Journal of Solution Chemistry

, 37:1593 | Cite as

An Alternate Solution of Fluorescence Recovery Kinetics after Spot-Bleaching for Measuring Diffusion Coefficients. 2. Diffusion of Fluorescein in Aqueous Sucrose Solutions

  • H. R. CortiEmail author
  • G. A. Frank
  • M. C. Marconi


The traditional analysis of the fluorescence recovery kinetics after spot bleaching yields expressions for the diffusion coefficient of the probe that are not suitable for linear fittings. In a previous work we developed an improved recovery function that is a better alternative for data analysis. To illustrate its application to real cases and compare it with the previous data treatment, we measured the time response of fluorescein in aqueous sucrose solutions, covering the unsaturated and the supercooled region, where decoupling between diffusion and viscosity is observed. The results are compared with the mobility of different types of solutes in aqueous sucrose solutions and are discussed in terms of the classical hydrodynamic model.


Photobleaching Fluorescence recovery Diffusion Fluorescein Supercooled Sucrose solutions Stokes-Einstein equation 


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© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Departamento de Fisica de Materia Condensada, Centro Atómico ConstituyentesComisión Nacional de Energía AtómicaBuenos AiresArgentina
  2. 2.Instituto de Química Física de los Materiales, Ambiente y Energía (INQUIMAE), Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresBuenos AiresArgentina
  3. 3.Laboratorio de Electrónica Cuántica, Departamento de Física, Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresBuenos AiresArgentina

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