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Optimization studies for encapsulation and controlled release of curcumin drug using Zn+2 cross-linked alginate and carboxy methylcellulose blend

  • Ching-Hwa Lee
  • Lakshmi P. NalluriEmail author
  • Srinivasa R. Popuri
ORIGINAL PAPER
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Abstract

Biopolymers have attracted significant attention for the development of drug-carrying systems due to their high degree of biocompatibility, nontoxicity, biodegradability, and renewability. The focus of this work is to examine the optimized conditions for maximum encapsulation and release of anti-cancer drug curcumin using modified biopolymer blend beads of sodium alginate (SA) and carboxymethylcellulose (CMC). Biopolymer blend beads were prepared by mixing equal amounts of SA and CMC using normal syringe method followed by crosslinking with ZnCl2 to improve the stability of the drug carrier. The applicability of the Zn2+ Crosslinked SA/CMC for curcumin was evaluated in terms of entrapment efficiency, swelling, in vitro release and drug release kinetics. Further, pseudo-first order and pseudo-second-order kinetics and isotherm models were applied to the encapsulation data and the results demonstrate that the interaction between curcumin and the biopolymers was physio sorption. The developed biopolymer drug carriers were characterized by Fourier transform infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and X-ray Diffraction (XRD) with and without loading of curcumin to investigate the interactions between the curcumin drug and drug carrier. The obtained results were also analyzed and compared with pristine SA beads. The maximum encapsulation efficiency was found to be 79.75% and 93.07% for SA and SA/CMC blend respectively for the formulations. The in vitro drug release studies were carried out at pH 7.4 in phosphate buffer for 8 h at 37 °C and the results demonstrate the cumulative percentage of curcumin release was increased with increasing carrier concentration. Among several kinetic models of drug release, Korsmeyer-Peppas model exhibited high correlation coefficient (r2) for both the SA and the SA/CMC.

Keywords

Sodium alginate Carboxymethylcellulose Curcumin Drug release Korsmeyer-Peppas equation 

