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Journal of Porous Materials

, Volume 17, Issue 3, pp 313–320 | Cite as

Preparation, characterization and mechanical properties of controlled porous gelatin/hydroxyapatite nanocomposite through layer solvent casting combined with freeze-drying and lamination techniques

  • Mahmoud AzamiEmail author
  • Fathollah Moztarzadeh
  • Mohammadreza Tahriri
Article

Abstract

In this present study, to mimic the mineral and organic component of natural bone, hydroxyapatite (HA) and gelatin (GEL) nanocomposite was prepared via layer solvent casting combined with freeze-drying and lamination techniques. Glutaraldehyde (GA) was used as cross-linking agent. The synthesized nanocrystalline hydroxyapatite and nanocomposite samples were characterized by the commonly used bulk techniques. The results showed that GEL/HA nanocomposite were porous with 3-dimension interconnected microstructure, pore sizes were 100 μm to 1 mm, porosity were 75% to 93% and HA particles are dispersed evenly among gelatin fibers. It was also found that increasing initial GEL concentration and HA content enhance the elastic modulus (E) and reduce toughness and affect pore size and morphology. Finally, the stress–strain behavior in compression was very similar to natural spongy bone where the compressive modulus obtained was about 180 MPa.

Keywords

Nanocomposite Gelatin Hydroxyapatite Freeze-drying Lamination technique 

References

  1. 1.
    R. Langer, J.P. Vacanti, Science 260, 920 (1993). doi: 10.1126/science.8493529 CrossRefGoogle Scholar
  2. 2.
    F.G. Heineken, R. Skalak, J. Biomech. Eng. 113, 111 (1991). doi: 10.1115/1.2891223 CrossRefGoogle Scholar
  3. 3.
    R.M. Nerem, Ann. Biomed. Eng. 19, 529 (1991). doi: 10.1007/BF02367396 CrossRefGoogle Scholar
  4. 4.
    K.T. Paige, C.A. Vacanti, Tissue Eng. 1, 97 (1995). doi: 10.1089/ten.1995.1.97 CrossRefGoogle Scholar
  5. 5.
    E.B. Denkbas, M. Seyyal, E. Piskin, J. Membr. Sci. 172, 33 (2000). doi: 10.1016/S0376-7388(00)00314-8 CrossRefGoogle Scholar
  6. 6.
    C.L. Tseng, S.Y. Wu, W.H. Wang, C.L. Peng, F.H. Lin, C.C. Lin, T.H. Young, M.J. Shieh, Biomaterials 29, 3014 (2008). doi: 10.1016/j.biomaterials.2008.03.033 CrossRefGoogle Scholar
  7. 7.
    G.E. Means, R.E. Feeney, J. Food Chem. 22, 399 (1998)Google Scholar
  8. 8.
    F.H. Jones, Surf. Sci. Rep. 42, 75 (2001). doi: 10.1016/S0167-5729(00)00011-X CrossRefGoogle Scholar
  9. 9.
    M. Kikuchi, S. Itoh, S. Ichinose, K. Shinomiya, J. Tanaka, Biomaterials 22, 1705 (2001). doi: 10.1016/S0142-9612(00)00305-7 CrossRefGoogle Scholar
  10. 10.
    R. Schnettler, V. Alt, E. Dingeldein, H.J. Pfefferle, O. Kilian, C. Meyer, C. Heiss, S. Wenisch, Biomaterials 24, 4603 (2003). doi: 10.1016/S0142-9612(03)00354-5 CrossRefGoogle Scholar
  11. 11.
    S.C. Liou, S.Y. Chen, D.M. Liu, Biomaterials 24, 3981 (2003). doi: 10.1016/S0142-9612(03)00303-X CrossRefGoogle Scholar
  12. 12.
    E. Mavropoulos, A.M. Rossi, N.C.C. Rocha, G.A. Soares, J.C. Moreira, G.T. Moure, Mater. Charact. 50, 203 (2003). doi: 10.1016/S1044-5803(03)00093-7 CrossRefGoogle Scholar
  13. 13.
    Y. Li, C.P.A.T. Klein, J. Mater. Sci. Mater. Med. 5, 263 (1994). doi: 10.1007/BF00120372 CrossRefGoogle Scholar
