Journal of Radioanalytical and Nuclear Chemistry

, Volume 287, Issue 1, pp 119–127 | Cite as

Preparation and biological evaluation of radiolabeled-folate embedded superparamagnetic nanoparticles in wild-type rats

  • Amir Reza JalilianEmail author
  • Seyyedeh Leila Hosseini-Salekdeh
  • Morteza Mahmoudi
  • Hassan Yousefnia
  • A. Majdabadi
  • Majid Pouladian


In this study, superparamagnetic iron oxide nanoparticles (SPION) embedded by folic acid (SPION-folate) were prepared by a modified co-precipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-folate were characterized systematically by means of XRD, VSM, HRSEM and TEM and the interaction between folate and iron oxide (Fe3O4) was characterized by FT-IR. The particle size was shown to be ≈5–10 nm. To ensure biocompatibility, the interaction of these SPION with mouse connective tissue cells (adhesive) was investigated using an MTT assay. Consequently, gallium-67 labeled nanoparticles ([67Ga]-SPION-folate) were prepared using 67Ga with a high labeling efficiency (over 96%, RTLC method) and they also showed an excellent stability at room temperature for at least 2 days and were evaluated for their biodistribution in normal rats up to 24 h compared with free Ga3+ cation and [67Ga]-SPION biodistribution. The biodistribution of the tracer among 3 other folate tracers were compared, showing lower liver uptake and higher blood circulation after 24 h leading to better bioavailability. The bone:muscle, kidney:muscle, lung:muscle, stomach:muscle ratios were 9.3, 9.32, 7.6 and 5.83 respectively. The developed folate-containing nano-system can be an interesting folate receptor tracer, capable of better cell membrane permeability while possessing paramagnetic properties for thermotherapy.


SPION 67Ga Folate Biodistribution Quality control 



Authors wish to thank Mr S. Daneshvari for conducting animal studies and radioisotope production team at Nuclear Medicine Research Group and Dr. M. A. Rowshanzamir for editorial corrections.


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2010

Authors and Affiliations

  • Amir Reza Jalilian
    • 1
    Email author
  • Seyyedeh Leila Hosseini-Salekdeh
    • 2
  • Morteza Mahmoudi
    • 3
  • Hassan Yousefnia
    • 1
  • A. Majdabadi
    • 1
  • Majid Pouladian
    • 2
  1. 1.Radiopharmaceutical Research and Development Lab (RRDL)Nuclear Science and Technology Research Institute (NSTRI)TehranIran
  2. 2.Department of Engineering and Technical, Science and Research BranchIslamic Azad UniversityTehranIran
  3. 3.Institute for Nanoscience and NanotechnologySharif University of TechnologyTehranIran

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