Advertisement

Radiolabeling of DNA nanostructure with 99mTc using DTPA as a chelate

  • Xiaoyan Duan
  • Jianbo Li
  • Xiaoling Lu
Article
  • 10 Downloads

Abstract

In the field of nanotechnology, DNA-based nanoscale materials have facilitated the construction of DNA polyhedrons with different shapes and sizes by using predictable base pairing and highly tunable conformation. In this study DNA bipyramid nanostructures with one arm chain (T20-DBNs) were successfully prepared in a single annealing procedure. 99mTc-A20 ssDNA was obtained by radiolabeled DTPA-A20 with 99mTc, and then 99mTc-DTPA-DBNs were obtained by hybridizing T20-DBNs with 99mTc-A20 ssDNA. We focused on studying a method of 99mTc radiolabeling DNA nanostructures with DTPA as a chelate, and hoped to develop a new SPECT molecular imaging probe based on DNA nanostructures.

Keywords

Radiolabeling 99mTc DNA bipyramid nanostructures Molecular imaging probes 

Notes

Acknowledgements

The authors’ work was supported by National Natural Science Foundation of China (No. 81360227) and Natural Science Foundation of Inner Mongolia (2018LH08044).

References

  1. 1.
    Travers A, Muskhelishvili G (2015) DNA structure and function. FEBS J 282(12):2279–2295CrossRefGoogle Scholar
  2. 2.
    Ke Y, Castro C, Choi JH (2018) Structural DNA nanotechnology: artificial nanostructures for biomedical research. Annu Rev Biomed Eng. doi:10.1146/annurev-bioeng-062117-120904CrossRefGoogle Scholar
  3. 3.
    Goodman RP, Schaap IAT, Tardin CF, Erben CM, Berry RM, Schmidt CF, Turberfield AJ (2005) Rapid chiral assembly of rigid DNA building blocks for molecular nanofabrication. Science 310(5754):1661CrossRefGoogle Scholar
  4. 4.
    Erben CM, Goodman RP, Turberfield AJ (2007) A self-assembled DNA bipyramid. J Am Chem Soc 129(22):6992–6993CrossRefGoogle Scholar
  5. 5.
    Zhang C, Ko SH, Su M, Leng Y, Ribbe AE, Jiang W, Mao C (2009) Symmetry controls the face geometry of DNA polyhedra. J Am Chem Soc 131(4):1413–1415CrossRefGoogle Scholar
  6. 6.
    Jiang D, England CG, Cai W (2016) DNA nanomaterials for preclinical imaging and drug delivery. J Control Release 239(2016):27–38CrossRefGoogle Scholar
  7. 7.
    Moghimi SM, Hunter AC, Murray JC (2005) Nanomedicine: current status and future prospects. FASEB J 19(3):311–330CrossRefGoogle Scholar
  8. 8.
    Kobayashi H, Watanabe R, Choyke PL (2014) Improving conventional enhanced permeability and retention (EPR) effects; what is the appropriate target? Theranostics 4(1):81–89CrossRefGoogle Scholar
  9. 9.
    Li J, Jiang D, Bao B, He Y, Liu L, Wang X (2016) Radiolabeling of DNA bipyramid and preliminary biological evaluation in mice. Bioconjugate Chem 27(4):905–910CrossRefGoogle Scholar
  10. 10.
    Keum J-W, Bermudez H (2009) Enhanced resistance of DNA nanostructures to enzymatic digestion. Chem Commun 45:7036–7038CrossRefGoogle Scholar
  11. 11.
    Perrault SD, Shih WM (2014) Virus-inspired membrane encapsulation of DNA nanostructures to achieve in vivo stability. ACS Nano 8(5):5132–5140CrossRefGoogle Scholar
  12. 12.
    Jiang D, Sun Y, Li J, Li Q, Lv M, Zhu B, Tian T, Cheng D, Xia J, Zhang L, Wang L, Huang Q, Shi J, Fan C (2016) Multiple-armed tetrahedral DNA nanostructures for tumor-targeting, dual-modality in vivo imaging. ACS Appl Mater Inter 8(7):4378–4384CrossRefGoogle Scholar
  13. 13.
    Lee Hyukjin, Lytton-Jean Abigail K R, Chen Yi, Love Kevin T, Park Angela I, Karagiannis Emmanouil D, Sehgal Alfica, Querbes William, Zurenko Christopher S, Jayaraman Muthusamy, Peng Chang G, Charisse Klaus, Borodovsky Anna, Manoharan Muthiah, Donahoe Jessica S, Truelove Jessica, Nahrendorf Matthias, Langer Robert, Anderson Daniel G (2012) Molecularly self-assembled nucleic acid nanoparticles for targeted in vivo siRNA delivery. Nat Nanotechnol 7(6):389–393CrossRefGoogle Scholar
  14. 14.
    Li J, Wang X, Bao B, He Y, Wang X, Zhang G, Bai X, Liu L (2016) Radiolabeling of DNA bipyramid nanostructures. Chem J Chin U 37(9):1655–1659Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2018

Authors and Affiliations

  1. 1.Inner Mongolia Medical University, Affiliated HospitalHohhotChina

Personalised recommendations