Bi-layered porous constructs of PCL-coated 45S5 bioactive glass and electrospun collagen-PCL fibers
A simple yet promising approach to construct bi-layered scaffolds using bioactive ceramics and biodegradable polymers is presented. This method involves two versatile fabrication techniques used in the field of TE: foam replication process and electrospinning. By the foam replication method, three-dimensional 45S5 bioactive glass (BG)-based scaffolds with high porosity, in the range of 95.8 ± 0.9 %, were produced. To improve the mechanical properties of the BG scaffolds, dip-coating using polycaprolactone (PCL) was performed, which led to a significant increase in the compressive strength of the scaffolds. In order to develop a bi-layered construct, bead-less submicrometric fibers of collagen-PCL were electrospun over the PCL-coated BG scaffolds. Surface morphology, surface properties and mechanical strength of the bi-layered construct were evaluated using scanning electron microscopy analysis, contact angle measurements and compressive strength testing, respectively. In vitro degradation of the collagen-PCL fibers in phosphate buffered saline and in vitro bioactivity of the bi-layered constructs in simulated body fluid were investigated. Formation of hydroxyapatite on the PCL-coated BG and along the morphology of the collagen-PCL fibers was ascertained using different characterization techniques. The bi-layered construct is intended for interface tissue engineering applications where the PCL-coated BG scaffold, which is highly bioactive, can serve as a support for the bone side and the composite collagen-PCL submicrometric fibers are intended for the cartilage side.
KeywordsOsteochondral region Electrospinning In vitro degradation Bioactivity Scaffold
The authors would like to acknowledge the European Commission funding under the 7th Framework Programme (Marie Curie Initial Training Networks; Grant Number: 289958, Bioceramics for bone repair). The authors thank Dr. Patcharakamon Nooeaid, Ms Yaping Ding, Mr Wei Li and Dr Menti Goudouri (Institute of Biomaterials, University of Erlangen-Nuremberg) for experimental support.
Conflict of interest
The authors declare that they have no conflict of interest.
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