PET imaging of sterile inflammation with a 18F-labeled bis(zinc(II)-dipicolylamine) complex
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Small-molecular probe 18F-labeled bis(zinc(II)-dipicolylamine) complex (18F-FB-DPAZn2) was evaluated for PET imaging of sterile inflammation. In comparison with 18F-2-deoxy-β-d-glucose (18F-FDG), 18F-radiolabeled Annexin V (18F-FB-Annexin V) showed rapid clearance of radioactivity from the kidney and low uptake in most tissues. Both the lower radioactivity accumulation in brain and heart and the higher uptakes in the lung, liver, and intestine were observed for the biodistribution of 18F-FB-DPAZn2. In PET imaging, 18F-FDG showed significantly higher tumor and inflammation uptake than did of 18F-FB-DPAZn2 and 18F-FB-Annexin V in the S-180 fibrosarcoma mouse model and sterile inflammation mouse model. Both 18F-FB-DPAZn2 and 18F-FB-Annexin V performed the specifically localization in inflammation, and the ratios of inflammatory lesion-to-muscle and tumor-to-muscle were 1.83 ± 0.20 and 0.90 ± 0.12 (P < 0.05) for 18F-FB-DPAZn2, and 1.51 ± 0.14 and 1.21 ± 0.12 (P > 0.05) for 18F-FB-Annexin V, respectively. Terminal deoxynucleotide end-labeling (TUNEL) assays performed on the dissected tissues showed the significantly more TUNEL-positive nuclei in the inflammatory muscle than in tumor and normal muscle, and these TUNEL results correlated with the uptake of 18F-FB-DPAZn2 in dissected tissues. Biodistribution and PET imaging studies showed that the 18F-FB-DPAZn2 is suitable for imaging sterile inflammation in vivo and is capable of the differentiating tumor from inflammation.
KeywordsPositron emission tomography Sterile inflammation Phosphatidylserine Zinc(II)-dipicolylamine Cell death
The authors would like to thank Dr. Xiang song Zhang for his useful assistance. This work was supported by the National Natural Science Foundation (No. 81101076, 30970856, 81171374), Postdoctoral Science Foundation of China (20110490964), Science and Technology Planning Project of Guangdong Province, China (2010B031600054 and 2012B031800082) and Hundred Talents Program of Sun Yat-Sen University (No. 18901205).
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