Journal of Porous Materials

, Volume 17, Issue 5, pp 605–613

Degradable and bioactive scaffold of calcium phosphate and calcium sulphate from self-setting cement for bone regeneration

  • Gangfeng Hu
  • Luwei Xiao
  • Hong Fu
  • Dawei Bi
  • Haitao Ma
  • Peijian Tong


Calcium sulphate/phosphate cement (CSPC) porous scaffolds were fabricated by introduction of calcium sulphate (CS) into calcium phosphate cement utilizing particle-leaching method. The morphology, porosity and mechanical strength as well as degradation of the CSPC scaffolds were characterized. The results reveal that the CSPC with 40 wt% CS content (40 CSPC) scaffolds with a porosity of 81% showed open macropores with the pore size of 200–500 μm. In addition, the 40 CSPC scaffolds with good degree of interconnected macropores degraded 60 wt% in Tris–HCl solution after 12 weeks. The proliferation, differentiation and morphology of MG63 cells on the 40 CSPC scaffolds were determined using MTT assay, ALP activity and SEM. The results suggest that the CSPC scaffolds could stimulate cell proliferation and differentiation, indicating that CSPC scaffolds were biocompatible and had no negative effects on the cells in vitro. The CSPC scaffolds were implanted in femur bone defect of rabbits, and the in vivo biocompatibility and osteogenicity of the scaffolds were investigated. The results indicate that CSPC scaffolds exhibited good biocompatibility, degradability and osteogenesis in vivo.


Calcium phosphate Calcium sulphate Porous scaffolds Degradability Osteogenesis 


  1. 1.
    H.H.K. Xu, S. Takagi, J.B. Quinn, J. Biomed. Mater. Res. A 68, 725 (2004)CrossRefGoogle Scholar
  2. 2.
    J.E. Barralet, L. Grover, T. Gaunt, A.J. Wright, I.R. Gibson, Biomaterials 23, 3063 (2002)CrossRefGoogle Scholar
  3. 3.
    Z.D. Xia, L.M. Grover, Y.Z. Huang, L.E. Adamopoulos, U. Gbureck, J.T. Triffitt et al., Biomaterials 27, 4557 (2006)CrossRefGoogle Scholar
  4. 4.
    C.S. Liu, H.F. Shao, F.Y. Chen, H.Y. Zheng, Biomaterials 24, 4103 (2003)CrossRefGoogle Scholar
  5. 5.
    S. del Valle, N. Mino, F. Munoz, A. Gonzalez, J.A. Planell, M.P. Ginebra, J. Mater. Sci: Mater. Med. 18, 353 (2007)CrossRefGoogle Scholar
  6. 6.
    S. Takagi, L.C. Chow, J. Mater. Sci: Mater. Med. 12, 135 (2001)CrossRefGoogle Scholar
  7. 7.
    A.R. Michael, A.W. Thomas, S. Volker, D. Elvira, Z. Ludwig, R. Schnettler et al., Biomaterials 26, 2677 (2005)CrossRefGoogle Scholar
  8. 8.
    A.S. Coetzee, Arch Otolaryngol. 106, 405 (1980)Google Scholar
  9. 9.
    S. Gitelis, P. Piasecki, T. Turner, W. Haggard, J. Charters, R. Urban, Orthopedics 24, 162 (2001)Google Scholar
  10. 10.
    D.I. Alexander, N.A. Manson, M.J. Mitchell, J. Surg. 44, 262 (2001)Google Scholar
  11. 11.
    M. Bohner, Biomaterials 25, 741 (2004)CrossRefGoogle Scholar
  12. 12.
    H. Guo, J. Wei, C.S. Liu, Biomed. Mater. 1, 193 (2006)CrossRefGoogle Scholar
  13. 13.
    M. Nilsson, E. Fernandez, S. Sarda, L. Lidgren, J.A. Planell, J. Biomed. Mater. Res. 61, 600 (2002)CrossRefGoogle Scholar
  14. 14.
    E. Fernandez, M.D. Vlad, M.M. Gel, J. Lopez, R. Torres, J.V. Cauich, M. Bohner, Biomaterials 26, 3395 (2005)CrossRefGoogle Scholar
  15. 15.
    K. Rezwan, Q.Z. Chen, J.J. Blaker, Biomaterials 27, 3413 (2006)CrossRefGoogle Scholar
  16. 16.
    C.S. Liu, W. Gai, S.H. Pan, Z.S. Liu, Biomaterials 24, 2995 (2003)CrossRefGoogle Scholar
  17. 17.
    E.W. Bodde, C.T. Cammaert, J.G. Wolke, P.H. Spauwen, J.A. Jansen, J. Biomed. Mater. Res. 83, 161 (2007)CrossRefGoogle Scholar
  18. 18.
    W. Huanan, L. Yubao, Z. Yi, L. Jihua, M. Sansi, C. Lin, Biomaterials 28, 3338 (2007)CrossRefGoogle Scholar
  19. 19.
    H. Yuan, K. Kurashina, K. de Groot, X. Zhang, Biomaterials 20, 1799 (1999)CrossRefGoogle Scholar
  20. 20.
    P. Link Dennis, J. Van Den Dolder, J.J.J.P. Van Den Beucken, V.M. Cuijpers, J.A. Jansen et al., J. Biomed. Mater. Res. A. 87, 760 (2008)Google Scholar
  21. 21.
    H. Sun, C. Wu, K. Dai, J. Chang, T. Tang, Biomaterials 27, 5651 (2006)CrossRefGoogle Scholar
  22. 22.
    I.D. Xynos, A.J. Edgar, L.D.K. Buttery, L.L. Hench, J.M. Polak, J. Biomed. Mater. Res. A 55, 151 (2001)CrossRefGoogle Scholar
  23. 23.
    Y.F. Chou, W.B. Huang, J.C.Y. Dunn, T.A. Miller, B.M. Wu, Biomaterials 26, 285 (2005)CrossRefGoogle Scholar
  24. 24.
    M. Khadra, S.P. Lyngstadaas, H.R. Haanase, K. Mustafa, Biomaterials 26, 3503 (2005)CrossRefGoogle Scholar
  25. 25.
    R.P. del Real, E. Ooms, J.G. Wolke, M. Vallet-Regi, J.A. Jansen, J. Biomed. Mater. Res. 65, 30 (2003)CrossRefGoogle Scholar
  26. 26.
    M. Julien, I. Khairoun, R.Z. LeGeros, S. Delplace, P. Pilet, P. Weiss, Biomaterials 28, 956 (2007)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Gangfeng Hu
    • 1
    • 2
  • Luwei Xiao
    • 2
  • Hong Fu
    • 1
  • Dawei Bi
    • 1
  • Haitao Ma
    • 1
  • Peijian Tong
    • 2
  1. 1.The First People’s Hospital of XiaoshangHangzhouChina
  2. 2.Zhejiang Traditional Chinese Medical UniversityHangzhouChina

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