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Vibrational spectroscopy as a tool for examination to the secondary structure of metal-labeled trastuzumab immunoconjugates

  • M. Sterjova
  • P. Džodić
  • P. Makreski
  • A. Duatti
  • M. Risteski
  • E. Janevik-IvanovskaEmail author
Article

Abstract

Infrared and Raman spectroscopy are effective techniques that allow collecting information about secondary structure of proteins, including antibodies. Trastuzumab, antibody used in our study was in a freeze-dried form, conjugated with different bifunctional chelators and linked with the stable isotopes of lutetium and yttrium. The characterization of the final immunoconjugates showed no significant changes in the structure demonstrated by the presence of the amide bands characteristic for a α-helices and β-sheets structures. These methods could be applied during the production of the antibody freeze-dried kit formulations for the labeling with the radioactive isotopes.

Keywords

Trastuzumab Freeze-drying Infrared spectroscopy Raman spectroscopy Bifunctional chelators Yttrium and lutetium 

Notes

Acknowledgements

This research was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 172044) and by the University Goce Delcev, Faculty of Medical Sciences, Stip, Republic of Macedonia (Grant No. 0201-165/6). Commercial Herceptin® was provided by University Clinic for Radiotherapy and Oncology, Skopje. The authors thank R&D Institute, Alkaloid AD, Skopje for making available the ATR-IR instrumentation to collect the corresponding spectra.

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  1. 1.Faculty of Medical SciencesGoce Delčev UniversityŠtipRepublic of Macedonia
  2. 2.Department of Pharmacy, Faculty of MedicineUniversity of NišNišSerbia
  3. 3.Faculty of Natural Sciences and Mathematics, Institute of ChemistrySs. Cyril and Methodius UniversitySkopjeRepublic of Macedonia
  4. 4.Department of Chemical and Pharmaceutical SciencesUniversity of FerraraFerraraItaly
  5. 5.University Clinic of Radiotherapy and OncologySkopjeRepublic of Macedonia

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