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Journal of Solution Chemistry

, Volume 38, Issue 4, pp 449–458 | Cite as

Buffer Standards for pH Measurement of N-(2-Hydroxyethyl)piperazine-N′-2-ethanesulfonic Acid (HEPES) for I=0.16 mol⋅kg−1 from 5 to 55 °C

  • Rabindra N. Roy
  • Lakshmi N. Roy
  • Shahaf Ashkenazi
  • Joshua T. Wollen
  • Craig D. Dunseth
  • Michael S. Fuge
  • Jared L. Durden
  • Chandra N. Roy
  • Hannah M. Hughes
  • Brett T. Morris
  • Kevin L. Cline
Article

Abstract

The values of the second dissociation constant, pK 2, of N-(2-hydroxyethyl) piperazine-N′-2-ethanesulfonic acid (HEPES) have been reported at twelve temperatures over the temperature range 5 to 55 °C, including 37 °C. This paper reports the results for the pa H of eight isotonic saline buffer solutions with an I=0.16 mol⋅kg−1 including compositions: (a) HEPES (0.01 mol⋅kg−1) + NaHEPES (0.01 mol⋅kg−1) + NaCl (0.15 mol⋅kg−1); (b) HEPES (0.02 mol⋅kg−1) + NaHEPES (0.02 mol⋅kg−1) + NaCl (0.14 mol⋅kg−1); (c) HEPES (0.03 mol⋅kg−1) + NaHEPES (0.03 mol⋅kg−1) + NaCl (0.13 mol⋅kg−1); (d) HEPES (0.04 mol⋅kg−1) + NaHEPES (0.04 mol⋅kg−1) + NaCl (0.12 mol⋅kg−1); (e) HEPES (0.05 mol⋅kg−1) + NaHEPES (0.05 mol⋅kg−1) + NaCl (0.11 mol⋅kg−1); (f) HEPES (0.06 mol⋅kg−1) + NaHEPES (0.06 mol⋅kg−1) + NaCl (0.10 mol⋅kg−1); (g) HEPES (0.07 mol⋅kg−1) + NaHEPES (0.07 mol⋅kg−1) + NaCl (0.09 mol⋅kg−1); and (h) HEPES (0.08 mol⋅kg−1) + NaHEPES (0.08 mol⋅kg−1) + NaCl (0.08 mol⋅kg−1). Conventional pa H values, for all eight buffer solutions from 5 to 55 °C, have been calculated. The operational pH values with liquid junction corrections, at 25 and 37 °C have been determined based on the NBS/NIST standard between the physiological phosphate standard and four buffer solutions. These are recommended as pH standards for physiological fluids in the range of pH = 7.3 to 7.5 at I=0.16 mol⋅kg−1.

Keywords

Buffers HEPES Ionic strength Emf Zwitterions pH Acidity function 

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Rabindra N. Roy
    • 1
  • Lakshmi N. Roy
    • 1
  • Shahaf Ashkenazi
    • 1
  • Joshua T. Wollen
    • 1
  • Craig D. Dunseth
    • 1
  • Michael S. Fuge
    • 1
  • Jared L. Durden
    • 1
  • Chandra N. Roy
    • 1
  • Hannah M. Hughes
    • 1
  • Brett T. Morris
    • 1
  • Kevin L. Cline
    • 1
  1. 1.Hoffman Department of ChemistryDrury UniversitySpringfieldUSA

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