Journal of Structural and Functional Genomics

, Volume 6, Issue 2–3, pp 225–232 | Cite as

New York-Structural GenomiX Research Consortium (NYSGXRC): A Large Scale Center for the Protein Structure Initiative

  • Jeffrey B. Bonanno
  • Steven C. Almo
  • Anne Bresnick
  • Mark R. Chance
  • Andras Fiser
  • S. Swaminathan
  • J. Jiang
  • F. William Studier
  • Lawrence Shapiro
  • Christopher D. Lima
  • Theresa M. Gaasterland
  • Andrej Sali
  • Kevin Bain
  • Ingeborg Feil
  • Xia Gao
  • Don Lorimer
  • Aurora Ramos
  • J. Michael Sauder
  • Steven R. Wasserman
  • Spencer Emtage
  • Kevin L. D’Amico
  • Stephen K. Burley
Article

Abstract

Structural GenomiX, Inc. (SGX), four New York area institutions, and two University of California schools have formed the New York Structural GenomiX Research Consortium (NYSGXRC), an industrial/academic Research Consortium that exploits individual core competencies to support all aspects of the NIH-NIGMS funded Protein Structure Initiative (PSI), including protein family classification and target selection, generation of protein for biophysical analyses, sample preparation for structural studies, structure determination and analyses, and dissemination of results. At the end of the PSI Pilot Study Phase (PSI-1), the NYSGXRC will be capable of producing 100–200 experimentally determined protein structures annually. All Consortium activities can be scaled to increase production capacity significantly during the Production Phase of the PSI (PSI-2). The Consortium utilizes both centralized and de-centralized production teams with clearly defined deliverables and hand-off procedures that are supported by a web-based target/sample tracking system (SGX Laboratory Information Data Management System, LIMS, and NYSGXRC Internal Consortium Experimental Database, ICE-DB). Consortium management is provided by an Executive Committee, which is composed of the PI and all Co-PIs. Progress to date is tracked on a publicly available Consortium web site (http://www.nysgxrc.org) and all DNA/protein reagents and experimental protocols are distributed freely from the New York City Area institutions. In addition to meeting the requirements of the Pilot Study Phase and preparing for the Production Phase of the PSI, the NYSGXRC aims to develop modular technologies that are transferable to structural biology laboratories in both academe and industry. The NYSGXRC PI and Co-PIs intend the PSI to have a transforming effect on the disciplines of X-ray crystallography and NMR spectroscopy of biological macromolecules. Working with other PSI-funded Centers, the NYSGXRC seeks to create the structural biology laboratory of the future. Herein, we present an overview of the organization of the NYSGXRC and describe progress toward development of a high-throughput Gene→Structure platform. An analysis of current and projected consortium metrics reflects progress to date and delineates opportunities for further technology development.

Key words

crystallography NYSGXRC protein structure initiative structural genomics 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Jeffrey B. Bonanno
    • 1
  • Steven C. Almo
    • 2
  • Anne Bresnick
    • 2
  • Mark R. Chance
    • 2
  • Andras Fiser
    • 2
  • S. Swaminathan
    • 3
  • J. Jiang
    • 3
  • F. William Studier
    • 3
  • Lawrence Shapiro
    • 4
  • Christopher D. Lima
    • 5
  • Theresa M. Gaasterland
    • 6
  • Andrej Sali
    • 7
  • Kevin Bain
    • 1
  • Ingeborg Feil
    • 1
  • Xia Gao
    • 1
  • Don Lorimer
    • 1
  • Aurora Ramos
    • 1
  • J. Michael Sauder
    • 1
  • Steven R. Wasserman
    • 1
  • Spencer Emtage
    • 1
  • Kevin L. D’Amico
    • 1
  • Stephen K. Burley
    • 1
  1. 1.Structural GenomiX, Inc.San DiegoUSA
  2. 2.Albert Einstein College of MedicineBronxUSA
  3. 3.Department of BiologyBrookhaven National LaboratoryUptonUSA
  4. 4.Department BiochemistryColumbia UniversityNew YorkUSA
  5. 5.Structural Biology ProgramSloan-Kettering InstituteNew YorkUSA
  6. 6.University of CaliforniaSan DiegoUSA
  7. 7.University of CaliforniaSan FranciscoUSA

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