References

  1. 1.
    Eswaramma S, Sivagangi Reddy N, Krishna Rao KSV (2017) Carbohydrate polymer based pH-sensitive IPN microgels synthesis, characterization and drug release characteristics. Mater Chem Phys 195:176–186CrossRefGoogle Scholar
  2. 2.
    Akindele AJ, Wani ZA, Sharma S, Mahajan G, Satti NK, Adeyemi O, Saxena AK (2015) In vitro and in vivo anticancer activity of root extracts of Sansevieria liberica Gerome and Labroy (Agavaceae). Evid Based Complement Alternat Med 560404:1–11Google Scholar
  3. 3.
    Ranquin A, Versees W, Meier W, Steyaert J, Van Gelder P (2005) Therapeutic nonreactors: combining chemistry and biology in a novel triblock copolymer drug delivery system. Nano Lett 5(11):2220–2224CrossRefGoogle Scholar
  4. 4.
    Chia S, Bryce C, Gelmon K (2005) Effects of chemotherapy and hormonal therapy for early breast cancer on recurrence and 15-year survival, an overview of the randomised trials. Lancet 365(9472):1687–1717CrossRefGoogle Scholar
  5. 5.
    Qin S, Seo JW, Zhang H, Qi J, Curry FRE, Ferrara KW (2010) An imaging-driven model for liposomal stability and circulation. Mol Pharm 7(1):12–21CrossRefGoogle Scholar
  6. 6.
    Upadhyaya L, Singh J, Agarwal V, Tewari RP (2014) The implications of recent advances in carboxymethyl chitosan based targeted drug delivery and tissue engineering applications. J Control Release 186:54–87CrossRefGoogle Scholar
  7. 7.
    Deng L, Kang X, Liu Y, Feng F, Zhang H (2017) Effects of surfactants on the formation of gelatin nanofibres for controlled release of curcumin. Food Chem 231(15):70–77CrossRefGoogle Scholar
  8. 8.
    Martins AF, Bueno PV, Almeida EA, Rodrigues FH, Rubira AF, Muniz EC (2013) Characterization of N-trimethyl chitosan/alginate complexes and curcumin release. Int J Biol Macromol 57:174–184CrossRefGoogle Scholar
  9. 9.
    Petchsomrit A, Sermkaew N, Wiwattanapatapee R (2017) Alginate-based composite sponges as gastroretentive carriers for curcumin-loaded self-micro emulsifying drug delivery systems. Sci Pharm 85(1):11CrossRefGoogle Scholar
  10. 10.
    Zheng Y, Monty J, Linhardt RJ (2015) Polysaccharide-based nanocomposites and their applications. Carbohydr Res 405:23–32CrossRefGoogle Scholar
  11. 11.
    Zhang Y, Bai Y, Chen H, Huang Y, Yuan P, Zhang L (2017) Preparation of a colon-specific sustained-release capsule with curcumin-loaded SMEDDS alginate beads. RSC Adv 7(36):22280–22285CrossRefGoogle Scholar
  12. 12.
    Madusanka N, de Silva KN, Amaratunga G (2015) A curcumin activated carboxymethyl cellulose–montmorillonite clay nanocomposite having enhanced curcumin release in aqueous media. Carbohydr Polym 134:695–699CrossRefGoogle Scholar
  13. 13.
    Grumezescu AM, Andronescu E, Ficai A, Bleotu C, Mihaiescu DE, Chifiriuc MC (2012) Synthesis, characterization and in vitro assessment of the magnetic chitosan–carboxymethylcellulose biocomposite interactions with the prokaryotic and eukaryotic cells. Int J Pharm 436:771–777CrossRefGoogle Scholar
  14. 14.
    Mohanty C, Sahoo SK (2017) Curcumin and its topical formulations for wound healing applications. Drug Discov Today 22:1582–1592CrossRefGoogle Scholar
  15. 15.
    Zheng B, Zhang Z, Chen F, Luo X, McClements DJ (2017) Impact of delivery system type on curcumin stability: comparison of curcumin degradation in aqueous solutions, emulsions, and hydrogel beads. Food Hydrocoll 71:187–197CrossRefGoogle Scholar
  16. 16.
    Nguyen ATB, Winckler P, Loison P, Wache Y, Chambin O (2014) Physico-chemical state influences in vitro release profile of curcumin from pectin beads. Colloids Surf B: Biointerfaces 121(1):290–298CrossRefGoogle Scholar
  17. 17.
