Synthesis of high molar activity 33P-labeled phosphorous acid
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Studies of phosphorus cycling in the ocean have been greatly facilitated by the use of high molar activity 32P- and 33P-labeled phosphate (phosphoric acid) in biological incubation assays. Recently, phosphite (phosphorous acid) has been shown to play an important role in the ocean. Here I report the microscale (100 μmol) synthesis of high molar activity 33P-labeled phosphorous acid. The scheme incorporates a new combination of known synthetic routes, which requires 20 times less radioactivity than existing methods. The economical production of 33P-phosphorous acid with molar activity > 37 GBq mol−1 for use in assays is readily achievable with this scheme.
KeywordsPhosphorus Phosphorous acid Phosphite 33P 32P Synthesis
I gratefully acknowledge Carl Johnson (Woods Hole Oceanographic Institution) for conducting the 31P NMR analyses. This work was supported by a grant from the Simons Foundation (329108 to B.A.S.V.M.), and is a contribution of SCOPE. This work was also supported by the National Science Foundation (OCE-1536346 to B.A.S.V.M.).
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