivity of secondary structure elements is different in the MESD fold. A structural comparison to entries of the PDB reveals a frequent domain with low sequence homology annotated as HMA and P-II domains in Pfam.

Keywords

Boca Ferredoxin-like-fold LDLR-family MESD NMR-structure-determination WNT-signalling 

Notes

Acknowledgements

We would like to thank J. Herz (Department of Molecular Genetics, University Of Texas Southwestern Medical Center, Dallas, Texas, USA) who provided us the DNA template for MESD.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Christian Köhler
    • 1
  • Olav M. Andersen
    • 2
  • Annette Diehl
    • 1
  • Gerd Krause
    • 1
  • Peter Schmieder
    • 1
  • Hartmut Oschkinat
    • 1
  1. 1.Department of NMR-Supported Structural BiologyLeibniz-Institut für Molekulare PharmakologieBerlinGermany
  2. 2.Department of Molecular Cardiovascular ResearchMax-Delbrueck-Center for Molecular MedicineBerlinGermany

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