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Real-time imaging of ion uptake from root to above-ground part of the plant using conventional beta-ray emitters

  • T. M. Nakanishi
  • M. Yamawaki
  • S. Kannno
  • N. Nihei
  • S. Masuda
  • K. Tanoi
Article

Abstract

We present real-time imaging of 32P-phosphate within a living plant as well as that in culture solution toward roots. The above-ground part of the plant was irradiated with light from LED and roots were kept in dark. The plant was Lotus japonicus, cv. Miyakojima MG20 and real-time 32P-phosphate uptake manners in young, flowering and adult phase were visualized. In the case of young plant, there was a preferential translocation of 32P in younger leaves. When the sample was treated with phosphate deficient condition, 32P uptake amount was much higher. In an adult phase, 32P uptake was high at pods.

Keywords

Real-time imaging Lotus japonicus 32P-phosphate uptake 32P-phosphate accumulation Image analysis 

References

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2009

Authors and Affiliations

  • T. M. Nakanishi
    • 1
  • M. Yamawaki
    • 1
  • S. Kannno
    • 1
  • N. Nihei
    • 1
  • S. Masuda
    • 1
  • K. Tanoi
    • 1
  1. 1.Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan

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