Journal of Radioanalytical and Nuclear Chemistry

, Volume 295, Issue 3, pp 2043–2048 | Cite as

Determination of 129I in biomonitors collected in the vicinity of a nuclear power plant by neutron activation analysis

  • Ivana Krausová
  • Jan Kučera
  • Ivo Světlík


Neutron activation analysis (NAA) was used to determine 129I and the 129I/127I ratio in bovine thyroid, moss, and river sediment samples collected in the vicinity of the Temelín nuclear power plant (NPP) in south Bohemia. The NAA procedures comprised pre-irradiation separation of 129I by combustion of the samples in the stream of oxygen at 1,000 °C and trapping the liberated iodine in a LiOH/(NH4)2SO3 solution. Post-irradiation separation of 130I produced by the reaction 129I(n,γ)130I was carried out by extraction of elementary iodine with chloroform followed by precipitation of PdI2. Nondestructive, epithermal NAA was used to determine 127I employing the 127I(n,γ)128I reaction. The results showed that mean values of 129I and the 129I/127I ratio in the bovine thyroids varied from 22 to 61 mBq kg−1 (dry mass) and 2.8 × 10−9 to 5.4 × 10−9, respectively. These values are close to the lower end of results reported from various regions non-polluted with 129I. No significant differences were found between 129I concentrations and the 129I/127I ratios in the bovine thyroids collected prior to the start and after several years of operation of the NPP. The mean value and standard deviation of 129I in mBq kg−1, dry mass and the 129I/127I ratio in moss Pleurozium schreberi were 23 ± 16 and 2.3 × 10−9, respectively, whereas values of 129I in the river sediments were below 8–10 mBq kg−1 (dry mass) after several years of the NPP operation.


129Biomonitor Thyroid gland Neutron activation analysis Nuclear power plant 



Support from the Czech Science Foundation (grant P108/12/G108) for this work is appreciated.


