Protein Production and Crystallization at the Joint Center for Structural Genomics

  • Scott A. LesleyEmail author
  • Ian A. Wilson


By definition, structural genomics centers must be able to address a large number of diverse protein targets. The methods developed should permit parallel and cost-effective processing while allowing for the diverse nature of proteins. Our approach to this problem is a multi-tiered effort where targets are characterized and categorized by behavior and processed in parallel by appropriate methods. The Joint Center for Structural Genomics (JCSG) has applied this tactic to create a fully integrated and scaleable structure determination pipeline. Highlights of the development of the current pipeline for protein production and crystallization are presented here.

Key words

high throughput automated protein production robotic crystallization salvage pathways protein characterization 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Lesley, S.A., Kuhn, P., Godzik, A., Deacon, A.M., Mathews, I., Kreusch, A., Spraggon, G., Klock, H.E., McMullan, D., Shin, T., Vincent, J., Robb, A., Brinen, L.S., Miller, M.D., McPhillips, T.M., Miller, M.A., Scheibe, D., Canaves, J.M., Guda, C., Jaroszewski, L., Selby, T.L., Elsliger, M.A., Wooley, J., Taylor, S.S., Hodgson, K.O., Wilson, I.A., Schultz, P.G., Stevens, R.C. 2002Proc. Natl. Acad. Sci. U.S.A.991166411669CrossRefPubMedGoogle Scholar
  2. 2.
    DiDonato, M., Deacon, A.M., Klock, H.E., McMullan, D., Lesley, S.A. 2004J. Struct. Funct. Genom.5133146CrossRefGoogle Scholar
  3. 3.
    Lesley, S.A. 2001Protein Expr. Purif.22159164CrossRefPubMedGoogle Scholar
  4. 4.
    Stevens, R.C. 2000Curr. Opin. Struct. Biol.10558563CrossRefPubMedGoogle Scholar
  5. 5.
    Abola, E., Kuhn, P., Earnest, T. and Stevens, R.C. (2000) Nat. Struct. Biol Suppl, 973–977Google Scholar
  6. 6.
    Strausberg, R.L., Feingold, E.A., Klausner, R.D., Collins, F.S. 1999Science286455457CrossRefPubMedGoogle Scholar
  7. 7.
    MGC (Mammalian Gene Collection) Program Team2002Proc. Natl. Acad. Sci. U.S.A.991689916903Google Scholar
  8. 8.
    Liu, Q., Li, M.Z., Leibham, D., Cortez, D., Elledge, S.J. 1999Curr. Biol.813001309CrossRefGoogle Scholar
  9. 9.
    Hatley, J.L., Temple, G.F., Brasch, M.A. 2000Genome Res.1017881795CrossRefPubMedGoogle Scholar
  10. 10.
    Johnston, S., Lee, J.H., Ray, D.S. 1985Gene3413745CrossRefPubMedGoogle Scholar
  11. 11.
    Page, R., Grzechnik, S.K., Kreusch, A., Spraggon, G., Stevens, R.C., Lesley, S.A. 2003Acta Cryst. D.5910281037CrossRefGoogle Scholar
  12. 12.
    Wootton, J.C., Federhen, S. 1993Comput. Chem.17149163CrossRefGoogle Scholar
  13. 13.
    Linding, R., Russell, R.B., Neduva, V., Gibson, T.J. 2003Nucleic Acids Res.3137013708CrossRefPubMedGoogle Scholar
  14. 14.
    Pantazatos, D., Kim, J.S., Klock, H.E., Stevens, R.C., Wilson, I.A., Lesley, S.A., Woods, V.L. 2004Proc. Natl. Acad. Sci. U.S.A.101751756CrossRefPubMedGoogle Scholar
  15. 15.
    Spraggan, G., Pantazatos, D., Klock, H.E., Wilson, I.A., Woods, V.L.,Jr., Lesley, S.A. 2004Protein Sci.1331873199CrossRefPubMedGoogle Scholar
  16. 16.
    Goh, C.S., Lan, N., Douglas, S.M., Wu, B., Echols, N., Smith, A., Milburn, D., Montelione, G.T., Zhao, H., Gerstein, M. 2004J. Mol. Biol.336115130CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Joint Center for Structural GenomicsGenomics Institute of the Novartis Research FoundationSan DiegoUSA
  2. 2.Joint Center for Structural GenomicsThe Scripps Research InstituteLa JollaUSA

Personalised recommendations