Journal of Porous Materials

, Volume 22, Issue 5, pp 1215–1226 | Cite as

Bi-layered porous constructs of PCL-coated 45S5 bioactive glass and electrospun collagen-PCL fibers

  • Preethi Balasubramanian
  • Judith A. Roether
  • Dirk W. Schubert
  • Justus P. Beier
  • Aldo R. Boccaccini
Article

Abstract

A simple yet promising approach to construct bi-layered scaffolds using bioactive ceramics and biodegradable polymers is presented. This method involves two versatile fabrication techniques used in the field of TE: foam replication process and electrospinning. By the foam replication method, three-dimensional 45S5 bioactive glass (BG)-based scaffolds with high porosity, in the range of 95.8 ± 0.9 %, were produced. To improve the mechanical properties of the BG scaffolds, dip-coating using polycaprolactone (PCL) was performed, which led to a significant increase in the compressive strength of the scaffolds. In order to develop a bi-layered construct, bead-less submicrometric fibers of collagen-PCL were electrospun over the PCL-coated BG scaffolds. Surface morphology, surface properties and mechanical strength of the bi-layered construct were evaluated using scanning electron microscopy analysis, contact angle measurements and compressive strength testing, respectively. In vitro degradation of the collagen-PCL fibers in phosphate buffered saline and in vitro bioactivity of the bi-layered constructs in simulated body fluid were investigated. Formation of hydroxyapatite on the PCL-coated BG and along the morphology of the collagen-PCL fibers was ascertained using different characterization techniques. The bi-layered construct is intended for interface tissue engineering applications where the PCL-coated BG scaffold, which is highly bioactive, can serve as a support for the bone side and the composite collagen-PCL submicrometric fibers are intended for the cartilage side.

Keywords

Osteochondral region Electrospinning In vitro degradation Bioactivity Scaffold 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Preethi Balasubramanian
    • 1
  • Judith A. Roether
    • 2
  • Dirk W. Schubert
    • 2
  • Justus P. Beier
    • 3
  • Aldo R. Boccaccini
    • 1
  1. 1.Department of Materials Science and Engineering, Institute of BiomaterialsUniversity of Erlangen-NurembergErlangenGermany
  2. 2.Department of Materials Science and Engineering, Institute of Polymer MaterialsUniversity of Erlangen-NurembergErlangenGermany
  3. 3.Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital ErlangenUniversity of Erlangen-NurembergErlangenGermany

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