Journal of Radioanalytical and Nuclear Chemistry

, Volume 317, Issue 2, pp 1133–1139 | Cite as

Stability assessment of a bovine kidney reference-material candidate

  • Liliana Castro
  • Edson G. Moreira
  • Marina B. A. Vasconcellos
  • Camila N. Lange
  • Tatiana Pedron
  • Bruno L. Batista


Reference materials are used worldwide and necessary for quality control purposes during analytical determinations. The present study describes the stability evaluation of a bovine kidney reference-material candidate. An isochronous layout was performed, in which the flasks involved are exposed at different temperatures for different time periods and then are analyzed at the same time at the end of the study. The mass fractions of ten inorganic constituents were determined using instrumental neutron activation analysis and inductively coupled plasma mass spectrometry. Statistical analysis, univariate and multivariate, showed no significant differences in composition between units exposed to the different temperatures and times. The reference material may be transported under normal transportation conditions and the certified values and uncertainties will continue to be valid for a period of 2 years.


Bovine kidney reference material Stability study Neutron activation analysis Inductively coupled plasma mass spectrometry Principal component analysis 



The authors would like to acknowledge the financial support of CNPq (Brazilian National Council for Scientific and Technological Development), from Research fellowship 307093/2013-1. The author L. Castro would like also to acknowledge the Ph. D. fellowship from CAPES (Coordination for the Improvement of Higher Education Personnel).


  1. 1.
    FAO (Food and Agriculture Organization of the United Nations) The state of food and agriculture. Food systems for better nutrition, 2013. Rome. Accessed 20 July 2017
  2. 2.
    FAO (Food and Agriculture Organization of the United Nations). Food balance.*/E. Accessed 20 March 2017
  3. 3.
    Castro L, Moreira EG, Vasconcellos MBA (2017) Use of INAA in the homogeneity evaluation of a bovine kidney candidate reference material. J Radioanal Nucl Chem 311:1291–1298CrossRefGoogle Scholar
  4. 4.
    Lamberty A, Schimmel H, Pauwels J (1998) The study of the stability of reference materials by isochronous measurements. Fresenius J Anal Chem 360:359–361CrossRefGoogle Scholar
  5. 5.
    Linsinger TPJ, Pauwels J, Van der Veen AMH, Schimmel H, Lamberty A (2001) Homogeneity and stability of reference materials. Accred Qual Assur 6:20–25CrossRefGoogle Scholar
  6. 6.
    Van der Veen AMH, Linsinger TPJ, Lamberty A, Pauwels J (2001) Uncertainty calculations in the certification of reference materials. 3. Stability study. Accred Qual Assur 6:257–263CrossRefGoogle Scholar
  7. 7.
    Pauwels J, Lamberty A, Schimmel H (1998) Quantification of the expected shelf-life of certified reference. Fresenius J Anal Chem 361:395–399CrossRefGoogle Scholar
  8. 8.
    Linsinger TPJ, Van der Veen AMH, Gawlik BM, Pauwels J, Lamberty A (2004) Planning and combining of isochronous stability studies of CRMs. Accred Qual Assur 9:464–472CrossRefGoogle Scholar
  9. 9.
    Jolliffe IT (2002) Principal component analysis, 2nd edn. Springer, New YorkGoogle Scholar
  10. 10.
    Wold S, Esbensen K, Geladi P (1987) Principal component analysis. Chemometr Intell Lab Syst 2:37–52CrossRefGoogle Scholar
  11. 11.
    Lima DC, Santos AMP, Araujo RGO, Scarminio IS, Bruns RE, Ferreira SLC (2010) Principal component analysis and hierarchical cluster analysis for homogeneity evaluation during the preparation of a wheat flour laboratory reference material for inorganic analysis. Microchem J 95:222–226CrossRefGoogle Scholar
  12. 12.
    Santos AMP, Santos LO, Brandao GC, Leao DJ, Bernedo AVB, Lopes RT, Lemos VA (2015) Homogeneity study of a corn flour laboratory reference material candidate for inorganic analysis. Food Chem 178:287–291CrossRefPubMedGoogle Scholar
  13. 13.
    Carvalho Rocha WF, Nogueira R (2011) Use of multivariate statistical analysis to evaluate experimental results for certification of two pharmaceutical reference materials. Accred Qual Assur 16:523–528CrossRefGoogle Scholar
  14. 14.
    Carvalho Rocha WF, Nogueira R, da Silva GEB, Queiroz SM, Sarmanho GF (2013) A comparison of three procedures for robust PCA of experimental results of the homogeneity test of a new sodium diclofenac candidate certified reference material. Microchem J 109:112–116CrossRefGoogle Scholar
  15. 15.
    Sarembaud J, Pinto R, Rutledge DN, Feinberg M (2007) Application of the ANOVA-PCA method to stability studies of reference materials. Anal Chim Acta 603:147–154CrossRefPubMedGoogle Scholar
  16. 16.
    ISO (International Organization of Standardization) (2006) Certification of reference materials. General and statistical principles. 3rd edn. ISO Guide 35. ISO, GenevaGoogle Scholar
  17. 17.
    Linsinger TPJ, Pauwels J, Lamberty A, Schimmel H, Van der Veen AMH, Siekmann L (2001) Estimating the uncertainty of stability for matrix CRMs. Fresenius J Anal Chem 370:183–188CrossRefPubMedGoogle Scholar
  18. 18.
    Statsoft, Inc. (2005) STATISTICA 7.0 software, Tulsa, Oklahoma, USAGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2018

Authors and Affiliations

  1. 1.Instituto de Pesquisas Energéticas e Nucleares – IPEN - CNEN/SPSão PauloBrazil
  2. 2.Centro de Ciências Naturais e HumanasUniversidade Federal do ABCSanto AndréBrazil

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