Protein Production and Crystallization at the Joint Center for Structural Genomics

Article

Abstract

By definition, structural genomics centers must be able to address a large number of diverse protein targets. The methods developed should permit parallel and cost-effective processing while allowing for the diverse nature of proteins. Our approach to this problem is a multi-tiered effort where targets are characterized and categorized by behavior and processed in parallel by appropriate methods. The Joint Center for Structural Genomics (JCSG) has applied this tactic to create a fully integrated and scaleable structure determination pipeline. Highlights of the development of the current pipeline for protein production and crystallization are presented here.

Key words

high throughput automated protein production robotic crystallization salvage pathways protein characterization 

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Joint Center for Structural GenomicsGenomics Institute of the Novartis Research FoundationSan DiegoUSA
  2. 2.Joint Center for Structural GenomicsThe Scripps Research InstituteLa JollaUSA

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