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Journal of Polymer Research

, 22:184 | Cite as

Effect of dendritic polymers on a simple model biological membrane

  • K. Ciepluch
  • B. Nyström
  • D. Appelhans
  • M. Zablocka
  • M. Bryszewska
  • J. P. Majoral
Original Paper
  • 265 Downloads

Abstract

The higher disruption of lipid bilayer and higher cytotoxicity caused by nanoparticles are often linked to the aggregation process of the particles onto the biological membrane of the cell. To understand how nanoparticles behave when they meet a biological membrane it is important to consider their potential applications in biomedical science. In spite of many biological applications of dendritic scaffolds, a major problem of their cationic exponents is the disruptive biological properties against cell membranes leading to toxicity. Up to now many studies about biological and biochemical effects on membranes have emerged. Therefore, we have investigated the interaction of different kinds of dendritic scaffols on a simple biological membrane model: a small cationic dendrimer with viologen units, hyperbranched poly(ethyleneimine) (PEI), and maltosylated PEI. Our main aim was to check how different types of dendritic scaffolds can affect the model membrane. To probe this effect, we employed dynamic light scattering (DLS), zeta potential, fluorescence, and transmission electron microscopy (TEM) techniques. Results from this work facilitate our understanding of interaction of dendritic nanoparticles with biological membranes. This investigation can help use to explain the toxicological effect of these nanoparticles. As a model of a biological membrane, we use negatively charged liposomes, consisting of a mixture of lipids: 90 % DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) and 10 % DPPG (1,2-Dipalmitoyl-sn-glycero-3-phosphoglycerol).

Keywords

Dendritic polymers Liposomes Dynamic light scattering Membrane model 

Notes

Acknowledgments

The authors thank Antje Hofgaard for his assistance during experiments with Transmision Electron Microscopy. Studies were partially funded by the Ministry of Science and Higher Education in program ,,Mobility plus”.

Conflict of interest

The authors do not have commercial or other associations that might pose a conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • K. Ciepluch
    • 1
    • 2
  • B. Nyström
    • 2
  • D. Appelhans
    • 3
  • M. Zablocka
    • 4
  • M. Bryszewska
    • 1
  • J. P. Majoral
    • 5
  1. 1.Department of General BiophysicsUniversity of LodzLodzPoland
  2. 2.Department of ChemistryUniversity of OsloBlindernNorway
  3. 3.Leibniz Institute of Polymer Research DresdenDresdenGermany
  4. 4.Centre of Molecular and Macromolecular StudiesPolish Academy of ScienceLodzPoland
  5. 5.Laboratorie de Chimie de Coordination, CNRSToulouseFrance

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