15N-labelled pyrazines of triterpenic acids

  • Martin Vlk
  • Petra Micolova
  • Milan Urban
  • Miroslav Kvasnica
  • David Saman
  • Jan SarekEmail author


Triterpenoid pyrazines from our research group were found selectively cytotoxic on several cancer cell lines with IC50 in low micromolar range. This sparked our interest in preparing their labeled analogs for metabolic studies. In this work, we prepared a set of non-labeled pyrazines from seven triterpenoid skeletal types along with their 15N labelled analogs. In this work, we present the synthesis and characterization of the target 15N labelled pyrazines. Currently, these compounds are being studied in complex metabolic studies.


15Triterpenic acid Isotopic labelling Pyrazine Heterobetulinic acid 



This work was supported partially by the Ministry of Education, Youth and Sports—project LK21310, Technological Agency of the Czech Republic—project TA03010027, and CTU in Prague—project SGS15/094/OHK4/1T/14; the Czech Science Foundation, projects GJ15-08202Y and GA15-05620S and by NPUII projects LO1304 and LO1204. Special thanks to The Czech Institute of Informatics, Robotics and Cybernetics, CTU in Prague for HR-MS instrumentation. We are also grateful to Bohunka Šperlichová for measurement of optical rotatory power and Šárka Hráčková for measurement of melting points.


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2015

Authors and Affiliations

  • Martin Vlk
    • 1
  • Petra Micolova
    • 1
  • Milan Urban
    • 2
    • 3
  • Miroslav Kvasnica
    • 4
  • David Saman
    • 5
  • Jan Sarek
    • 2
    Email author
  1. 1.Faculty of Nuclear Sciences and Physical EngineeringCzech Technical University in PraguePrague 1Czech Republic
  2. 2.Faculty of Medicine and Dentistry, Institute of Molecular and Translational MedicinePalacky University OlomoucOlomoucCzech Republic
  3. 3.Department of Organic Chemistry, Faculty of SciencePalacky University OlomoucOlomoucCzech Republic
  4. 4.Laboratory of Growth Regulators, Centre of the Region Hana for Biotechnological and Agricultural ResearchInstitute of Experimental Botany ASCR & Palacky UniversityOlomoucCzech Republic
  5. 5.Institute of Organic Chemistry and BiochemistryASCRPrague 6Czech Republic

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