Journal of Polymer Research

, 13:323

Properties of a Calcium Phosphate Cement Synergistically Reinforced by Chitosan Fiber and Gelatin

  • Zhao Hui Pan
  • He Ping Cai
  • Ping Ping Jiang
  • Qing Yu Fan
Communication

DOI: 10.1007/s10965-006-9041-2

Cite this article as:
Pan, Z.H., Cai, H.P., Jiang, P.P. et al. J Polym Res (2006) 13: 323. doi:10.1007/s10965-006-9041-2

Abstract

Calcium phosphate cement (CPC) composite by the incorporation of chitosan fiber (CF) and gelatin was investigated for flexural strength, phase composition, microstructure, and in vitro cellular response. The results demonstrated that the incorporation improved the flexural strength significantly (maximum, 12.31 MPa), together with accelerating the conversion of CPC components to hydroxyapatite (HA). Rat bone marrow stromal cells (RBMSCs) spread and grew actively on the composite discs. Short-term minimum essential medium (MEM) extraction tests showed no cell growth inhibition. These results indicated that the new composite not only had a reinforced flexural property, but it was also beneficial for RBMSC growth.

Key words

calcium phosphate cement chitosan cytotoxicity flexural strength gelatin 

Abbreviations

CPC

Calcium phosphate cement

CS

Chitosan

CF

Chitosan fiber

RBMSCs

Rat bone marrow stromal cells

TTCP

Tetracalcium phosphate

DCPA

Dicalcium phosphate anhydrous

XRD

X-ray diffraction

SEM

Scanning electron microscopy

DMEM

Dulbecco's modified Eagle medium

FBS

Fetal bovine serum

PBS

Phosphate-buffered saline

GFP

Green fluorescence protein

TCPS

Tissue culture polystyrene

MEM

Minimum essential medium

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

DMSO

Dimethyl sulfoxide

OD

Optical density

ANOVA

One-way analysis of variance

HA

Hydroxyapatite

JCPDS

Joint committee on powder diffraction standards

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Zhao Hui Pan
    • 1
  • He Ping Cai
    • 2
  • Ping Ping Jiang
    • 3
  • Qing Yu Fan
    • 1
  1. 1.Orthopedics Oncology Institute of Chinese PLA, Tangdu HospitalFourth Military Medical UniversityXi'anPeople's Republic of China
  2. 2.State Key Laboratory for Mechanical Behavior of MaterialsXi'an Jiaotong UniversityXi'anPeople's Republic of China
  3. 3.Orthopedics Institute of Chinese PLA89th HospitalWeifangPeople's Republic of China

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