  14. 14.
    J. Torrent-Burgues, J. Gomez-Morales, A. Lopez-Macipe, R. Rodriguez-Clemente, Cryst. Res. Technol. 34, 757 (1999). doi: 10.1002/(SICI)1521-4079(199906)34:5/6<757::AID-CRAT757>3.0.CO;2-L CrossRefGoogle Scholar
  15. 15.
    R. Murugan, S. Ramakrishna, Compos. Sci. Technol. 65, 2385 (2005). doi: 10.1016/j.compscitech.2005.07.022 CrossRefGoogle Scholar
  16. 16.
    S. Itoh, M. Kikuchi, Y. Koyama, H.N. Matumoto, K. Takakuda, K. Shinomiya, J. Biomed. Mater. Eng. 15, 29 (2005)Google Scholar
  17. 17.
    K.B. Masanori, N. Hiroko, C. Matsumotob, Y. Takeki, K. Yoshihisa, T. Kazuo, T. Junzo, Biomaterials 25, 63 (2004). doi: 10.1016/S0142-9612(03)00472-1 CrossRefGoogle Scholar
  18. 18.
    I. Yamaguchi, K. Tokuchi, H. Fukuzaki, Y. Koyama, K. Takakada, H. Monma, J. Biomed. Mater. Res. 55, 20 (2001). doi: 10.1002/1097-4636(200104)55:1<20::AID-JBM30>3.0.CO;2-F CrossRefGoogle Scholar
  19. 19.
    S.S. Liao, F.Z. Cui, X.D. Zhu, J. Bioact. Compat. Polym. 19, 117 (2004). doi: 10.1177/0883911504042643 CrossRefGoogle Scholar
  20. 20.
    S.M. Zhang, F.Z. Cui, S.S. Liao, Y. Zhu, L. Han, J. Mater. Sci.: Mater. Med. 14, 641 (2003). doi: 10.1023/A:1024083309982 CrossRefGoogle Scholar
  21. 21.
    K. Hae-Won, C.K. Jonathan, K. Hyoun-Ee, J. Biomed. Mater. Res. 72, 136 (2005)Google Scholar
  22. 22.
    A. Bigi, E. Boanini, S. Panzavolta, N. Roveri, K. Rubini, J. Biomed. Mater. Res. 59, 709 (2002). doi: 10.1002/jbm.10045 CrossRefGoogle Scholar
  23. 23.
    C.C. Myung, K. Ching-Chang, H.D. William, Biomaterials 24, 2853 (2003). doi: 10.1016/S0142-9612(03)00115-7 CrossRefGoogle Scholar
  24. 24.
    K. Hae-Won, K. Hyoun-Ee, S. Vehid, Biomaterials 26, 5221 (2005). doi: 10.1016/j.biomaterials.2005.01.047 CrossRefGoogle Scholar
  25. 25.
    C.C. Chang, J. Tanaka, Biomaterials 23, 4811 (2002). doi: 10.1016/S0142-9612(02)00232-6 CrossRefGoogle Scholar
  26. 26.
    M.C. Chang, J. Tanaka, Biomaterials 23, 3879 (2002). doi: 10.1016/S0142-9612(02)00133-3 CrossRefGoogle Scholar
  27. 27.
    J.G. Morales, J.T. Burgues, T. Boix, J. Fraile, R.R. Clemente, Cryst. Res. Technol. 36, 15 (2001). doi: 10.1002/1521-4079(200101)36:1<15::AID-CRAT15>3.0.CO;2-E CrossRefGoogle Scholar
  28. 28.
    N. Rameshbabu, T.S.S. Kumar, K. Prasad Rao, Bull. Mater. Sci. 29, 611 (2006). doi: 10.1007/s12034-006-0012-3 CrossRefGoogle Scholar
  29. 29.
    M. Komath, H.K. Varma, Bull. Mater. Sci. 4, 415 (2003). doi: 10.1007/BF02711186 CrossRefGoogle Scholar
  30. 30.
    M. Wei, J.H. Evans, T. Bostrom, L. Grondahl, J. Mater. Sci.: Mater. Med. 14, 311 (2003). doi: 10.1023/A:1022975730730 CrossRefGoogle Scholar
  31. 31.
    A. Afshar, M. Ghorbani, N. Ehsani, M.R. Saeri, C.C. Sorrell, Mater. Des. 24, 197 (2003)Google Scholar
  32. 32.
    M.G.S. Murray, J. Wang, C.B. Pontoon, P.M. Marquis, J. Mater. Sci. 30, 3061 (1995). doi: 10.1007/BF01209218 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Mahmoud Azami
    • 1
    Email author
  • Fathollah Moztarzadeh
    • 1
  • Mohammadreza Tahriri
    • 1
  1. 1.Faculty of Biomedical Engineering, Biomaterial GroupAmirkabir University of TechnologyTehranIran

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