Journal of Structural and Functional Genomics

, Volume 6, Issue 2–3, pp 177–182 | Cite as

SOn-column Protein Refolding for Crystallization

Article

Abstract

One major bottleneck in protein production in Escherichia coli for structural genomics projects is the formation of insoluble protein aggregates (inclusion bodies). The efficient refolding of proteins from inclusion bodies is becoming an important tool that can provide soluble native proteins for structural and functional studies. Here we report an on-column refolding method established at the Berkeley Structural Genomics Center (BSGC). Our method is a combination of an ‘artificial chaperone-assisted refolding’ method previously proposed and affinity chromatography to take advantage of a chromatographic step: less time-consuming, no filtration or concentration, with the additional benefit of protein purification. It can be easily automated and formatted for high-throughput process.

Key words

crystallization recombinant proteins refolding 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Natalia Oganesyan
    • 1
  • Sung-Hou Kim
    • 1
    • 2
  • Rosalind Kim
    • 1
  1. 1.Berkeley Structural Genomics Center, Physical Biosciences DivisionLawrence Berkeley National LaboratoryBerkeleyUSA
  2. 2.Department of ChemistryUniversity of CaliforniaBerkeleyUSA

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