  1. 1.
    Hou X, Hansen V, Aldahan A, Possnert G, Lind OC, Lujaniene G (2009) A review on speciation of iodine-129 in the environmental and biological samples. Anal Chim Acta 632:181–196CrossRefGoogle Scholar
  2. 2.
    Hou XL, Dahlgaard H, Nielsen SP, Kučera J (2002) Level and origin of iodine-129 in the Baltic Sea. J Environ Radioact 61:331–343CrossRefGoogle Scholar
  3. 3.
    Rao U, Fehn U (1999) Source and reservoirs of anthropogenic iodine-129 in western New York. Geochim Cosmochim Acta 63:1927–1938CrossRefGoogle Scholar
  4. 4.
    He C, Hou X, Zhao Y, Wang Z, Li H, Chen N, Liu Q, Zhang L, Luo M, Liang W, Fan Y, Zhao X (2011) 129I level in seawater near a nuclear power plant determined by accelerator mass spectrometer. Nucl Instrum Methods A 632:152–156CrossRefGoogle Scholar
  5. 5.
    Kučera J, Iyengar GV, Řanda Z, Parr RM (2004) Determination of iodine in Asian diets by epithermal and radiochemical neutron activation analysis. J Radioanal Nucl Chem 259:505–509CrossRefGoogle Scholar
  6. 6.
    Aumann DC, Buheitel F, Hauschild J, Robens E, Wershofen H (1987) Chemical and nuclear interferences in neutron activation of 129I and 127I in environmental samples. J Radioanal Nucl Chem 109:261–274CrossRefGoogle Scholar
  7. 7.
    Muramatsu Y, Uchida S, Sumiya M, Ohmomo Y (1985) Iodine separation procedure for the determination of 129I and 127I in soil by neutron activation analysis. J Radioanal Nucl Chem 94:329–338CrossRefGoogle Scholar
  8. 8.
    Katagari H, Narito O, Yamato A, Kinoshita M (1990) Low level measurement of 129I in environmental samples. J Radioanal Nucl Chem 138:187–192CrossRefGoogle Scholar
  9. 9.
    US National Institute of Standards & Technology (2006) Standard reference material 4949C iodine-129 radioactivity standardGoogle Scholar
  10. 10.
    Fréchou C, Calmet D, Bouisset P, Piccot D, Gaudry A, Yiou F, Raisbeck G (2001) 129I and 129I/127I ratio determination in environmental biological samples by RNAA, AMS and direct γ-X spectrometry measurement. J Radioanal Nucl Chem 249:133–138CrossRefGoogle Scholar
  11. 11.
    Van Middlesworth L (1993) 129I and 137Cs fission products in thyroids of animals. Health Phys 64:52–58CrossRefGoogle Scholar
  12. 12.
    Fréchou C, Calmet D, Bertho X, Gaudry A (2002) 129I/127I ratio measurements in bovine thyroids from the North Cotentin (France). Sci Total Environ 293:59–67CrossRefGoogle Scholar
  13. 13.
    Fréchou C, Calmet D (2003) 129I in the environment of the La Hague nuclear fuel reprocessing plant—from sea to land. J Environ Radioact 70:43–59CrossRefGoogle Scholar
  14. 14.
    Schmidt A, Schnabel Ch, Handl J, Jakob D, Michel R, Synal HA, Lopez JM, Sutter M (1998) On the analysis of iodine-129 and iodine-127 in environmental materials by accelerator mass spectrometry and ion chromatography. Sci Total Environ 223:131–156CrossRefGoogle Scholar
  15. 15.
    Oliver LL, Ballad RV, Manuel OK (1982) Iodine-129 in Missouri thyroids. Health Phys 42:425–432CrossRefGoogle Scholar
  16. 16.
    Handl J (1996) Concentrations of 129I in the biosphere. Radiochim Acta 72:33–38Google Scholar
  17. 17.
    Aumann DC, Faleschini H, Friedman L (1981) Determination of 129I and 127I at natural levels by neutron activation analysis. Radiochim Acta 29:209–215Google Scholar
  18. 18.
    Seki R, Hatano T (1994) Isotopic ratios of 129I/127I in mammalian thyroid glands in Japan. J Radiaoanal Nucl Chem 182:157–163CrossRefGoogle Scholar
  19. 19.
    Hou X, Dahlgaard H, Nielsen SP, Ding W (2000) Iodine-129 in human thyroids and seaweed in China. Sci Total Environ 246:285–291CrossRefGoogle Scholar
  20. 20.
    Chao JH, Tseng CL (1996) I-129 concentration of mammalian thyroids in Taiwan. Sci Total Environ 193:111–119CrossRefGoogle Scholar
  21. 21.
    Iyengar GV, Kolmer WE, Bowen HJM (1978) Elemental composition of human tissues and body fluids. Verlag Chemie, WeinheimGoogle Scholar
  22. 22.
    Fehn U, Snyder G (2000) 129I in the southern hemisphere: global redistribution of an anthropogenic isotope. Nucl Instrum Methods B 172:366–371CrossRefGoogle Scholar
  23. 23.
    Handl J, Oliver E, Jakob D, Johanson KJ, Schuller P (1993) Biospheric 129I concentrations in the pre-nuclear and nuclear age. Health Phys 65:265–271CrossRefGoogle Scholar
  24. 24.
    Daillant OR, Bernollin A, Josset M, Fifield KL (2009) Potential of lichens for monitoring iodine-129 and chlorine-36. J Radioanal Nucl Chem 281:241–245Google Scholar
  25. 25.
    Handl J, Pfau A, Huth FW (1990) Measurements of 129I in human and bovine thyroids in Europe—transfer of 129I into the food chain. Health Phys 58:609–618CrossRefGoogle Scholar
  26. 26.
    Doshi GR, Joshi SN, Pillai KC (1991) 129I in soil and grass samples around a nuclear reprocessing plant. J Radioanal Nucl Chem 155:115–127CrossRefGoogle Scholar
  27. 27.
    Muramatsu Y, Yoshida S (1995) Determination of 129I and 127I in environmental samples by neutron activation analysis (NAA) and inductively coupled plasma mass spectrometry (ICP-MS). J Radioanal Nucl Chem 197:149–159CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2012

Authors and Affiliations

  1. 1.Nuclear Physics Institute ASCRŘež 130Czech Republic

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