    Gong C, Deng S, Wu Q, Xiang M, Wei X, Li L, Li Y (2013) Improving antiangiogenesis and anti-tumor activity of curcumin by biodegradable polymeric micelles. Biomaterials 34(4):1413–1432CrossRefGoogle Scholar
  18. 18.
    Lertsutthiwong P, Noomun K, Jongaroonngamsang N, Rojsitthisak P, Nimmannit U (2008) Preparation of alginate nanocapsules containing turmeric oil. Carbohydr Polym 74(2):209–214CrossRefGoogle Scholar
  19. 19.
    Esmaili M, Ghaffari SM, Moosavi-Movahedi Z, Atri MS, Sharifizadeh A, Farhadi M, Moosavi-Movahedi AA (2011) Beta casein-micelle as a nano vehicle for solubility enhancement of curcumin, food industry application. LWT-Food Sci Technol 44(10):2166–2172CrossRefGoogle Scholar
  20. 20.
    Huang X, Zheng X, Xu Z, Yi C (2017) ZnO-based nanocarriers for drug delivery application: from passive to smart strategies. Int J Pharm 534:190–194CrossRefGoogle Scholar
  21. 21.
    Zhang Z, Zhang R, Zou L, McClements DJ (2016) Protein encapsulation in alginate hydrogel beads: effect of pH on microgel stability, protein retention and protein release. Food Hydrocoll 58:308–315CrossRefGoogle Scholar
  22. 22.
    Verderio P, Bonetti P, Colombo M, Pandolfi L, Prosperi D (2013) Intracellular drug release from curcumin-loaded PLGA nanoparticles induces G2/M block in breast cancer cells. Biomacromolecules 14(3):672–682CrossRefGoogle Scholar
  23. 23.
    Al-Remawi M (2016) Quality by design of curcumin-loaded calcium alginate emulsion beads as an oral controlled release delivery system. J Exc Food Chem 6(2):930Google Scholar
  24. 24.
    El-Sherbiny IM, Smyth HD (2011) Controlled release pulmonary administration of curcumin using swellable biocompatible microparticles. Mol Pharm 9(2):269–280CrossRefGoogle Scholar
  25. 25.
    Bajpai SK, Kirar N (2016) Swelling and drug release behavior of calcium alginate/poly (sodium acrylate) hydrogel beads. Des Monomers Polym 19(1):89–98CrossRefGoogle Scholar
  26. 26.
    Song S, Wang Z, Qian Y, Zhang L, Luo E (2012) The release rate of curcumin from calcium alginate beads regulated by food emulsifiers. J Agric Food Chem 60(17):4388–4395CrossRefGoogle Scholar
  27. 27.
    Dhanaraju M, Sundar V, NandhaKumar S, Bhaskar K (2009) Development and evaluation of sustained delivery of diclofenac sodium from hydrophilic polymeric beads. J Young Pharm 1(4):301–304CrossRefGoogle Scholar
  28. 28.
    Sharma RK, Shaikh S, Ray D, Aswal VK (2018) Binary mixed micellar systems of PEO-PPO-PEO block copolymers for lamotrigine solubilization: a comparative study with hydrophobic and hydrophilic copolymer. J Polym Res 25(3):73CrossRefGoogle Scholar
  29. 29.
    Han Y, Wang L (2017) Sodium alginate/carboxymethyl cellulose films containing pyrogallic acid: physical and antibacterial properties. J Sci Food Agric 97(4):1295–1301CrossRefGoogle Scholar
  30. 30.
    Moussawi RN, Patra D (2016) Modification of nanostructured ZnO surfaces with curcumin: fluorescence-based sensing for arsenic and improving arsenic removal by ZnO. RSC Adv 6(21):17256–17268CrossRefGoogle Scholar
  31. 31.
    Wanninger S, Lorenz V, Subhan A, Edelmann FT (2015) Metal complexes of curcumin–synthetic strategies, structures and medicinal applications. Chem Soc Rev 44(15):4986–5002CrossRefGoogle Scholar
  32. 32.
    Thangaraju E, Rajiv S, Natarajan TS (2015) Comparison of preparation and characterization of water-bath collected porous poly L–lactide microfibers and cellulose/silk fibroin based poly L-lactide nanofibers for biomedical applications. J Polym Res 22(2):24CrossRefGoogle Scholar
  33. 33.
    Moussawi RN, Patra D (2016) Nanoparticle self-assembled grain like curcumin conjugated ZnO: curcumin conjugation enhances removal of perylene, fluoranthene, and chrysene by ZnO. Sci Rep 6:24565CrossRefGoogle Scholar
  34. 34.
    Costello LC, Franklin RB (2017) Decreased zinc in the development and progression of malignancy: an important common relationship and potential for prevention and treatmentofcarcinomas. Expert Opin Ther Targets 21(1):51–66CrossRefGoogle Scholar
  35. 35.
    Al-Ali K, Fatah HSA, El-Badry YAM (2016) Dual effect of curcumin–zinc complex in controlling diabetes mellitus in experimentally induced diabetic rats. Biol Pharm Bull 39(11):1774–1780CrossRefGoogle Scholar
  36. 36.
    Sun J, Liu J, Pan X, Quimby D, Zanesi N, Druck T, Huebner K (2010) Effect of zinc supplementation on N-nitrosomethylbenzylamine-induced forestomach tumor development and progression in tumor suppressor-deficient mouse strains. Carcinogenesis 32(3):351–358CrossRefGoogle Scholar
  37. 37.
    Prasad AS, Beck F, Snell W, DC Kucuk O (2009) Zinc in cancer prevention. Nutr Cancer 61(6):879–887CrossRefGoogle Scholar
  38. 38.
    Fong L, Jiang Y, Y Rawahneh ML, Smalley KJ, Croce CM, Farber JL, Huebner K (2011) Zinc supplementation suppresses 4-nitroquinoline 1-oxide-induced rat oral carcinogenesis. Carcinogenesis 32(4):554–560CrossRefGoogle Scholar
  39. 39.
    Jain N, Sareen R, Mahindroo N, Dhar KL (2014) Development and optimization of osmotically controlled asymmetric membrane capsules for delivery of solid dispersion of lycopene. Sci World J 438528:1–7Google Scholar
  40. 40.
    Das RK, Kasoju N, Bora U (2010) Encapsulation of curcumin in alginate-chitosan-pluronic composite nanoparticles for delivery to cancer cells. Nanomedicine 6(1):153–160CrossRefGoogle Scholar
  41. 41.
    Sarkar S, Mazumder S, Saha J, Bandyopadhyay S (2016) Management of inflammation by natural polyphenols: a comprehensive mechanistic update. Curr Med Chem 23(16):1657–1695CrossRefGoogle Scholar
  42. 42.
    Palmer D, Levina M, Nokhodchi A, Douroumis D, Farrell T, Rajabi-Siahboomi A (2011) The influence of sodium carboxymethylcellulose on drug release from polyethylene oxide extended release matrices. AAPS PharmSciTech 12(3):862–871CrossRefGoogle Scholar
  43. 43.
    Shaji J, Shaikh M (2016) Formulation, optimization, and characterization of biocompatible inhalable D-cycloserine-loaded alginate-chitosan nanoparticles for pulmonary drug delivery. Asian J Pharm Clin Res 9:82–95CrossRefGoogle Scholar
  44. 44.
    Riyajan SA, Nuim J (2013) Interaction of green polymer blend of modified sodium alginate and carboxylmethyl cellulose encapsulation of turmeric extract. Int J Polym Sci 2013:1–10CrossRefGoogle Scholar
  45. 45.
    Petchsomrit A, Sermkaew N, Wiwattanapatapee R (2013) Effect of alginate and surfactant on physical properties of oil entrapped alginate bead formulation of curcumin. World Academy of Science, Engineering and Technology, International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering 7(12):864–868Google Scholar
  46. 46.
    Lagergren SK (1898) About the theory of so-called adsorption of soluble substances. Sven Vetenskapsakad Handingarl 24:1–39Google Scholar
  47. 47.
    Ho YS, McKay G (1999) Pseudo-second order model for sorption processes. Process Biochem 34(5):451–465CrossRefGoogle Scholar
  48. 48.
    Malesu VK, Sahoo D, Nayak PL (2011) Chitosan–sodium alginate nanocomposites blended with cloisite 30b as a novel drug delivery system for anticancer drug curcuminGoogle Scholar
  49. 49.
    Mohanty DP, Palve YP, Sahoo D, Nayak P (2012) Synthesis and characterization of chitosan/cloisite 30B (MMT) nanocomposite for controlled release of anticancer drug curcumin. Int Pharma Res Alli Sci 1(4):52–62Google Scholar
  50. 50.
    Kumar Nagabandi V, Ramarao T, Jayaveera KN (2011) Liquisolid compacts: a novel approach to enhance bioavailability of poorly soluble drugs. Int J Pharm Res Allied Sci 1(3):89–102Google Scholar
  51. 51.
    Petchsomrit A, Sermkaew N, Wiwattanapatapee R (2017) Alginate-based composite sponges as gastroretentive carriers for curcumin-loaded self-microemulsifying drug delivery systems. Sci Pharm 85(1):11CrossRefGoogle Scholar
  52. 52.
    Sarika PR, James NR, Raj DK (2016) Preparation, characterization and biological evaluation of curcumin loaded alginate aldehyde–gelatin nanogels. Mater Sci Eng 68:251–257CrossRefGoogle Scholar
  53. 53.
    Anitha A, Deepagan VG, Rani VD, Menon D, Nair SV, Jayakumar R (2011) Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate–chitosan nanoparticles. Carbohydr Polym 84(3):158–1164CrossRefGoogle Scholar
  54. 54.
    Sarika PR, James NR (2016) Polyelectrolyte complex nanoparticles from cationised gelatin and sodium alginate for curcumin delivery. Carbohydr Polym 148:354–361CrossRefGoogle Scholar
  55. 55.
    Akolade JO, Oloyede HOB, Salawu MO, Amuzat AO, Ganiyu AI, Onyenekwe PC (2018) Influence of formulation parameters on encapsulation and release characteristics of curcumin loaded in chitosan-based drug delivery carriers. J Drug Delivery Sci Technol 45:11–19CrossRefGoogle Scholar
  56. 56.
    Akolade JO, Oloyede HOB, Onyenekwe PC (2017) Encapsulation in chitosan-based polyelectrolyte complexes enhances antidiabetic activity of curcumin. J Funct Foods 35:584–594CrossRefGoogle Scholar
  57. 57.
    Almeida EAMS, Bellettini IC, Garcia FP, Farinácio MT, Nakamura CV, Rubira AF, Martins AF, Muniz EC (2017) Curcumin-loaded dual pH- and thermo-responsive magnetic microcarriers based on pectin maleate for drug delivery. Carbohydr Polym 171:259–266CrossRefGoogle Scholar
  58. 58.
    Zamarioli CM, Martins RM, Carvalho EC, Freitas LAP (2015) Nanoparticles containing curcuminoids (Curcuma longa):development of topical delivery formulation. Rev Bras 25:53–60Google Scholar
  59. 59.
    Ning P, Lü S, Bai X, Wu X, Gao C, Wen N, Liu M (2018) High encapsulation and localized delivery of curcumin from an injectable hydrogel. Mater Sci Eng 83:121–129CrossRefGoogle Scholar
  60. 60.
    Wang H, Gong X, Guo X, Liu C, Fan YY, Zhang J, Li W (2018) Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads. Int J Biol Macromol 121:1118–1125CrossRefGoogle Scholar
  61. 61.
    Upadhyaya L, Singh J, Agarwal V, Pandey AC, Verma SP, Das P, Tewari RP (2014) In situ grafted nanostructured ZnO/carboxymethyl cellulose nanocomposites for efficient delivery of curcumin to cancer. J Polym Res 21:550CrossRefGoogle Scholar
  62. 62.
    Manatunga DC, de Silva RM, de Silva KN, de Silva N, Bhandari S, Yap YK, Costha NP (2017) pH responsive controlled release of anti-cancer hydrophobic drugs from sodium alginate and hydroxyapatite bi-coated iron oxide nanoparticles. Eur J Pharm Biopharm 117:29–38CrossRefGoogle Scholar
  63. 63.
    Zhou M, Hu Q, Wang T, Xue J, Luo Y (2018) Alginate hydrogel beads as a carrier of low-density lipoprotein/pectin nanogels for potential oral delivery applications. Int J Biol Macromol 120:859–864CrossRefGoogle Scholar

Copyright information

© The Polymer Society, Taipei 2018

Authors and Affiliations

  • Ching-Hwa Lee
    • 1
  • Lakshmi P. Nalluri
    • 1
    Email author
  • Srinivasa R. Popuri
    • 2
  1. 1.Department of Environmental EngineeringDa-Yeh UniversityChanghuaRepublic of China
  2. 2.Department of Biological & Chemical SciencesThe University of the West IndiesBridgetownBarbados

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